TAF4B (NM_005640) Human Untagged Clone

CAT#: SC316429

TAF4B (untagged)-Human TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa (TAF4B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAF4B
• Synonyms: SPGF13; TAF2C2; TAFII105
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005640, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCGCCGGCCTCACCGAACCCGCCGGCGCCGCTCCCCCGGCTGCTGTGAGCGCCTCGGGGACCGTGA
  CCATGGCCCCGGCCGGGGCGCTGCCGGTGCGGGTGGAGAGCACTCCGGTGGCCCTGGGCGCCGTGACTAA
  GGCTCCTGTCAGCGTCTGCGTGGAGCCCACGGCGTCCCAGCCCCTGCGGTCCCCCGTGGGGACCCTGGTG
  ACCAAAGTGGCTCCGGTCAGCGCCCCTCCTAAAGTCAGCAGCGGCCCTAGGCTGCCTGCTCCTCAGATAG
  TCGCCGTGAAAGCCCCCAACACCACGACAATCCAGTTTCCTGCTAATTTGCAGCTTCCTCCAGGAACCGT
  TTTGATTAAAAGTAACAGTGGTCCGTTGATGTTGGTATCTCCTCAGCAAACTGTAACAAGAGCCGAGACC
  ACAAGTAACATAACCTCAAGGCCAGCAGTACCAGCGAATCCTCAAACAGTCAAAATCTGTACAGTGCCGA
  ACTCTAGCTCACAATTAATCAAGAAAGTGGCAGTGACACCTGTTAAAAAATTGGCACAAATAGGAACTAC
  TGTGGTAACCACTGTTCCGAAGCCTTCCTCAGTACAATCTGTGGCTGTGCCAACCAGTGTCGTCACAGTT
  ACTCCTGGAAAGCCATTGAATACTGTAACTACCCTGAAGCCTTCAAGTTTGGGAGCATCATCCACTCCTT
  CAAATGAGCCCAATCTTAAAGCAGAGAACTCAGCAGCTGTTCAGATTAATCTTTCTCCGACAATGCTAGA
  AAATGTGAAGAAATGCAAGAACTTCCTTGCAATGTTAATAAAACTAGCATGTAGTGGATCACAGTCCCCA
  GAAATGGGGCAAAATGTGAAGAAGCTGGTGGAACAACTTTTGGATGCAAAAATCGAAGCAGAAGAATTTA
  CTAGGAAACTGTATGTTGAACTCAAGTCTTCACCTCAGCCTCACCTGGTTCCTTTTCTTAAGAAAAGCGT
  GGTTGCCTTACGACAACTTCTGCCTAACTCCCAGAGCTTCATCCAGCAATGTGTTCAGCAGACTTCTAGT
  GACATGGTCATTGCTACCTGTACTACAACAGTAACAACTTCTCCTGTGGTGACAACTACAGTGTCCTCAA
  GCCAGTCTGAAAAGTCAATTATTGTTTCTGGAGCAACAGCACCCAGAACTGTGTCAGTGCAAACTTTGAA
  CCCACTTGCTGGTCCAGTGGGAGCAAAAGCTGGAGTTGTGACACTTCATTCTGTGGGCCCAACTGCTGCA
  ACAGGAGGAACAACAGCTGGAACTGGTTTGCTTCAGACTTCAAAACCACTTGTGACATCTGTGGCAAACA
  CAGTGACCACGGTCTCACTGCAACCTGAAAAGCCAGTTGTCTCTGGAACAGCAGTAACACTGTCCCTTCC
  AGCAGTAACTTTTGGAGAAACTTCAGGTGCAGCTATTTGTCTTCCATCTGTGAAACCTGTTGTTTCTTCT
  GCTGGGACCACATCTGACAAGCCTGTTATTGGGACTCCAGTTCAAATCAAACTTGCCCAGCCGGGCCCTG
  TCCTTTCACAACCAGCTGGGATTCCACAGGCAGTTCAAGTCAAGCAACTAGTAGTTCAGCAGCCTTCAGG
  AGGCAATGAAAAACAAGTGACCACAATTTCACATTCCTCAACATTGACCATTCAGAAATGTGGACAGAAG
  ACGATGCCAGTGAACACCATAATACCTACTAGTCAGTTTCCTCCAGCTTCCATTCTAAAGCAAATTACTC
  TGCCTGGAAATAAAATTCTGTCACTTCAAGCATCTCCTACTCAGAAAAATAGAATAAAAGAGAATGTAAC
  ATCATGCTTCCGAGATGAGGATGACATCAATGATGTGACTTCTATGGCAGGGGTCAACCTTAATGAAGAA
  AATGCCTGCATCTTAGCAACAAACTCTGAATTGGTTGGCACACTCATTCAGTCATGTAAAGATGAACCAT
  TTCTTTTTATTGGAGCTCTACAAAAGAGAATTTTAGACATTGGTAAAAAGCATGACATTACAGAACTTAA
  CTCTGATGCTGTGAACTTGATCTCCCAAGCAACACAGGAACGACTACGAGGCCTTCTAGAAAAACTGACT
  GCAATTGCTCAGCATCGAATGACTACTTACAAGGCAAGTGAAAATTACATCCTGTGTAGTGATACCAGGT
  CACAGCTCAAATTTCTTGAAAAGCTGGATCAATTGGAGAAGCAGAGAAAGGATTTGGAAGAAAGAGAAAT
  GTTACTTAAGGCAGCCAAGAGTCGTTCTAATAAAGAAGATCCAGAACAGCTGAGATTAAAGCAGAAAGCC
  AAAGAGTTACAGCAATTGGAACTTGCACAGATACAGCATAGAGACGCTAATCTCACAGCTCTTGCAGCTA
  TTGGACCAAGGAAGAAGAGACCACTAGAATCTGGAATTGAGGGCTTAAAAGACAACCTTCTTGCTTCTGG
  GACATCCAGCCTGACAGCCACCAAACAGTTGCATCGTCCAAGAATCACGAGAATCTGCCTCAGGGACTTG
  ATATTTTGTATGGAACAGGAACGGGAGATGAAGTATTCTCGAGCTCTATACCTGGCCCTTCTGAAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005640
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005640.2, NP_005631.1
• RefSeq Size: 4339 bp
• RefSeq ORF: 2589 bp
• Locus ID: 6875
• Cytogenetics: 18q11.2
• Protein Pathways: Basal transcription factors, Huntington's disease
• Gene Summary: 'TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.