EVI1 (MECOM) (NM_001105077) Human Untagged Clone

CAT#: SC317159

MECOM (untagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MECOM
• Synonyms: AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1; PRDM3; RUSAT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001105077, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATCTTAGACGAATTTTACAATGTGAAGTTCTGCATAGATGCCAGTCAACCAGATGTTGGAAGCTGGC
  TCAAGTACATTAGATTCGCTGGCTGTTATGATCAGCACAACCTTGTTGCATGCCAGATAAATGATCAGAT
  ATTCTATAGAGTAGTTGCAGACATTGCGCCGGGAGAGGAGCTTCTGCTGTTCATGAAGAGCGAAGACTAT
  CCCCATGAAACTATGGCGCCGGATATCCACGAAGAACGGCAATATCGCTGCGAAGACTGTGACCAGCTCT
  TTGAATCTAAGGCTGAACTAGCAGATCACCAAAAGTTTCCATGCAGTACTCCTCACTCAGCATTTTCAAT
  GGTTGAAGAGGACTTTCAGCAAAAACTCGAAAGCGAGAATGATCTCCAAGAGATACACACGATCCAGGAG
  TGTAAGGAATGTGACCAAGTTTTTCCTGATTTGCAAAGCCTGGAGAAACACATGCTGTCACATACTGAAG
  AGAGGGAATACAAGTGTGATCAGTGTCCCAAGGCATTTAACTGGAAGTCCAATTTAATTCGCCACCAGAT
  GTCACATGACAGTGGAAAGCACTATGAATGTGAAAACTGTGCCAAGCAGGTTTTCACGGACCCTAGCAAC
  CTTCAGCGGCACATTCGCTCTCAGCATGTCGGTGCCCGGGCCCATGCATGCCCGGAGTGTGGCAAAACGT
  TTGCCACTTCGTCGGGCCTCAAACAACACAAGCACATCCACAGCAGTGTGAAGCCCTTTATCTGTGAGGT
  CTGCCATAAATCCTATACTCAGTTTTCAAACCTTTGCCGTCATAAGCGCATGCATGCTGATTGCAGAACC
  CAAATCAAGTGCAAAGACTGTGGACAAATGTTCAGCACTACGTCTTCCTTAAATAAACACAGGAGGTTTT
  GTGAGGGCAAGAACCATTTTGCGGCAGGTGGATTTTTTGGCCAAGGCATTTCACTTCCTGGAACCCCAGC
  TATGGATAAAACGTCCATGGTTAATATGAGTCATGCCAACCCGGGCCTTGCTGACTATTTTGGCGCCAAT
  AGGCATCCTGCTGGTCTTACCTTTCCAACAGCTCCTGGATTTTCTTTTAGCTTCCCTGGTCTGTTTCCTT
  CCGGCTTGTACCACAGGCCTCCTTTGATACCTGCTAGTTCTCCTGTTAAAGGACTATCAAGTACTGAACA
  GACAAACAAAAGTCAAAGTCCCCTCATGACACATCCTCAGATACTGCCAGCTACACAGGATATTTTGAAG
  GCACTATCTAAACACCCATCTGTAGGGGACAATAAGCCAGTGGAGCTCCAGCCCGAGAGGTCCTCTGAAG
  AGAGGCCCTTTGAGAAAATCAGTGACCAGTCAGAGAGTAGTGACCTTGATGATGTCAGTACACCAAGTGG
  CAGTGACCTGGAAACAACCTCGGGCTCTGATCTGGAAAGTGACATTGAAAGTGATAAAGAGAAATTTAAA
  GAAAATGGTAAAATGTTCAAAGACAAAGTAAGCCCTCTTCAGAATCTGGCTTCAATAAATAATAAGAAAG
  AATACAGCAATCATTCCATTTTCTCACCATCTTTAGAGGAGCAGACTGCGGTGTCAGGAGCTGTGAATGA
  TTCTATAAAGGCTATTGCTTCTATTGCTGAAAAATACTTTGGTTCAACAGGACTGGTGGGGCTGCAAGAC
  AAAAAAGTTGGAGCTTTACCTTACCCTTCCATGTTTCCCCTCCCATTTTTTCCAGCATTCTCTCAATCAA
