SEC31A (NM_001077208) Human Untagged Clone

CAT#: SC315648

SEC31A (untagged)-Human SEC31 homolog A (S. cerevisiae) (SEC31A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEC31A
• Synonyms: ABP125; ABP130; HSPC275; HSPC334; SEC31L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001077208, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGTTAAAGGAAGTAGATCGTACAGCCATGCAGGCATGGAGCCCTGCCCAGAATCACCCCATTTACC
  TAGCAACAGGAACATCTGCTCAGCAATTGGATGCAACATTTAGTACGAATGCTTCCCTTGAGATATTTGA
  ATTAGACCTCTCTGATCCATCCTTGGATATGAAATCTTGTGCCACATTCTCCTCTTCTCACAGGTACCAC
  AAGTTGATTTGGGGGCCTTATAAAATGGATTCCAAAGGAGATGTCTCTGGAGTTCTGATTGCAGGTGGTG
  AAAATGGAAATATTATTCTCTATGATCCTTCTAAAATTATAGCTGGAGACAAGGAAGTTGTGATTGCCCA
  GAATGACAAGCATACTGGCCCAGTGAGAGCCTTGGATGTGAACATTTTCCAGACTAATCTGGTAGCTTCT
  GGTGCTAATGAATCTGAAATCTACATATGGGATCTAAATAATTTTGCAACCCCAATGACACCAGGAGCCA
  AAACACAGCCGCCAGAAGATATCAGCTGCATTGCATGGAACAGACAAGTTCAGCATATTTTAGCATCAGC
  CAGTCCCAGTGGCCGGGCCACTGTATGGGATCTTAGAAAAAATGAGCCAATCATCAAAGTCAGTGACCAT
  AGTAACAGAATGCATTGTTCTGGGTTGGCATGGCATCCTGATGTTGCTACTCAGATGGTCCTTGCCTCCG
  AGGATGACCGGTTACCAGTGATCCAGATGTGGGATCTTCGATTTGCTTCCTCTCCACTTCGTGTCCTGGA
  AAACCATGCCAGGGGGATTTTGGCAATTGCTTGGAGCATGGCAGATCCTGAATTGTTACTGAGCTGTGGA
  AAAGATGCTAAGATTCTCTGCTCCAATCCAAACACAGGAGAGGTGTTATATGAACTTCCCACCAACACAC
  AGTGGTGCTTCGATATTCAGTGGTGTCCCCGAAATCCTGCTGTCTTATCAGCTGCTTCGTTTGATGGGCG
  TATCAGTGTTTATTCTATCATGGGAGGAAGCACAGATGGTTTAAGACAGAAACAAGTTGACAAGCTTTCA
  TCATCTTTTGGGAATCTTGATCCCTTTGGCACAGGACAGCCCCTTCCTCCGTTACAAATTCCACAGCAGA
  CTGCTCAGCATAGTATAGTGCTGCCTCTGAAGAAGCCGCCCAAGTGGATTCGAAGGCCTGTTGGTGCTTC
  TTTTTCATTTGGAGGCAAACTGGTTACGTTTGAGAATGTCAGAATGCCTTCTCATCAGGGAGCTGAGCAG
  CAGCAGCAGCAGCACCATGTGTTCATTAGTCAGGTTGTAACAGAAAAGGAGTTCCTCAGCCGATCAGACC
  AACTTCAGCAGGCTGTGCAGTCACAAGGATTTATCAATTATTGCCAAAAAAAAATTGATGCTTCTCAGAC
  TGAATTTGAGAAAAATGTGTGGTCCTTTTTGAAGGTAAACTTTGAGGATGATTCTCGTGGAAAATACCTT
  GAACTTCTAGGATACAGAAAAGAAGATCTAGGAAAGAAGATTGCTTTGGCCTTGAACAAAGTGGATGGAG
  CCAATGTGGCTCTTAAAGACTCTGACCAAGTAGCACAGAGTGATGGGGAGGAGAGCCCTGCTGCTGAAGA
  GCAGCTCTTGGGAGAGCACATTAAAGAGGAAAAAGAAGAATCTGAATTTCTACCCTCATCTGGAGGAACA
  TTTAATATCTCTGTCAGTGGGGACATTGATGGTTTAATTACTCAGGCTTTGCTGACGGGCAATTTTGAGA
  GTGCTGTTGACCTTTGTTTACATGATAACCGCATGGCCGATGCCATTATATTGGCCATAGCAGGTGGACA
  AGAACTCTTGGCTCGAACCCAGAAAAAATACTTCGCAAAATCCCAAAGCAAAATTACCAGGCTCATCACT
