EIF2A (NM_032025) Human Untagged Clone

CAT#: SC316786

EIF2A (untagged)-Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF2A
• Synonyms: CDA02; EIF-2A; MST089; MSTP004; MSTP089
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_032025 edited
  ATGGCGCCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAAT
  GGACCACCACATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAA
  GTCTGTATCTTTAGTAAGGATGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAAT
  ATTATCAGTGTCACTAACAAGGGACTACTGCACTCCTTCGACCTCCTGAAGGCAGTTTGC
  CTTGAATTCTCACCCAAAAATACTGTCCTGGCAACGTGGCAGCCTTACAGTACTTCTAAA
  GATGGCACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGGGACATGTTTG
  AAATCTTTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACT
  CTTTGTGCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACA
  ATTGCAAATAAATTGCATTTGCAAAAAATTAATGATTTTGTATTATCACCTGGACCCCAA
  CCATACAAGGTGGCTGTCTATGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTA
  TATCAGTACCCCAACTTTGCTGGACCTCATGCAGCTTTAGCTAATAAAAGTTTCTTTAAG
  GCAGATAAAGTTACAATGCTGTGGAATAAAAAAGCTACTGCTGTGTTGGTAATAGCTAGC
  ACAGATGTTGACAAGACAGGAGCTTCCTACTATGGAGAACAAACTCTACACTACATTGCA
  ACAAATGGAGAAAGTGCTGTAGTGCAATTACCAAAAAATGGCCCCATTTATGATGTAGTT
  TGGAATTCTAGTTCTACTGAGTTTTGTGCTGTATATGGTTTTATGCCTGCCAAAGCGACA
  ATTTTCAACTTGAAATGTGATCCTGTATTTGACTTTGGAACTGGTCCTCGTAATGCAGCC
  TACTATAGCCCTCATGGACATATATTAGTATTAGCTGGATTTGGAAATCTGAGGGGACAA
  ATGGAAGTGTGGGATGTGAAAAACTACAAACTTATTTCTAAACCGGTGGCTTCTGATTCT
  ACATATTTTGCTTGGTGCCCGGATGGTGAGCATATTTTAACAGCTACATGTGCTCCCAGG
  TTACGGGTTAATAATGGATACAAAATTTGGCATTATACTGGCTCTATCTTGCACAAGTAT
  GATGTGCCATCAAATGCAGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATA
  TTTCCAGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCAATGAGGAACCT
  AAAGTTGCAACAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCACCAATTCCAAA
  TTGCATGAAGAGGAACCACCTCAGAATATGAAACCACAATCAGGAAACGATAAGCCATTA
  TCAAAAACAGCTCTTAAAAATCAAAGGAAGCATGAAGCTAAGAAAGCTGCAAAGCAGGAA
  GCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTGCCCCACAGAGCACACCACGA
  AACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAATCAAGAACCTA
  AAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTGGAAAACAGCTA
  GAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGGAGCTGGAAGAT
  TTGGAATTGGGTATTTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032025 unedited
  ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGACAACATGGCG
  CCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAATGGACCA
  CCACATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAAGTCTGT
  ATCTTTAGTAAGGATGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAATATTATC
  AGTGTCACTAACAAGGGACTACTGCACTCCTTCGACCTCCTGAAGGCAGTTTGCCTTGAA
  TTCTCACCCAAAAATACTGTCCTGGCAACGTGGCAGCCTTACAGTACTTCTAAAGATGGC
  ACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGGGACATGTTTGAAATCT
  TTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACTCTTTGT
  GCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACAATTGCA
  AATAAATTGCATTTGCAAAAAATTAATGATTTTGTATTATCACCTGGACCCCAACCATAC
  AAGGTGGCTGTCTATGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTATATCAG
  TACCCCAACTTTGCTGGACCTCATGCAGCTTTAGCTAATAAAAGTTTCTTTAAGGCAGAT
  AAAGTTACAATGCTGTGGAATAAAAAGCTACTGCTGTGTTGGGTATAGCTAGCACAGATG
  TTGACAGACAGGAGCTTCCTACTTGGAGAACANACTCTACACTACATTGCACAATGGNAG
  AAGTGGCTGTAGTGCCAATACCAAAAATGGCCCATTTATGGATGTAGTT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_032025 unedited
  CTTTTTGTGGATTTTCCGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCA
  ATGAGGAACCTAAAGTTGCAACAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCA
  CCAATTCCAAATTGCATGAAGAGGAACCACCTCAGAATATGAAACCACAATCAGGAAACG
  ATAAGCCATTATCAAAAACAGCTCTTAAAAATCAAAGGAAGCATGAAGCTAAGAAAGCTG
  CAAAGCAGGAAGCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTGCCCCACAGA
  GCACACCACGAAACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAA
  TCAAGAACCTAAAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTG
  GAAAACAGCTAGAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGG
  AGCTGGAAGATTTGGAATTGGGTATTTAAAGATTCACGGAAAGCAAGTTGATGACCAGAA
  ATCAGTGCAAACACATCTTCTGTTAAACCCATTGGTATACACAGAATATTCCTGTGCCCA
  CACTTAATGTCAATCTATAATTTTAACCATTTATCCAAGATTCTACTAAGTGTAAAATTA
  TTTAATAATGTCTATTAAATTGATATTTATATCTTGCATCCTATATCATGTCAATATGTG
  ATATAGAAAAGAGATACGTGAATTTTTTAGCTAAGCTTGACAGATTGAAAGACAAGTGTC
  ATTTTTTTTTGTAGAGGGTGATATATACCATGTAAATGAATAAAGACATTTTAAATTTAC
  AACAAAACAAAAACACTCGACTTCTAGAATTGCGCCCGCGGTCAATAGCTTGTTTCCTGA
  ACAAAATCCCGGGTGGCATCCCTGGTGACCCCTCCCCAGTGGCTCTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032025
• ORF Size: 1758 bp
• Insert Size: 3800
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.NA

Reference Data

• RefSeq: NM_032025.3, NP_114414.2
• RefSeq Size: 3894
• RefSeq ORF: 1758
• Locus ID: 83939
• Gene Summary: This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.