  TGTACCCATTTCCTGATAGAGACTTGAGATCGTTACCTTTGAAAATGGAACCCCAATCACCAGGTGAAGT
  AAAGAAACTGCAGAAGGGCAGCTCTGAGTCCCCCTTTGATCTCACCACTAAGCGAAAGGATGAGAAGCCC
  TTGACTCCAGTCCCCTCCAAGCCTCCAGTGACACCTGCCACAAGCCAAGACCAGCCCCTGGATCTAAGTA
  TGGGCAGTAGGAGTAGAGCCAGTGGGACAAAGCTGACTGAGCCTCGAAAAAACCACGTGTTTGGGGGAAA
  AAAAGGAAGCAACGTCGAATCAAGACCTGCTTCAGATGGTTCCTTGCAGCATGCAAGACCCACTCCTTTC
  TTTATGGACCCTATTTACAGAGTAGAGAAAAGAAAACTAACTGACCCACTTGAAGCTTTAAAAGAGAAAT
  ACTTGAGGCCTTCTCCAGGATTCTTGTTTCACCCACAATTCCAACTGCCTGATCAGAGAACTTGGATGTC
  AGCTATTGAAAACATGGCAGAAAAGCTAGAGAGCTTCAGTGCCCTGAAACCTGAGGCCAGTGAGCTCTTA
  CAGTCAGTGCCCTCTATGTTCAACTTCAGGGCGCCTCCCAATGCCCTGCCAGAGAACCTTCTGCGGAAGG
  GAAAGGAGCGCTATACCTGCAGATACTGTGGCAAGATTTTTCCAAGGTCTGCAAACCTAACACGGCACTT
  GAGAACCCACACAGGAGAGCAGCCTTACAGATGCAAATACTGTGACAGATCATTTAGCATATCTTCTAAC
  TTGCAAAGGCATGTTCGCAACATCCACAATAAAGAGAAGCCATTTAAGTGTCACTTATGTGATAGGTGTT
  TTGGTCAACAAACCAATTTAGACAGACACCTAAAGAAACATGAGAATGGGAACATGTCCGGTACAGCAAC
  ATCGTCGCCTCATTCTGAACTGGAAAGTACAGGTGCGATTCTGGATGACAAAGAAGATGCTTACTTCACA
  GAAATTCGAAATTTCATTGGGAACAGCAACCATGGCAGCCAATCTCCCAGGAATGTGGAGGAGAGAATGA
  ATGGCAGTCATTTTAAAGATGAAAAGGCTTTGGTGACCAGTCAAAATTCAGACTTGCTGGATGATGAAGA
  AGTTGAAGATGAGGTGTTGTTAGATGAGGAGGATGAAGACAATGATATTACTGGAAAAACAGGAAAGGAA
  CCAGTGACAAGTAATTTACATGAAGGAAACCCTGAGGATGACTATGAAGAAACCAGTGCCCTGGAGATGA
  GTTGCAAGACATCCCCAGTGAGGTATAAAGAGGAAGAATATAAAAGTGGACTTTCTGCTCTAGATCATAT
  AAGGCACTTCACAGATAGCCTCAAAATGAGGAAAATGGAAGATAATCAATATTCTGAAGCTGAGCTGTCT
  TCTTTTAGTACTTCCCATGTGCCAGAGGAACTTAAGCAGCCGTTACACAGAAAGTCCAAATCGCAGGCAT
  ATGCTATGATGCTGTCACTGTCTGACAAGGAGTCCCTCCATTCTACATCCCACAGTTCTTCCAACGTGTG
  GCACAGTATGGCCAGGGCTGCGGCGGAATCCAGTGCTATCCAGTCCATAAGCCACGTATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001105077
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105077.3, NP_001098547.3
• RefSeq Size: 4900 bp
• RefSeq ORF: 3351 bp
• Locus ID: 2122
• Cytogenetics: 3q26.2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chronic myeloid leukemia, MAPK signaling pathway, Pathways in cancer
• Gene Summary: 'The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]'
  Transcript Variant: This variant (1) encodes isoform a.