Smoothelin (SMTN) (NM_006932) Human Untagged Clone

CAT#: SC318389

SMTN (untagged)-Human smoothelin (SMTN), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMTN
• Synonyms: FLJ35168; FLJ35365; FLJ35620; FLJ38597
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006932, the custom clone sequence may differ by one or more nucleotides
  ATGGCGGACGAGGCCTTAGCTGGGCTGGATGAGGGAGCCCTTCGGAAGCTGCTGGAGGTC
  ACAGCAGATCTGGCAGAGCGGCGGCGCATCCGCTCAGCCATCCGGGAACTGCAGCGGCAG
  GAGCTGGAGCGCGAGGAGGAGGCCCTGGCATCCAAGCGTTTCCGTGCCGAGCGGCAGGAC
  AACAAGGAGAACTGGCTGCACTCTCAGCAGCGGGAAGCTGAGCAGCGGGCTGCCCTGGCA
  CGGCTGGCAGGGCAGCTGGAGTCCATGAACGATGTGGAGGAATTGACTGCACTGTTGCGA
  AGCGCTGGTGAGTATGAGGAGCGCAAGCTGATCCGAGCTGCCATCCGCCGTGTACGGGCT
  CAGGAGATTGAGGCTGCCACCTTGGCTGGGAGGTTGTACAGCGGGCGTCCCAACAGTGGC
  TCAAGAGAGGACAGCAAGGGGCTAGCGGCACACAGGCTGGAACAGTGTGAGGTGCCAGAG
  CGAGAGGAACAGGAACAGCAGGCAGAGGTTTCAAAGCCAACCCCCACCCCTGAAGGCACC
  AGCCAGGATGTGACCACAGTGACACTCCTGCTGCGAGCCCCACCTGGGAGCACATCCAGC
  TCACCTGCCTCACCCAGCAGTTCACCCACCCCTGCCTCTCCTGAGCCTCCATTGGAGCCT
  GCCGAGGCCCAGTGCCTTACAGCTGAGGTTCCAGGCAGCCCAGAGCCACCCCCCAGCCCA
  CCCAAGACCACCAGCCCTGAGCCTCAGGAGTCTCCAACGCTCCCCAGCACTGAGGGCCAG
  GTGGTCAACAAGCTTCTGTCTGGCCCCAAAGAGACCCCTGCTGCCCAGAGCCCCACCAGA
  GGCCCCTCTGACACCAAGAGAGCAGACGTGGCTGGACCCCGACCCTGCCAACGCTCCCTG
  TCGGTGCTCAGCCCCCGCCAACCAGCCCAGAACCGAGAGTCCACCCCCCTTGCCAGCGGA
  CCTTCCTCATTCCAGCGGGCTGGCTCTGTGCGGGATCGTGTCCACAAGTTCACATCTGAT
  TCTCCTATGGCTGCTAGGCTCCAGGATGGCACACCCCAGGCTGCCCTAAGTCCCCTGACC
  CCCGCAAGGCTCCTGGGCCCCTCCCTCACCAGCACCACCCCTGCCTCCTCCTCCAGCGGC
  TCCTCCTCTCGGGGCCCCAGTGATACCTCCTCCCGGTTCAGCAAGGAGCAACGAGGAGTA
  GCCCAGCCCCTGGCCCAGCTTCGAAGCTGCCCCCAGGAGGAGGGCCCCAGGGGGCGGGGC
  TTGGCTGCTAGGCCCCTTGAAAACAGAGCAGGGGGGCCTGTGGCACGTTCAGAGGAGCCT
  GGTGCCCCGCTGCCCGTGGCCGTCGGCACTGCCGAGCCAGGGGGCAGTATGAAGACCACA
  TTCACCATCGAGATCAAGGACGGCCGTGGCCAGGCCTCCACAGGCCGGGTGCTGCTGCCC
  ACAGGCAACCAGAGGGCAGAACTGACACTGGGGCTGCGGGCGCCCCCGACCCTACTCAGC
  ACCAGTAGTGGGGGCAAGAGCACCATCACCCGTGTCAACAGCCCTGGGACCCTGGCTCGG
  CTGGGCAGTGTCACTCATGTCACCAGCTTCAGCCATGCCCCCCCCAGTAGCCGAGGAGGC
  TGCAGCATCAAGATGGAAGCAGAGCCAGCAGAGCCTCTCGCTGCAGCAGTGGAAGCGGCC
