FHL2 (NM_201557) Human Untagged Clone

CAT#: SC317400

FHL2 (untagged)-Human four and a half LIM domains 2 (FHL2), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FHL2
• Synonyms: AAG11; DRAL; FHL-2; SLIM-3; SLIM3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_201557, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACTGAGCGCTTTGACTGCCACCATTGCAACGAATCTCTCTTTGGCAAGAAGTACATCCTGCGGGAGG
  AGAGCCCCTACTGCGTGGTGTGCTTTGAGACCCTGTTCGCCAACACCTGCGAGGAGTGTGGGAAGCCCAT
  CGGCTGTGACTGCAAGGACTTGTCTTACAAGGACCGGCACTGGCATGAAGCCTGTTTCCACTGCTCGCAG
  TGCAGAAACTCACTGGTGGACAAGCCCTTTGCTGCCAAGGAGGACCAGCTGCTCTGTACAGACTGCTATT
  CCAACGAGTACTCATCCAAGTGCCAGGAATGCAAGAAGACCATCATGCCAGGTACCCGCAAGATGGAGTA
  CAAGGGCAGCAGCTGGCATGAGACCTGCTTCATCTGCCACCGCTGCCAGCAGCCAATTGGAACCAAGAGT
  TTCATCCCCAAAGACAATCAGAATTTCTGTGTGCCCTGCTATGAGAAACAACATGCCATGCAGTGCGTTC
  AGTGCAAAAAGCCCATCACCACGGGAGGGGTCACTTACCGGGAGCAGCCCTGGCACAAGGAGTGCTTCGT
  GTGCACCGCCTGCAGGAAGCAGCTGTCTGGGCAGCGCTTCACAGCTCGCGATGACTTTGCCTACTGCCTG
  AACTGCTTCTGTGACTTGTATGCCAAGAAGTGTGCTGGGTGCACCAACCCCATCAGCGGACTTGGTGGCA
  CAAAATACATCTCCTTTGAGGAACGGCAGTGGCATAACGACTGCTTTAACTGTAAGAAGTGCTCCCTCTC
  ACTGGTGGGGCGTGGCTTCCTCACAGAGAGGGACGACATCCTGTGCCCCGACTGTGGGAAAGACATCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_201557
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201557.3, NP_963851.2
• RefSeq Size: 1727 bp
• RefSeq ORF: 840 bp
• Locus ID: 2274
• Cytogenetics: 2q12.2
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 2. Variants 1, 2, 4, 5, 6, 7, and 8 all encode the same isoform (a).