KDM6B (NM_001080424) Human Untagged Clone

CAT#: SC315893

KDM6B (untagged)-Human lysine (K)-specific demethylase 6B (KDM6B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KDM6B
• Synonyms: JMJD3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001080424, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCATCGGGCAGTGGACCCTCCAGGGGCCCGCGCTGCACGGGAAGCCTTTGCCCTTGGGGGCCTGAGCT
  GTGCTGGGGCCTGGAGCTCCTGCCCGCCTCATCCCCCTCCTCGTAGCGCATGGCTGCCTGGAGGCAGATG
  CTCAGCCAGCATTGGGCAGCCCCCGCTTCCTGCTCCCCTACCCCCTTCACATGGCAGTAGTTCTGGGCAC
  CCCAGCAAACCATATTATGCTCCAGGGGCGCCCACTCCAAGACCCCTCCATGGGAAGCTGGAATCCCTGC
  ATGGCTGTGTGCAGGCATTGCTCCGGGAGCCAGCCCAGCCAGGGCTTTGGGAACAGCTTGGGCAACTGTA
  CGAGTCAGAGCACGATAGTGAGGAGGCCACACGCTGCTACCACAGCGCCCTTCGATACGGAGGAAGCTTC
  GCTGAGCTGGGGCCCCGCATTGGCCGACTGCAGCAGGCCCAGCTCTGGAACTTTCATACTGGCTCCTGCC
  AGCACCGAGCCAAGGTCCTGCCCCCACTGGAGCAAGTGTGGAACTTGCTACACCTTGAGCACAAACGGAA
  CTATGGAGCCAAGCGGGGAGGTCCCCCGGTGAAGCGAGCTGCTGAACCCCCAGTGGTGCAGCCTGTGCCT
  CCTGCAGCACTCTCAGGCCCCTCAGGGGAGGAGGGCCTCAGCCCTGGAGGCAAGCGAAGGAGAGGCTGCA
  ACTCTGAACAGACTGGCCTTCCCCCAGGGCTGCCACTGCCTCCACCACCATTACCACCACCACCACCACC
  ACCACCACCACCACCACCACCCCTGCCTGGCCTGGCTACCAGCCCCCCATTTCAGCTAACCAAGCCAGGG
  CTGTGGAGTACCCTGCATGGAGATGCCTGGGGCCCAGAGCGCAAGGGTTCAGCACCCCCAGAGCGCCAGG
  AGCAGCGGCACTCGCTGCCTCACCCATATCCATACCCAGCTCCAGCGTACACCGCGCACCCCCCTGGCCA
  CCGGCTGGTCCCGGCTGCTCCCCCAGGCCCAGGCCCCCGCCCCCCAGGAGCAGAGAGCCATGGCTGCCTG
  CCTGCCACCCGTCCCCCCGGAAGTGACCTTAGAGAGAGCAGAGTTCAGAGGTCGCGGATGGACTCCAGCG
  TTTCACCAGCAGCAACCACCGCCTGCGTGCCTTACGCCCCTTCCCGGCCCCCTGGCCTCCCCGGCACCAC
  CACCAGCAGCAGCAGTAGCAGCAGCAGCAACACTGGTCTCCGGGGCGTGGAGCCGAACCCAGGCATTCCC
  GGCGCTGACCATTACCAAACTCCCGCGCTGGAGGTCTCTCACCATGGCCGCCTGGGGCCCTCGGCACACA
  GCAGTCGGAAACCGTTCTTGGGGGCTCCCGCTGCCACTCCCCACCTATCCCTGCCACCTGGACCTTCCTC
  ACCCCCTCCACCCCCCTGTCCCCGCCTCTTACGCCCCCCACCACCCCCTGCCTGGTTGAAGGGTCCGGCC
  TGCCGGGCAGCCCGAGAGGATGGAGAGATCTTAGAAGAGCTCTTCTTTGGGACTGAGGGACCCCCCCGCC
  CTGCCCCACCACCCCTCCCCCATCGCGAGGGCTTCTTGGGGCCTCCGGCCTCCCGCTTTTCTGTGGGCAC