  AATGGGGCTGAGCAGACCCGAGTGAACAAAGCACCAGAAGGGCGGAGCCCTCTGAGCGCT
  GAGGAGCTGATGACTATTGAGGATGAAGGAGTCTTGGACAAGATGCTGGATCAGAGCACG
  GACTTTGAAGAGCGGAAGCTCATCCGGGCTGCACTTCGTGAGCTCCGACAAAGGAAGAGA
  GACCAGCGGGACAAGGAGCGGGAACGGCGGCTGCAGGAGGCACGGGGCCGGCCAGGGGAG
  GGGCGCGGCAACACAGCCACTGAGACCACCACGAGGCACAGCCAGCGGGCAGCTGATGGC
  TCTGCTGTCAGCACTGTTACCAAGACTGAGCGGCTCGTCCACTCCAATGATGGCACACGG
  ACGGCCCGCACCACCACAGTGGAGTCGAGTTTCGTGAGGCGCTCGGAGAATGGCAGTGGC
  AGCACCATGATGCAAACCAAGACCTTCTCCTCTTCCTCCTCATCCAAGAAGATGGGCAGC
  ATCTTCGACCGCGAGGACCAGGCCAGCCCACGGGCCGGCAGCCTGGCGGCGCTCGAGAAA
  CGGCAGGCCGAGAAGAAGAAAGAGCTGATGAAGGCGCAGAGTCTGCCCAAGACCTCAGCC
  TCCCAGGCGCGCAAGGCCATGATTGAGAAGCTGGAGAAGGAGGGCGCGGCCGGCAGCCCT
  GGCGGACCCCGCGCAGCCGTGCAGCGATCCACCAGCTTCGGGGTCCCCAACGCCAACAGC
  ATCAAGCAGATGCTGCTGGACTGGTGTCGAGCCAAGACTCGCGGCTACGAGCACGTCGAC
  ATCCAGAACTTCTCCTCCAGCTGGAGTGATGGGATGGCCTTCTGTGCCCTGGTGCACAAC
  TTCTTCCCTGAGGCCTTCGACTATGGGCAGCTTAGCCCTCAGAACCGACGCCAGAACTTC
  GAGGTGGCCTTCTCATCTGCGGAGATGCTGGTGGACTGTGTGCCCCTGGTGGAGGTGGAC
  GACATGATGATCATGGGCAAGAAGCCTGACCCCAAGTGTGTCTTCACCTATGTGCAGTCG
  CTCTACAACCACCTGCGACGCCACGAACTGCGCCTGCGCGGCAAGAATGTC
  
• Restriction Sites: Please inquire
• ACCN: NM_006932
• Insert Size: 3175 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006932.3, NP_008863.3
• RefSeq Size: 3175 bp
• RefSeq ORF: 2754 bp
• Locus ID: 6525
• Cytogenetics: 22q12.2
• Domains: CH
• Gene Summary: 'This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2011]'
  Transcript Variant: This variant (3) has an alternate 5' exon, which results in a downstream AUG start codon, and lacks an in-frame exon in the middle region, compared to variant 4. The resulting isoform (c, also referred to as L1, B and c1) is shorter at the N-terminus and lacks an internal segment, compared to isoform d. This isoform is highly expressed in all vascularized organs and represents a vascular smooth muscle-specific form. This transcript also includes an alternate downstream translation initiation site, which results in the smoothelin-A product, a visceral smooth muscle-specific form.