  GCAGTGGTGATGAAGAACTGGAAAGAGATTGTTGAGTCTTGTGATCTTAAAAATTGGAGAGAGGCTTTAG
  CTGCAGTATTGACTTATGCAAAGCCGGATGAATTTTCAGCCCTTTGTGATCTTTTGGGAACCAGGCTTGA
  AAATGAAGGAGATAGCCTCCTGCAGACTCAAGCATGTCTCTGCTATATTTGTGCAGGGAATGTAGAGAAA
  TTAGTTGCATGTTGGACTAAAGCTCAAGATGGAAGCCACCCTTTGTCACTTCAGGATCTGATTGAGAAAG
  TTGTCATCCTGCGAAAAGCTGTGCAACTCACTCAAGCCATGGACACTAGTACTGTAGGAGTTCTCTTGGC
  TGCGAAGATGAGTCAGTATGCCAATTTGTTGGCAGCTCAGGGCAGTATTGCTGCAGCCTTGGCTTTTCTT
  CCTGACAACACCAACCAGCCAAATATCATGCAGCTTCGTGACAGACTTTGTAGAGCACAAGGAGAGCCTG
  TAGCAGGACATGAATCACCTAAAATTCCGTACGAGAAACAGCAGCTCCCCAAGGGCAGGCCTGGACCAGT
  TGCTGGCCACCACCAGATGCCAAGAGTTCAAACTCAACAATATTATCCCCATGGAGAAAATCCTCCACCT
  CCGGGTTTCATAATGCATGGAAATGTTAATCCAAATGCTGCTGGTCAGCTTCCCACATCTCCAGGTCATA
  TGCACACCCAGGTACCACCTTATCCACAGCCACAGCCTTATCAACCAGCCCAGCCGTATCCCTTCGGAAC
  AGGGGGGTCAGCAATGTATCGACCTCAGCAGCCTGTTGCTCCTCCTACTTCAAACGCTTACCCTAACACC
  CCTTACATATCTTCTGCTTCTTCCTATACTGGGCAGTCTCAGCTGTACGCAGCACAGCACCAGGCCTCTT
  CACCTACCTCCAGCCCTGCTACTTCTTTCCCTCCTCCCCCTTCCTCTGGAGCATCCTTCCAGCATGGCGG
  ACCAGGAGCTCCACCATCATCTTCAGCTTATGCACTGCCTCCTGGAACAACAGGTCCTCAGAATGGTTGG
  AATGACCCTCCAGCTTTGAACAGAGTACCCAAAAAGAAGAAGATGCCTGAAAACTTCATGCCTCCTGTTC
  CCATCACATCACCAATCATGAACCCGTTGGGTGACCCCCAGTCACAAATGCTGCAGCAACAGCCTTCAGC
  TCCAGTACCACTGTCAAGCCAGTCTTCATTCCCACAGCCACATCTTCCAGGTGGCCAGCCCTTCCATGGC
  GTACAGCAACCTCTTGGTCAAACAGGCATGCCACCATCTTTTTCAAAGCCCAATATTGAAGGTGCCCCAG
  GGGCTCCTATTGGAAATACCTTCCAGCATGTGCAGTCTTTGCCAACAAAAAAAATTACCAAGAAACCTAT
  TCCAGATGAGCACCTCATTCTAAAGACCACATTTGAGGATCTTATTCAGCGCTGCCTTTCTTCAGCAACA
  GACCCTCAAACCAAGAGGAAGCTAGATGATGCCAGCAAACGTTTGGAGTTTCTGTATGATAAACTTAGGG
  AACAGACACTTTCACCAACAATCACCAGTGGTTTACACAACATTGCAAGGAGCATTGAAACTCGAAACTA
  CTCAGAAGGATTGACCATGCATACCCACATAGTTAGCACCAGCAACTTCAGTGAGACCTCTGCTTTCATG
  CCAGTTCTCAAAGTTGTTCTCACCCAGGCCAATAAGCTGGGTGTCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001077208
• ORF Size: 3618 bp
• Insert Size: 0
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001077208.3, NP_001070676.1
• RefSeq Size: 4411
• RefSeq ORF: 3618
• Locus ID: 22872
• Gene Summary: The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 5. The encoded isoform (3) has the same N- and C-termini, but is shorter than isoform 1. Both variants 3 and 9 encode the same isoform (3).