DACT1 (NM_001079520) Human Untagged Clone

CAT#: SC315620

DACT1 (untagged)-Human dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis) (DACT1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DACT1
• Synonyms: DAPPER; DAPPER1; DPR1; FRODO; HDPR1; TBS2; THYEX3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001079520, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGCCGAGTCCGGCCGGGACGGCGAAGGAGCTGGAGCCTCCGGCGCCGGCCCGAGGCGAGCAGCGCA
  CGGCGGAGCCCGAGGGGCGCTGGCGGGAGAAGGGCGAGGCAGACACCGAGCGGCAGCGCACCCGGGAGCG
  GCAGGAGGCCACGCTGGCCGGGCTGGCGGAGCTGGAGTACCTGCGCCAGCGCCAAGAGCTGCTGGTCAGG
  GGCGCCCTGCGCGGCGCCGGGGGTGCGGGAGCCGCTGCGCCCCGCGCTGGGGAGCTACTGGGGGAGGCGG
  CGCAGCGCAGTCGCCTGGAGGAGAAGTTCTTGGAGGAGAACATCTTGCTGCTAAGAAAGCAATTGAACTG
  TTTGAGGCGAAGAGATGCTGGTTTGTTGAATCAGTTGCAAGAGCTTGACAAGCAGATAAGTGACCTGAGA
  CTGGATGTAGAAAAGACATCTGAAGAGCACCTGGAGACAGACAGTCGGCCTAGCTCAGGGTTTTATGAGC
  TGAGTGATGGGGCTTCAGGATCCCTTTCCAATTCCTCTAACTCGGTGTTCAGTGAGTGTTTATCCAGTTG
  TCATTCCAGCACCTGCTTTTGCAGCCCCTTGGAGGCGACCTTGAGTCTCTCAGATGGTTGCCCCAAATCT
  GCAGATGTGAATCCCAAGTACCAGTGTGATCTGGTGTCTAAAAACGGGAATGATGTATATCGCTATCCCA
  GTCCACTTCATGCTGTGGCTGTGCAGAGCCCAATGTTTCTCCTTTGTCTGACGGGCAACCCTCTGAGGGA
  AGAGGACAGGCTTGGAAACCATGCCAGTGACATTTGCGGTGGATCTGAGCTAGATGCCGTCAAAACAGAC
  AGTTCCTTACCGTCCCCAAGCAGTCTGTGGTCTGCTTCCCATCCTTCATCCAGCAAGAAAATGGATGGCT
  ACATTCTGAGCCTGGTCCAGAAAAAAACACACCCTGTAAGGACCAACAAACCAAGAACCAGCGTGAACGC
  TGACCCCACGAAAGGGCTTCTGAGGAACGGGAGCGTTTGTGTCAGAGCCCCGGGCGGTGTCTCACAGGGC
  AACAGTGTGAACCTTAAGAATTCGAAACAGGCGTGTCTGCCCTCTGGCGGGATACCTTCTCTGAACAATG
  GGACATTCTCCCCACCGAAGCAGTGGTCGAAAGAATCAAAGGCCGAACAAGCCGAAAGCAAGAGGGTGCC
  CCTGCCAGAGGGCTGCCCCTCAGGCGCTGCCTCCGACCTTCAGAGTAAGCACCTGCCAAAAACGGCCAAG
  CCAGCCTCGCAAGAACATGCTCGGTGTTCCGCCATTGGGACAGGGGAGTCCCCTAAGGAAAGCGCTCAGC
  TCTCAGGGGCCTCTCCAAAAGAGAGTCCTAGCAGAGGCCCTGCCCCGCCGCAGGAGAACAAAGTTGTACA
  GCCCCTGAAAAAGATGTCACAGAAAAACAGCCTGCAGGGCGTCCCCCCGGCCACTCCTCCCCTGCTGTCT
  ACAGCTTTCCCCGTGGAAGAGAGGCCTGCCTTGGATTTCAAGAGCGAGGGCTCTTCCCAAAGCCTGGAGG
  AAGCGCACCTGGTCAAGGCCCAGTTTATCCCGGGGCAGCAGCCCAGTGTCAGGCTCCACCGGGGCCACAG
  GAACATGGGCGTCGTGAAGAACTCCAGCCTGAAGCACCGCGGCCCAGCCCTCCAGGGGCTGGAGAACGGC
  TTGCCCACCGTCAGGGAGAAAACGCGGGCCGGGAGCAAGAAGTGTCGCTTCCCAGATGACTTGGATACAA
  ATAAGAAACTCAAGAAAGCCTCCTCCAAGGGGAGGAAGAGTGGGGGCGGGCCCGAGGCTGGTGTTCCCGG
  CAGGCCCGCGGGCGGGGGCCACAGGGCGGGGAGCAGGGCGCATGGCCACGGACGGGAGGCGGTGGTGGCC
  AAACCTAAGCACAAGCGAACTGACTACCGGCGGTGGAAGTCCTCGGCCGAGATTTCCTACGAAGAGGCCC
  TGAGGAGGGCCCGGCGCGGTCGCCGGGAGAATGTGGGGCTGTACCCCGCGCCTGTGCCTCTGCCCTACGC
  CAGCCCCTACGCCTACGTGGCTAGCGACTCCGAGTACTCGGCCGAGTGCGAGTCCCTGTTCCACTCCACC
  GTGGTGGACACCAGTGAGGACGAGCAGAGCAATTACACCACCAACTGCTTCGGGGACAGCGAGTCGAGTG
  TGAGCGAGGGCGAGTTCGTGGGGGAGAGCACAACCACCAGCGACTCTGAAGAAAGCGGGGGCTTAATTTG
  GTCCCAGTTTGTCCAGACTCTGCCCATTCAAACGGTAACGGCCCCAGACCTTCACAACCACCCCGCAAAA
  ACCTTTGTCAAAATTAAGGCCTCACATAACCTCAAGAAGAAGATCCTCCGCTTTCGGTCTGGCTCTTTGA
  AACTGATGACGACGGTTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001079520
• ORF Size: 2400 bp
• Insert Size: 0
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001079520.1, NP_001072988.1
• RefSeq Size: 3766
• RefSeq ORF: 2400
• Locus ID: 51339
• Gene Summary: The protein encoded by this gene belongs to the dapper family, characterized by the presence of PDZ-binding motif at the C-terminus. It interacts with, and positively regulates dishevelled-mediated signaling pathways during development. Depletion of this mRNA from xenopus embryos resulted in loss of notochord and head structures, and mice lacking this gene died shortly after birth from severe posterior malformations. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at the 3' terminal exon compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment compared to isoform 1.