PCCA (NM_000282) Human Untagged Clone

CAT#: SC318212

PCCA (untagged)-Human propionyl CoA carboxylase, alpha polypeptide (PCCA), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCCA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000282, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGGGTTCTGGGTCGGGACAGCACCGCTGGTCGCTGCCGGACGGCGTGGGCGGTGGCCGCCGCAGC
  AGCTGATGCTGAGCGCGGCGCTGCGGACCCTGAAGCATGTTCTGTACTATTCAAGACAGTGCTTAATGGT
  GTCCCGTAATCTTGGTTCAGTGGGATATGATCCTAATGAAAAAACTTTTGATAAAATTCTTGTTGCTAAT
  AGAGGAGAAATTGCATGTCGGGTTATTAGAACTTGCAAGAAGATGGGCATTAAGACAGTTGCCATCCACA
  GTGATGTTGATGCTAGTTCTGTTCATGTGAAAATGGCGGATGAGGCTGTCTGTGTTGGCCCAGCTCCCAC
  CAGTAAAAGCTACCTCAACATGGATGCCATCATGGAAGCCATTAAGAAAACCAGGGCCCAAGCTGTACAT
  CCAGGTTATGGATTCCTTTCAGAAAACAAAGAATTTGCCAGATGTTTGGCAGCAGAAGATGTCGTTTTCA
  TTGGACCTGACACACATGCTATTCAAGCCATGGGCGACAAGATTGAAAGCAAATTATTAGCTAAGAAAGC
  AGAGGTTAATACAATCCCTGGCTTTGATGGAGTAGTCAAGGATGCAGAAGAAGCTGTCAGAATTGCAAGG
  GAAATTGGCTACCCTGTCATGATCAAGGCCTCAGCAGGTGGTGGTGGGAAAGGCATGCGCATTGCTTGGG
  ATGATGAAGAGACCAGGGATGGTTTTAGATTGTCATCTCAAGAAGCTGCTTCTAGTTTTGGCGATGATAG
  ACTACTAATAGAAAAATTTATTGATAATCCTCGTCATATAGAAATCCAGGTTCTAGGTGATAAACATGGG
  AATGCTTTATGGCTTAATGAAAGAGAGTGCTCAATTCAGAGAAGAAATCAGAAGGTGGTGGAGGAAGCAC
  CAAGCATTTTTTTGGATGCGGAGACTCGAAGAGCGATGGGAGAACAAGCTGTAGCTCTTGCCAGAGCAGT
  AAAATATTCCTCTGCTGGGACCGTGGAGTTCCTTGTGGACTCTAAGAAGAATTTTTATTTCTTGGAAATG
  AATACAAGACTCCAGGTTGAGCATCCTGTCACAGAATGCATTACTGGCCTGGACCTAGTCCAGGAAATGA
  TCCGTGTTGCTAAGGGCTACCCTCTCAGGCACAAACAAGCTGATATTCGCATCAACGGCTGGGCAGTTGA
  ATGTCGGGTTTATGCTGAGGACCCCTACAAGTCTTTTGGTTTACCATCTATTGGGAGATTGTCTCAGTAC
  CAAGAACCGTTACATCTACCTGGTGTCCGAGTGGACAGTGGCATCCAACCAGGAAGTGATATTAGCATTT
  ATTATGATCCTATGATTTCAAAACTAATCACATATGGCTCTGATAGAACTGAGGCACTGAAGAGAATGGC
  AGATGCACTGGATAACTATGTTATTCGAGGTGTTACACATAATATTGCATTACTTCGAGAGGTGATAATC
  AACTCACGCTTTGTAAAAGGAGACATCAGCACTAAATTTCTCTCCGATGTGTATCCTGATGGCTTCAAAG
  GACACATGCTAACCAAGAGTGAGAAGAACCAGTTATTGGCAATAGCATCATCATTGTTTGTGGCATTCCA
  GTTAAGAGCACAACATTTTCAAGAAAATTCAAGAATGCCTGTTATTAAACCAGACATAGCCAACTGGGAG
  CTCTCAGTAAAATTGCATGATAAAGTTCATACCGTAGTAGCATCAAACAATGGGTCAGTGTTCTCGGTGG
  AAGTTGATGGGTCGAAACTAAATGTGACCAGCACGTGGAACCTGGCTTCGCCCTTATTGTCTGTCAGCGT
  TGATGGCACTCAGAGGACTGTCCAGTGTCTTTCTCGAGAAGCAGGTGGAAACATGAGCATTCAGTTTCTT
  GGTACAGTGTACAAGGTGAATATCTTAACCAGACTTGCCGCAGAATTGAACAAATTTATGCTGGAAAAAG
  TGACTGAGGACACAAGCAGTGTTCTGCGTTCCCCGATGCCCGGAGTGGTGGTGGCCGTCTCTGTCAAGCC
  TGGAGACGCGGTAGCAGAAGGTCAAGAAATTTGTGTGATTGAAGCCATGAAAATGCAGAATAGTATGACA
  GCTGGGAAAACTGGCACGGTGAAATCTGTGCACTGTCAAGCTGGAGACACAGTTGGAGAAGGGGATCTGC
  TCGTGGAGCTGGAATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000282
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000282.3, NP_000273.2
• RefSeq Size: 2577 bp
• RefSeq ORF: 2187 bp
• Locus ID: 5095
• Cytogenetics: 13q32.3
• Domains: biotin_lipoyl, CPSase_L_D2, CPSase_L_chain, Biotin_carb_C
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: 'The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]'
  Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.