PIF1 (NM_025049) Human Untagged Clone

CAT#: SC318097

PIF1 (untagged)-Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIF1
• Synonyms: C15orf20; PIF
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_025049 edited
  TGAGTGTCGGCACAAACCAGCAGAGGCGGTGACGATGCTCTCGGGCATAGAGGCGGCGGC
  AGGGGAATATGAGGACTCGGAGCTGCGGTGCCGCGTGGCTGTGGAGGAGCTGAGCCCGGG
  CGGGCAGCCGCGAAGGCGCCAGGCCCTGCGCACCGCGGAGCTGAGCCTGGGTCGCAACGA
  GCGCCGCGAGTTGATGCTGCGGCTGCAAGCGCCAGGGCCCGCGGGGCGGCCGCGCTGCTT
  CCCTCTGCGCGCCGCGCGCCTCTTCACGCGTTTCGCCGAGGCCGGGCGCAGCACCCTGCG
  GCTCCCCGCCCACGACACCCCCGGGGCCGGCGCAGTGCAGCTGCTGCTCTCGGACTGCCC
  CCCAGACCGCCTGCGCCGCTTCCTGCGCACATTGCGCCTCAAGCTGGCTGCGGCCCCGGG
  TCCCGGGCCGGCCTCCGCCCGAGCGCAGCTGCTGGGCCCGCGGCCCCGCGACTTCGTCAC
  CATCAGCCCTGTGCAGCCCGAGGAGCGGCGGCTCAGGGCGGCCACCCGGGTTCCGGACAC
  TACGCTGGTGAAGCGGCCTGTGGAGCCCCAGGCTGGGGCCGAGCCTAGCACAGAAGCCCC
  AAGGTGGCCCCTGCCTGTGAAGAGGCTGAGCTTGCCCTCCACCAAGCCACAGCTTTCTGA
  GGAACAGGCTGCTGTGCTGAGGGCCGTCCTGAAAGGCCAGAGCATCTTCTTCACTGGGAG
  TGCAGGAACAGGGAAGTCATATCTGCTAAAGCGAATCCTGGGCTCACTGCCCCCCACAGG
  CACTGTGGCCACTGCCAGCACTGGGGTGGCAGCCTGCCACATCGGGGGCACCACCCTCCA
  TGCCTTTGCAGGCATCGGCTCAGGCCAGGCTCCTCTAGCCCAGTGTGTGGCCCTGGCCCA
  AAGGCCAGGCGTGCGGCAGGGCTGGCTGAACTGCCAGCGGTTGGTCATTGACGAGATCTC
  AATGGTGGAGGCAGACCTGTTTGACAAACTGGAGGCCGTGGCCAGAGCTGTCCGGCAGCA
  GAACAAGCCATTCGGAGGGATCCAGCTCATCATCTGTGGGGACTTTCTGCAGCTGCCACC
  TGTGACCAAGGGCTCCCAGCCCCCACGGTTCTGCTTCCAGTCCAAGAGCTGGAAGAGGTG
  TGTGCCAGTGACCCTGGAGCTGACCAAGGTGTGGAGGCAGGCAGACCAGACCTTCATCTC
  TCTACTGCAGGCCGTGAGGCTAGGCAGGTGTTCAGATGAGGTGACCCGCCAGCTCCAGGC
  CACAGCTTCCCACAAGGTGGGGCGAGATGGGATTGTGGCCACGAGGCTCTGCACCCACCA
  GGATGATGTGGCCCTCACCAACGAGAGGCGGCTTCAGGAGCTGCCAGGTAAGGTACACAG
  ATTTGAGGCTATGGACAGCAACCCTGAGCTGGCCAGTACCCTGGATGCCCAGTGTCCTGT
  TAGCCAGCTCCTTCAACTAAAGCTGGGGGCCCAGGTGATGCTGGTGAAAAACTTATCGGT
  GTCTCGGGGCCTGGTGAATGGTGCCCGAGGGGTGGTAGTTGGGTTCGAGGCAGAAGGGAG
  AGGGCTACCCCAGGTGCGGTTCCTGTGTGGAGTCACTGAGGTCATCCACGCTGACCGCTG
  GACGGTGCAGGCCACCGGGGGCCAGCTCCTCAGTCGGCAGCAGCTGCCCCTCCAGCTGGC
  CTGGGCGATGTCCATCCACAAGAGCCAAGGCATGACCCTGGATTGTGTGGAGATTTCTCT
  GGGCCGTGTGTTTGCCAGTGGCCAGGCCTATGTGGCCCTTTCTCGGGCCCGCAGCCTGCA
  GGGCCTACGTGTGCTGGACTTTGACCCCATGGCGGTTCGCTGTGACCCCCGTGTGCTGCA
  CTTCTATGCCACCCTGCGGCGGGGCAGGAGCCTCAGTCTGGAGTCCCCAGATGATGATGA
