MGAT3 (NM_002409) Human Untagged Clone

CAT#: SC317905

MGAT3 (untagged)-Human mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (MGAT3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MGAT3
• Synonyms: GNT-III; GNT3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002409 edited
  GTTGCTGAGACCCAGCGGGCGATGGGATGAAGATGAGACGCTACAAGCTCTTTCTCATGT
  TCTGTATGGCCGGCCTGTGCCTCATCTCCTTCCTGCACTTCTTCAAGACCCTGTCCTATG
  TCACCTTCCCCCGAGAACTGGCCTCCCTCAGCCCTAACCTGGTGTCCAGCTTTTTCTGGA
  ACAATGCCCCGGTCACGCCCCAGGCCAGCCCCGAGCCAGGAGGCCCTGACCTGCTGCGTA
  CCCCACTCTACTCCCACTCGCCCCTGCTGCAGCCGCTGCCGCCCAGCAAGGCGGCCGAGG
  AGCTCCACCGGGTGGACTTGGTGCTGCCCGAGGACACCACCGAGTATTTCGTGCGCACCA
  AGGCCGGCGGCGTCTGCTTCAAACCCGGCACCAAGATGCTGGAGAGGCCGCCCCCGGGAC
  GGCCGGAGGAGAAGCCTGAGGGGGCCAACGGCTCCTCGGCCCGGCGGCCACCCCGGTACC
  TCCTGAGCGCCCGGGAGCGCACGGGGGGCCGAGGCGCCCGGCGCAAGTGGGTGGAGTGCG
  TGTGCCTGCCCGGCTGGCACGGACCCAGCTGCGGCGTGCCCACTGTGGTGCAGTACTCCA
  ACCTGCCCACCAAGGAGCGGCTGGTGCCCAGGGAGGTGCCGCGCCGCGTCATCAACGCCA
  TCAACGTCAACCACGAGTTCGACCTGCTGGACGTGCGCTTCCACGAGCTGGGCGACGTGG
  TGGACGCCTTTGTGGTGTGCGAGTCCAACTTCACGGCTTATGGGGAGCCGCGGCCGCTCA
  AGTTCCGGGAGATGCTGACCAATGGCACCTTCGAGTACATCCGCCACAAGGTGCTCTATG
  TCTTCCTGGACCACTTCCCGCCCGGCGGCCGGCAGGACGGCTGGATCGCCGACGACTACC
  TGCGCACCTTCCTCACCCAGGACGGCGTCTCGCGGCTGCGCAACCTGCGGCCCGACGACG
  TCTTCATCATTGACGATGCGGACGAGATCCCGGCCCGTGACGGCGTCCTTTTCCTCAAGC
  TCTACGATGGCTGGACCGAGCCCTTCGCCTTCCACATGCGCAAGTCGCTCTACGGCTTCT
  TCTGGAAGCAGCCGGGCACCCTGGAGGTGGTGTCAGGCTGCACGGTGGACATGCTGCAGG
  CAGTGTATGGGCTGGACGGCATCCGCCTGCGCCGCCGCCAGTACTACACCATGCCCAACT
  TCAGACAGTATGAGAACCGCACCGGCCACATCCTGGTGCAGTGGTCGCTGGGCAGCCCCC
  TGCACTTCGCCGGCTGGCACTGCTCCTGGTGCTTCACGCCCGAGGGCATCTACTTCAAGC
  TCGTGTCCGCCCAGAATGGCGACTTCCCACGCTGGGGTGACTACGAGGACAAGCGGGACC
  TGAACTACATCCGCGGCCTGATCCGCACCGGGGGCTGGTTCGACGGCACGCAGCAGGAGT
  ACCCGCCTGCAGACCCCAGCGAGCACATGTATGCGCCCAAGTACCTGCTGAAGAACTACG
  ACCGGTTCCACTACCTGCTGGACAACCCCTACCAGGAGCCCAGGAGCACGGCGGCGGGCG
  GGTGGCGCCACAGGGGTCCCGAGGGAAGGCCGCCCGCCCGGGGCAAACTGGACGAGGCGG
  AAGTCTAGAGCTGCATGATCTGATAGGGTTTGTGACAGGGCGGGGGTGGCGGCGGCCCCT
  AGCGCTATCTCCCTGCCTCCTGCCGGCTCCTTGGTTCTTGAGGGGACCAGGAGTGGGTGG
  GGAGTGGGGGTGGGGGTAGGGTTTCCCTACTGAAGCCCTTGTGAATCAAGGGTCAGGCCT
  TTGAGCTCAGAAAATATCCCTCCTGTTGGGAGAGGGCGCAGGCCGTGACGTCTGGGTGGC
  CCTTATGACTGCCAAGACTGCTGTGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_002409
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_002409.4.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002409.4, NP_002400.3
• RefSeq Size: 5102 bp
• RefSeq ORF: 1602 bp
• Locus ID: 4248
• Cytogenetics: 22q13.1
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript, and is transcribed from the H20 promoter. Both variants 1 and 2 encode the same protein.