ICA1 (NM_004968) Human Untagged Clone

CAT#: SC317806

ICA1 (untagged)-Human islet cell autoantigen 1, 69kDa (ICA1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ICA1
• Synonyms: ICA69; ICAp69
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004968, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCAGGACACAAATGCAGTTATCCCTGGGACTTACAGGATCGATATGCTCAAGATAAGTCAGTTGTAA
  ATAAGATGCAACAGAAATATTGGGAGACGAAGCAGGCCTTTATTAAAGCCACAGGGAAGAAGGAAGATGA
  ACATGTTGTTGCCTCTGACGCGGACCTGGATGCCAAGCTAGAGCTGTTTCATTCAATTCAGAGAACCTGT
  CTGGACTTATCGAAAGCAATTGTACTCTATCAAAAGAGGATATGTTTCTTGTCTCAAGAAGAAAACGAAC
  TGGGAAAATTTCTTCGATCCCAAGGTTTCCAAGATAAAACCAGAGCAGGAAAGATGATGCAAGCGACAGG
  AAAGGCCCTCTGCTTTTCTTCCCAGCAAAGGTTGGCCTTACGAAATCCTTTGTGTCGATTTCACCAAGAA
  GTGGAGACTTTTCGGCATCGGGCCATCTCAGATACTTGGCTGACGGTGAACCGCATGGAACAGTGCAGGA
  CGGAATATAGAGGAGCACTATTATGGATGAAGGACGTGTCTCAGGAGCTTGATCCAGACCTCTACAAGCA
  AATGGAGAAGTTCAGGAAGGTACAAACACAAGTGCGCCTTGCAAAAAAAAACTTTGACAAATTGAAGATG
  GATGTTTGTCAAAAAGTGGATCTTCTTGGAGCGAGCAGATGCAATCTCTTGTCTCACATGCTAGCAACAT
  ACCAGACCACTCTGCTTCATTTTTGGGAGAAAACTTCTCACACTATGGCAGCCATCCATGAGAGTTTCAA
  AGGTTATCAACCATATGAATTTACTACTTTAAAGAGCTTACAAGACCCTATGAAAAAATTAGTTGAGAAA
  GAAGAGAAGAAGAAAATCAACCAGCAGGAAAGTACAGATGCAGCCGTGCAGGAGCCGAGCCAATTAATTT
  CATTAGAGGAAGAAAACCAGCGCAAGGAATCCTCTAGTTTTAAGACTGAAGATGGAAAAAGTATTTTATC
  TGCCTTAGACAAAGGCTCTACACATACTGCATGCTCAGGACCCATAGATGAACTATTAGACATGAAATCT
  GAGGAAGGTGCTTGCCTGGGACCAGTGGCAGGGACCCCGGAACCTGAAGGTGCTGACAAAGATGACCTGC
  TGCTGTTGAGTGAGATCTTCAATGCTTCCTCCTTGGAAGAGGGCGAGTTCAGCAAAGAGTGGGCCGCTGT
  GTTTGGAGACGGCCAAGTGAAGGAGCCAGTGCCCACTATGGCCCTGGGAGAGCCAGACCCCAAGGCCCAG
  ACAGGCTCAGGTTTCCTTCCTTCGCAGCTTTTAGACCAAAATATGAAAGACTTACAGGCCTCGCTACAAG
  AACCTGCTAAGGCTGCCTCAGACCTGACTGCCTGGTTCAGCCTCTTCGCTGACCTCGACCCACTCTCAAA
  TCCTGATGCTGTTGGGAAAACCGATAAAGAACACGAATTGCTCAATGCATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004968
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004968.3, NP_004959.2
• RefSeq Size: 2458 bp
• RefSeq ORF: 1452 bp
• Locus ID: 3382
• Cytogenetics: 7p21.3
• Domains: ICA69
• Protein Pathways: Type I diabetes mellitus
• Gene Summary: 'This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]'
  Transcript Variant: This variant (2) has an alternate first exon (5' UTR) compared to variant 3. Variants 1, 2, 3, 5, and 6 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.