  GGCAGCCTCAGACCAGGAGAACATGGACCCAATCCTCTGAGCCTCACCCACAAAGAGAAG
  ACAAAGGGTGGCCTGTGGCCTCCCCGTCCTCCTGCTCCCTAGTGGCCCAGGGCCTCAGGG
  AATAACTGGAGTAGGCAGGCAGTGTCCCCTTCTGTATTTTTTAGGGACTCTCAGCCTCCT
  GGCAGGGTTAAAGGGAGAGTACTTTAAACCCATATACCAGCTGTGCTTCAGTTTCTCTCA
  CTTTGCCCTGGGTAAGCTGCTGTAGGGTCAGAAGTAACCCTTTCTGTGCCAGTTGAGAAT
  GAGCCTGTGTGGTAGCTGATGTCAGAGGACAAAGCTCTCTGCAAGGGCTGGACACAGAGC
  TGCAGAGTCCTGAACATCCCTCCTTTCAGGCTGCAGAAGGGAGAGGCAATGAAGACAGGT
  GCTCCGGAAGCAGCATCAGGGCTCTTGGAGGGGACTGGTGGGGACTCAGGCTGGGTGCAG
  CCTCCAAACAGAGAACGGAACTTAGGTGTGTCTCTACAGCTAGGCCCAGCCTAGCCCAGC
  CCAGAACAAACACCCTTCAGAGCCTAACCAAAGAACATAAGCTGCAAAATGTGCACCCAT
  ATTTTAAGCTGCTTTTTCAGGGGATAAATAGTGTTTGGGGACATTGAAATGGATGTTCTC
  AGGTTGTATTTATTTCGGACAAATAAACTAGAGAATTGTGTAAAAAAAAAAAAAAAAAAA
  AAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025049 unedited
  CCTCATTCCCCCGCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT
  AAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC
  CGCGAATTCGGCACGAGGTGAGTGTCGGCACAAACCAGCAGAGGCGGTGACGATGCTCTC
  GGGCATAGAGGCGGCGGCAGGGGAATATGAGGACTCGGAGCTGCGGTGCCGCGTGGCTGT
  GGAGGAGCTGAGCCCGGGCGGGCAGCCGCGAAGGCGCCAGGCCCTGCGCACCGCGGAGCT
  GAGCCTGGGTCGCAACGAGCGCCGCGAGTTGATGCTGCGGCTGCAAGCGCCAGGGCCCGC
  GGGGCGGCCGCGCTGCTTCCCTCTGCGCGCCGCGCGCCTCTTCACGCGTTTCGCCGAGGC
  CGGGCGCAGCACCCTGCGGCTCCCCGCCCACGACACCCCCGGGGCCGGCGCAGTGCAGCT
  GCTGCTCTCGGACTGCCCCCCAGACCGCCTGCGCCGCTTCCTGCGCACATTGCGCCTCAA
  GCTGGCTGCGGCCCCGGGTCCCGGGCCGGCCTCCGCCCGAGCGCAGCTGCTGGGCCCGCG
  GCCCCGCGACTTCGTCACCATCAGCCCTGTGCAGCCCGAGGAGCGGCGGCTCAGGGCGGC
  CACCCGGGTTCCGGACACTACGCTGGTGAAGCGGCCTGTGGAGCCCCACGCTGGGGCCGC
  GCCTAGCACAGAAGCCCTAGGTGGCCCCTGCCTGTGAAGAGGCTGCACTTGCCCTCCACC
  AGCCCAGTTTCTGAGCACAGGCTGTGGTCTGGGTCCTCCTGACAGGCCATACATTTCTTC
  ATGGTCTGACGAACCGGGAATCCTATTTGTTCAAGCGATCCTGGTCTCCTGTCCCCCCGG
  CCTG
  
• Restriction Sites: Please inquire
• ACCN: NM_025049
• ORF Size: 1926 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_025049.2, NP_079325.2
• RefSeq Size: 2659
• RefSeq ORF: 1926
• Locus ID: 80119
• Gene Summary: This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (a, also known as alpha or alpha-1) initiates translation from the upstream AUG. This isoform localizes to the nucleus (PMID: 17827721). Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.