PORCN (NM_022825) Human Untagged Clone

CAT#: SC316376

PORCN (untagged)-Human porcupine homolog (Drosophila) (PORCN), transcript variant A

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PORCN
• Synonyms: DHOF; FODH; MG61; PORC; PPN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022825, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCACCTTTAGCCGCCAGGAATTTTTCCAGCAGCTACTGCAAGGCTGTCTCCTGCCTACTGCCCAGC
  AGGGCCTTGACCAGATCTGGCTGCTCCTTGCCATCTGCCTCGCCTGCCGCCTCCTCTGGAGGCTCGGGTT
  GCCATCCTACCTGAAGCATGCAAGCACCGTGGCAGGCGGGTTCTTCAGCCTCTACCACTTCTTCCAGCTG
  CACATGGTTTGGGTCGTGCTGCTCAGCCTCCTGTGCTACCTCGTGCTGTTCCTCTGCCGACATTCCTCCC
  ATCGAGGCGTCTTCCTATCCGTCACCATCCTCATCTACCTACTCATGGGTGAGATGCACATGGTAGACAC
  CGTGACATGGCACAAGATGCGAGGGGCACAGATGATTGTGGCCATGAAGGCAGTGTCTCTGGGCTTCGAC
  CTGGACCGGGGCGAGGTGGGTACGGTGCCCTCGCCAGTGGAGTTCATGGGCTACCTCTACTTCGTGGGCA
  CCATCGTCTTCGGGCCCTGGATATCCTTCCACAGCTACCTACAAGCTGTCCAAGGCCGCCCACTGAGCTG
  CCGGTGGCTGCAGAAGGTGGCCCGGAGCCTGGCACTGGCCCTGCTGTGCCTTGTGCTGTCCACTTGCGTG
  GGCCCCTACCTCTTCCCGTACTTCATCCCCCTCAACGGTGACCGCCTCCTTCGCAAGTGGCTGCGAGCCT
  ACGAGAGTGCTGTCTCCTTCCACTTCAGCAACTATTTTGTGGGCTTTCTTTCCGAGGCCACGGCCACGTT
  GGCGGGGGCTGGCTTTACCGAGGAGAAGGATCACCTGGAATGGGACCTGACGGTGTCCAAGCCACTGAAT
  GTGGAGCTGCCTCGGTCAATGGTGGAAGTTGTCACAAGCTGGAACCTGCCCATGTCTTATTGGCTAAATA
  ACTATGTTTTCAAGAATGCTCTCCGCCTGGGGACCTTCTCGGCTGTGCTGGTCACCTATGCAGCCAGCGC
  CCTCCTACATGGCTTCAGTTTCCACCTGGCTGCGGTCCTGCTGTCCCTGGCTTTTATCACTTACGTGGAG
  CATGTCCTCCGGAAGCGCCTGGCTCGGATCCTCAGTGCCTGTGTCTTGTCAAAGCGGTGCCCGCCAGACT
  GTTCGCACCAGCATCGCTTGGGCCTGGGGGTGCGAGCCTTAAACTTGCTCTTTGGAGCTCTGGCCATCTT
  CCACCTGGCCTACCTGGGCTCCCTGTTTGATGTCGATGTGGATGACACCACAGAGGAGCAGGGCTACGGC
  ATGGCATACACTGTCCACAAGTGGTCAGAGCTCAGCTGGGCCAGTCACTGGGTCACTTTTGGATGCTGGA
  TCTTCTACCGTCTCATAGGCTGA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_022825
• ORF Size: 1353 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_022825.2, NP_073736.2
• RefSeq Size: 1849
• RefSeq ORF: 1353
• Locus ID: 64840
• Domains: MBOAT
• Protein Families: Transmembrane
• Protein Pathways: Wnt signaling pathway
• Gene Summary: This gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the porcupine family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Disruption of this gene is associated with focal dermal hypoplasia, and the encoded protein has been implicated in cancer. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (A) lacks two in-frame exons in the central coding region, compared to variant D. The resulting isoform (A) is shorter, compared to isoform D.