Rubicon (RUBCN) (NM_014687) Human Untagged Clone

CAT#: SC316580

KIAA0226 (untagged)-Human KIAA0226 (KIAA0226), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RUBCN
• Synonyms: KIAA0226; RUBICON; SCAR15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014687, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGGCCGGAGGGCGCGGGAATGGAGCTCGGAGGCGGCGAGGAGCGCCTGCCTGAGGAGAGCAGGAGGG
  AGCACTGGCAGTTGCTGGGTAATTTGAAGACGACGGTGGAGGGTTTGGTATCAACCAACAGCCCCAACGT
  CTGGTCTAAGTATGGTGGCTTGGAGCGGCTTTGCAGGGACATGCAGAGCATCCTCTATCACGGGCTTATC
  CGTGACCAGGCGTGCCGCCGCCAGACGGATTACTGGCAGTTCGTGAAAGACATCCGGTGGCTCAGTCCCC
  ACTCAGCCCTTCACGTGGAGAAGTTCATCAGCGTGCACGAGAACGACCAGAGCAGTGCTGATGGTGCCAG
  TGAACGTGCTGTTGCCGAGCTGTGGCTGCAGCACAGCCTGCAGTACCACTGCCTCTCAGCCCAGCTCCGG
  CCCCTGCTCGGGGATAGACAGTATATCAGAAAATTCTACACAGATGCTGCCTTCCTGCTAAGTGACGCTC
  ATGTCACGGCCATGCTGCAGTGCCTGGAAGCAGTGGAACAGAACAACCCCCGCCTCCTGGCTCAGATCGA
  TGCGTCCATGTTTGCCAGAAAGCACGAGAGCCCGCTCCTGGTGACAAAGAGCCAGAGCCTGACAGCCCTG
  CCCAGTTCCACATACACCCCTCCAAACAGCTATGCTCAGCATTCCTACTTTGGGTCCTTCTCTAGCCTCC
  ACCAATCCGTGCCCAACAATGGCTCAGAGAGAAGATCTACTTCCTTTCCACTCTCTGGCCCTCCCCGGAA
  ACCTCAAGAAAGCAGAGGGCACGTCTCACCAGCAGAGGATCAAACCATCCAAGCCCCCCCAGTTTCAGTC
  TCTGCACTAGCCAGGGATTCCCCTTTGACCCCAAATGAAATGAGCTCCAGTACTCTGACCAGCCCCATAG
  AGGCATCCTGGGTCAGCAGCCAGAATGATTCCCCAGGTGATGCCAGTGAGGGGCCTGAGTACCTGGCCAT
  TGGCAACTTGGACCCCCGAGGCCGGACTGCCAGCTGTCAGAGTCACAGCAGCAATGCCGAGAGCAGCAGT
  TCCAATTTGTTCTCCTCCAGCAGCTCCCAGAAGCCAGATTCTGCTGCCTCTTCCTTAGGGGACCAGGAAG
  GAGGTGGGGAGAGCCAGCTGTCCAGTGTCCTCCGCAGGTCCAGCTTCTCAGAGGGGCAGACACTCACTGT
  CACCAGTGGGGCAAAGAAAAGCCACATTCGCTCCCATTCGGATACCAGCATTGCCTCCAGGGGAGCTCCA
  GAATCCTGCAATGATAAGGCGAAGTTGAGAGGCCCTTTGCCCTACTCTGGTCAAAGCAGTGAAGTCAGCA
  CACCCAGCTCTCTGTACATGGAATATGAAGGTGGTCGGTACCTGTGCTCAGGGGAAGGCATGTTCCGAAG
  ACCATCAGAAGGACAGTCCCTCATCAGCTACCTCTCTGAGCAAGACTTCGGCAGCTGTGCCGACCTGGAA
  AAGGAGAATGCCCACTTCAGCATCTCAGAGTCCTTAATTGCTGCCATCGAGCTAATGAAGTGCAACATGA
  TGAGCCAGTGCCTAGAGGAGGAGGAAGTGGAAGAGGAAGACAGTGATAGAGAGATCCAGGAGCTGAAGCA
  GAAGATCCGCCTTCGGCGCCAGCAAATCCGCACCAAGAACCTGCTCCCCATGTACCAGGAGGCTGAGCAC
  GGAAGCTTTCGGGTCACCTCCAGCAGCTCCCAGTTCAGCTCACGTGATTCGGCACAGCTCTCTGACTCTG
  GCTCTGCTGATGAGGTTGATGAATTTGAAATCCAAGATGCTGACATCAGAAGGAACACAGCCTCAAGCAG
  CAAATCCTTCGTTTCCTCCCAGTCCTTCTCCCACTGCTTCCTGCACTCCACGTCTGCTGAGGCGGTGGCC
  ATGGGGCTCCTGAAGCAGTTTGAGGGGATGCAGCTTCCAGCCGCCTCGGAGCTGGAGTGGCTTGTCCCGG
  AGCATGATGCCCCTCAGAAGCTCCTGCCCATTCCTGACTCACTGCCCATCTCACCGGATGACGGGCAGCA
  CGCTGACATCTACAAGCTGCGGATTCGTGTTCGTGGCAACTTGGAGTGGGCCCCGCCCCGGCCTCAGATA
  ATTTTTAATGTTCATCCAGCCCCAACGAGGAAAATTGCCGTGGCCAAGCAGAATTACCGCTGTGCAGGAT
  GTGGCATCCGGACTGACCCTGATTACATCAAGCGACTGCGGTACTGTGAGTACCTGGGCAAGTACTTCTG
  CCAGTGCTGCCACGAGAATGCCCAGATGGCCATCCCCAGCCGGGTTCTGCGCAAGTGGGACTTCAGCAAG
  TACTACGTCAGCAACTTCTCCAAGGACCTGCTCATTAAGATCTGGAATGATCCTCTCTTCAACGTGCAGG
  ACATAAACAGTGCCCTCTATAGGAAGGTCAAGCTGCTCAATCAAGTCCGGCTGCTGCGGGTCCAGCTGTG
  TCACATGAAGAACATGTTCAAGACTTGCCGACTGGCCAAGGAGCTTCTGGATTCCTTTGACACAGTCCCA
  GGCCACCTGACAGAGGACCTCCACCTGTACTCACTGAATGACCTGACTGCGACCAGGAAGGGGGAGCTGG
  GGCCCCGGCTTGCTGAGCTCACCAGGGCAGGGGCTACCCATGTGGAGAGATGCATGCTCTGCCAAGCCAA
  AGGCTTCATCTGTGAGTTCTGTCAGAATGAGGATGACATCATCTTTCCCTTTGAGCTCCATAAGTGCCGG
  ACCTGTGAAGAGTGTAAAGCGTGTTACCATAAAGCCTGCTTCAAGTCTGGAAGCTGTCCGCGCTGCGAGC
  GGCTGCAGGCCCGGCGGGAGGCACTGGCCAGGCAGAGCCTGGAGTCTTACCTGTCAGACTACGAGGAGGA
  GCCCGCGGAAGCGCTGGCCCTGGAAGCCGCCGTCCTGGAGGCCACCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014687
• ORF Size: 2919 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_014687.2, NP_055502.1
• RefSeq Size: 6762
• RefSeq ORF: 2919
• Locus ID: 9711
• Gene Summary: The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (2) represents a shorter transcript, but encodes the longer isoform (2).