  TCAGGATTCTCACACCCCTCCCACTCCCCCAACCCCAACCACCAGCAGTAGCAACAGCAACAGTGGCAGC
  CACAGCAGCAGCCCTGCTGGGCCTGTGTCCTTTCCCCCACCACCCTATCTGGCCAGAAGTATAGACCCCC
  TTCCCCGGCCTCCCAGCCCAGCACAGAACCCCCAGGACCCACCTCTTGTACCCCTGACTCTTGCCCTGCC
  TCCAGCCCCTCCTTCCTCCTGCCACCAAAATACCTCAGGAAGCTTCAGGCGCCCGGAGAGCCCCCGGCCC
  AGGGTCTCCTTCCCAAAGACCCCCGAGGTGGGGCCGGGGCCACCCCCAGGCCCCCTGAGTAAAGCCCCCC
  AGCCTGTGCCGCCCGGGGTTGGGGAGCTGCCTGCCCGAGGCCCTCGACTCTTTGATTTTCCCCCCACTCC
  GCTGGAGGACCAGTTTGAGGAGCCAGCCGAATTCAAGATCCTACCTGATGGGCTGGCCAACATCATGAAG
  ATGCTGGACGAATCCATTCGCAAGGAAGAGGAACAGCAACAACACGAAGCAGGCGTGGCCCCCCAACCCC
  CGCTGAAGGAGCCCTTTGCATCTCTGCAGTCTCCTTTCCCCACCGACACAGCCCCCACCACTACTGCTCC
  TGCTGTCGCCGTCACCACCACCACCACCACCACCACCACCACCACGGCCACCCAGGAAGAGGAGAAGAAG
  CCACCACCAGCCCTACCACCACCACCGCCTCTAGCCAAGTTCCCTCCACCCTCTCAGCCACAGCCACCAC
  CACCCCCACCCCCCAGCCCGGCCAGCCTGCTCAAATCCTTGGCCTCCGTGCTGGAGGGACAAAAGTACTG
  TTATCGGGGGACTGGAGCAGCTGTTTCCACCCGGCCTGGGCCCTTGCCCACCACTCAGTATTCCCCTGGC
  CCCCCATCAGGTGCTACCGCCCTGCCGCCCACCTCAGCGGCCCCTAGCGCCCAGGGCTCCCCACAGCCCT
  CTGCTTCCTCGTCATCTCAGTTCTCTACCTCAGGCGGGCCCTGGGCCCGGGAGCGCAGGGCGGGCGAAGA
  GCCAGTCCCGGGCCCCATGACCCCCACCCAACCGCCCCCACCCCTATCTCTGCCCCCTGCTCGCTCTGAG
  TCTGAGGTGCTAGAAGAGATCAGCCGGGCTTGCGAGACCCTTGTGGAGCGGGTGGGCCGGAGTGCCACTG
  ACCCAGCCGACCCAGTGGACACAGCAGAGCCAGCGGACAGTGGGACTGAGCGACTGCTGCCCCCCGCACA
  GGCCAAGGAGGAGGCTGGCGGGGTGGCGGCAGTGTCAGGCAGCTGTAAGCGGCGACAGAAGGAGCATCAG
  AAGGAGCATCGGCGGCACAGGCGGGCCTGTAAGGACAGTGTGGGTCGTCGGCCCCGTGAGGGCAGGGCAA
  AGGCCAAGGCCAAGGTCCCCAAAGAAAAGAGCCGCCGGGTGCTGGGGAACCTGGACCTGCAGAGCGAGGA
  GATCCAGGGTCGTGAGAAGTCCCGGCCCGATCTTGGCGGGGCCTCCAAGGCCAAGCCACCCACAGCTCCA
  GCCCCTCCATCAGCTCCTGCACCTTCTGCCCAGCCCACACCCCCGTCAGCCTCTGTCCCTGGAAAGAAGG
  CTCGGGAGGAAGCCCCAGGGCCACCGGGTGTCAGCCGGGCCGACATGCTGAAGCTGCGCTCACTTAGTGA
  GGGGCCCCCCAAGGAGCTGAAGATCCGGCTCATCAAGGTAGAGAGTGGTGACAAGGAGACCTTTATCGCC
  TCTGAGGTGGAAGAGCGGCGGCTGCGCATGGCAGACCTCACCATCAGCCACTGTGCTGCTGACGTCGTGC
  GCGCCAGCAGGAATGCCAAGGTGAAAGGGAAGTTTCGAGAGTCCTACCTTTCCCCTGCCCAGTCTGTGAA
  ACCGAAGATCAACACTGAGGAGAAGCTGCCCCGGGAAAAACTCAACCCCCCTACACCCAGCATCTATCTG
