CD1E (NM_030893) Human Untagged Clone

CAT#: SC317622

CD1E (untagged)-Human CD1e molecule (CD1E), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD1E
• Synonyms: CD1A; R2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_030893, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGCTCCTGTTCCTCCTCTTCGAGGGTCTCTGCTGTCCTGGGGAAAATACAGCAGCTCCCCAGGCTC
  TACAATCCTATCATCTAGCAGCAGAGGAGCAGCTGTCCTTCCGCATGCTCCAAACTTCCTCCTTTGCCAA
  CCACAGCTGGGCACACAGTGAGGGCTCAGGATGGCTGGGTGACCTGCAGACTCATGGCTGGGACACTGTC
  TTGGGCACCATCCGCTTTCTGAAGCCCTGGTCCCATGGAAACTTCAGCAAGCAGGAGCTGAAAAACTTAC
  AGTCACTGTTCCAGTTATACTTCCATAGTTTTATCCAGATAGTGCAAGCTTCTGCTGGTCAATTTCAGCT
  TGAATACCCCTTCGAGATCCAGATATTAGCTGGCTGTAGAATGAATGCCCCACAAATCTTCTTAAATATG
  GCATATCAAGGGTCAGATTTCCTGAGTTTCCAAGGAATTTCCTGGGAGCCATCTCCAGGAGCAGGGATCC
  GGGCCCAGAACATCTGTAAAGTGCTCAATCGCTACCTAGATATTAAGGAAATACTGCAAAGCCTTCTTGG
  TCACACCTGCCCTCGATTTCTAGCGGGGCTCATGGAAGCAGGGGAGTCAGAACTGAAACGGAAAGTGAAG
  CCAGAGGCCTGGCTGTCCTGTGGCCCCAGTCCTGGCCCTGGCCGTCTGCAGCTTGTGTGCCATGTCTCAG
  GATTCTACCCAAAGCCCGTGTGGGTGATGTGGATGCGGGGTGAGCAGGAGCAGCGGGGCACTCAGCGAGG
  GGACGTCCTGCCTAATGCTGACGAGACATGGTATCTCCGAGCAACCCTGGATGTGGCGGCTGGGGAGGCA
  GCTGGCCTGTCCTGTCGGGTGAAACACAGCAGTCTAGGGGGCCATGATCTAATCATCCATTGGGGTGGAT
  ATTCCATCTTTCTCATCCTGATCTGTTTGACTGTGATAGTTACCCTGGTCATATTGGTTGTAGTTGACTC
  ACGGTTAAAAAAACAGAGTTCAAATAAGAACATTCTTTCTCCCCACACACCCAGCCCTGTCTTTCTCATG
  GGAGCCAACACTCAGGACACCAAGAATTCAAGACATCAGTTCTGCTTGGCACAAGTATCGTGGATCAAAA
  ACAGAGTATTGAAGAAGTGGAAGACACGCCTAAACCAACTCTGGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_030893
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030893.3, NP_112155.2
• RefSeq Size: 2117 bp
• RefSeq ORF: 1167 bp
• Locus ID: 913
• Cytogenetics: 1q23.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage
• Gene Summary: 'This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members. Many alternatively spliced transcript variants encoding different isoforms have been described. Additional transcript variants have been found; however, their biological validity has not been determined. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform a, also known as 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.