  GAGAGCAAACGGGATGCCTTCTCACCTGTCCTGCTGCAGTTCTGTACAGACCCTCGAAATCCCATCACAG
  TGATCCGGGGCCTGGCGGGCTCCCTGCGGCTCAACTTGGGCCTCTTCTCCACCAAGACCCTGGTGGAAGC
  GAGTGGCGAACACACCGTGGAAGTTCGCACCCAGGTGCAGCAGCCCTCAGATGAGAACTGGGATCTGACA
  GGCACTCGGCAGATCTGGCCTTGTGAGAGCTCCCGTTCCCACACCACCATTGCCAAGTACGCACAGTACC
  AGGCCTCATCCTTCCAGGAGTCTCTGCAGGAGGAGAAGGAGAGTGAGGATGAGGAGTCAGAGGAGCCAGA
  CAGCACCACTGGAACCCCTCCTAGCAGCGCACCAGACCCGAAGAACCATCACATCATCAAGTTTGGCACC
  AACATCGACTTGTCTGATGCTAAGCGGTGGAAGCCCCAGCTGCAGGAGCTGCTGAAGCTGCCCGCCTTCA
  TGCGGGTAACATCCACGGGCAACATGCTGAGCCACGTGGGCCACACCATCCTGGGCATGAACACGGTGCA
  GCTGTACATGAAGGTGCCCGGCAGCCGAACGCCAGGCCACCAGGAGAATAACAACTTCTGCTCCGTCAAC
  ATCAACATTGGCCCAGGCGACTGCGAGTGGTTCGCGGTGCACGAGCACTACTGGGAGACCATCAGCGCTT
  TCTGTGATCGGCACGGCGTGGACTACTTGACGGGTTCCTGGTGGCCAATCCTGGATGATCTCTATGCATC
  CAATATTCCTGTGTACCGCTTCGTGCAGCGACCCGGAGACCTCGTGTGGATTAATGCGGGGACTGTGCAC
  TGGGTGCAGGCCACCGGCTGGTGCAACAACATTGCCTGGAACGTGGGGCCCCTCACCGCCTATCAGTACC
  AGCTGGCCCTGGAACGATACGAGTGGAATGAGGTGAAGAACGTCAAATCCATCGTGCCCATGATTCACGT
  GTCATGGAACGTGGCTCGCACGGTCAAAATCAGCGACCCCGACTTGTTCAAGATGATCAAGTTCTGCCTG
  CTGCAGTCCATGAAGCACTGCCAGGTGCAACGCGAGAGCCTGGTGCGGGCAGGGAAGAAAATCGCTTACC
  AGGGCCGTGTCAAGGACGAGCCAGCCTACTACTGCAACGAGTGCGATGTGGAGGTGTTTAACATCCTGTT
  CGTGACAAGTGAGAATGGCAGCCGCAACACGTACCTGGTACACTGCGAGGGCTGTGCCCGGCGCCGCAGC
  GCAGGCCTGCAGGGCGTGGTGGTGCTGGAGCAGTACCGCACTGAGGAGCTGGCTCAGGCCTACGACGCCT
  TCACGCTGGTGAGGGCCCGGCGGGCGCGCGGGCAGCGGAGGAGGGCACTGGGGCAGGCTGCAGGGACGGG
  CTTCGGGAGCCCGGCCGCGCCTTTCCCTGAGCCCCCGCCGGCTTTCTCCCCCCAGGCCCCAGCCAGCACG
  TCGCGATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001080424
• ORF Size: 5049 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001080424.1, NP_001073893.1
• RefSeq Size: 6704
• RefSeq ORF: 5049
• Locus ID: 23135
• Gene Summary: The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2017]
  Transcript Variant: This variant (1) retains an intron in the 3' coding region, compared to variant 2, and encodes a longer protein (isoform 1), compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.