Phospholipase C beta 2 (PLCB2) (NM_004573) Human Untagged Clone

CAT#: SC318538

PLCB2 (untagged)-Human phospholipase C, beta 2 (PLCB2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLCB2
• Synonyms: PLC-beta-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004573, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTCTGCTCAACCCTGTCCTGCTGCCCCCCAAGGTGAAGGCCTATCTGAGCCAAGGGGAGCGCTTCA
  TCAAATGGGATGATGAAACTACAGTTGCCTCTCCAGTTATCCTCCGTGTGGATCCTAAGGGCTACTACTT
  ATACTGGACGTATCAAAGTAAGGAGATGGAGTTTCTGGATATCACCAGCATCCGGGATACTCGCTTTGGG
  AAGTTTGCCAAGATGCCCAAGAGCCAGAAGCTCCGGGACGTCTTCAACATGGACTTTCCTGATAACAGTT
  TCCTGCTGAAGACACTCACGGTGGTGTCCGGCCCGGACATGGTGGACCTCACCTTCCACAACTTCGTCTC
  CTACAAGGAGAACGTGGGCAAGGCCTGGGCTGAGGACGTACTGGCCCTAGTCAAACATCCGCTGACGGCC
  AACGCCTCCCGCAGCACCTTCCTGGACAAGATCCTTGTGAAGCTCAAGATGCAGCTCAACTCTGAAGGGA
  AGATTCCGGTGAAGAACTTTTTCCAGATGTTTCCTGCTGACCGCAAGCGGGTGGAAGCTGCTCTCAGTGC
  CTGCCACCTCCCCAAAGGCAAAAATGACGCCATCAATCCTGAGGACTTCCCAGAACCTGTCTACAAGAGT
  TTCCTCATGAGCCTCTGTCCTCGGCCAGAAATAGATGAGATCTTCACTTCTTACCATGCTAAGGCCAAAC
  CCTACATGACGAAGGAGCACCTGACCAAATTCATCAACCAGAAACAGCGGGACTCCCGGCTTAACTCCCT
  GCTGTTCCCGCCAGCACGGCCTGACCAGGTGCAGGGCCTCATCGACAAGTATGAGCCCAGTGGCATCAAT
  GCACAGAGGGGCCAGCTGTCACCTGAAGGCATGGTCTGGTTTCTCTGTGGGCCAGAGAACAGCGTGCTGG
  CCCAGGACAAGCTGCTGCTCCACCACGACATGACGCAGCCACTCAATCATTACTTCATCAACTCGTCCCA
  CAACACCTACCTGACAGCCGGCCAGTTCTCAGGCCTCTCCTCGGCTGAGATGTACCGCCAGGTGCTGCTC
  TCTGGCTGCCGTTGCGTGGAGCTAGACTGCTGGAAGGGGAAACCCCCTGACGAGGAGCCCATTATCACCC
  ATGGCTTCACCATGACCACAGACATCTTCTTCAAAGAAGCAATTGAGGCTATTGCAGAAAGCGCCTTTAA
  GACCTCCCCCTATCCCATCATCCTGTCGTTTGAGAACCATGTGGACTCACCCCGCCAGCAGGCTAAGATG
  GCTGAGTATTGCCGGACGATCTTTGGGGATATGCTGCTCACAGAGCCCCTGGAAAAGTTCCCACTAAAAC
  CAGGTGTCCCCCTGCCCAGCCCTGAGGATCTCAGGGGCAAGATCCTCATCAAGAACAAGAAGAACCAGTT
  TTCTGGCCCCACCTCCTCCAGTAAGGATACTGGTGGGGAGGCTGAGGGCAGCAGCCCACCCAGTGCCCCT
  GCAGGTGAGGGCACAGTGTGGGCTGGCGAGGAAGGGACTGAGCTGGAGGAGGAGGAGGTGGAAGAGGAAG
  AGGAGGAGGAGTCAGGAAACCTGGATGAAGAAGAGATTAAGAAGATGCAGTCGGATGAGGGCACAGCGGG
  CCTGGAAGTGACGGCTTATGAGGAGATGTCCAGCCTAGTCAATTACATCCAGCCCACCAAGTTCGTCTCC
  TTTGAGTTCTCTGCCCAAAAGAACCGAAGTTATGTCATCTCGTCCTTCACAGAGCTCAAGGCATATGACC
  TGCTCTCCAAGGCCTCGGTGCAGTTTGTGGACTACAACAAGCGCCAGATGAGCCGCATTTACCCCAAGGG
  AACCCGCATGGACTCCTCCAACTACATGCCCCAGATGTTCTGGAATGCTGGATGCCAGATGGTTGCCCTC
  AACTTCCAGACGATGGACTTGCCCATGCAGCAGAACATGGCAGTATTTGAGTTCAACGGGCAGAGCGGCT
  ACCTCCTCAAGCATGAGTTCATGCGCCGGCCGGACAAGCAGTTCAACCCCTTCTCAGTGGACCGCATCGA
  CGTGGTGGTGGCCACCACCCTTTCCATTACGGTGATCTCTGGGCAGTTCCTGTCAGAACGCAGCGTGCGC
  ACCTATGTAGAAGTGGAGCTGTTTGGCCTTCCTGGGGACCCCAAGAGGCGCTATCGAACTAAGCTGTCAC
  CCAGTACTAACTCCATCAATCCTGTCTGGAAGGAGGAGCCCTTTGTCTTTGAGAAGATCTTGATGCCTGA
  GCTGGCCTCCCTCAGAGTGGCTGTGATGGAGGAAGGCAACAAGTTTCTTGGACACCGCATCATCCCCATC
  AATGCCCTAAATTCTGGGTACCACCACCTGTGCCTGCACAGTGAGAGCAACATGCCCCTCACCATGCCTG
  CGCTCTTCATCTTCCTGGAGATGAAGGACTACATACCTGGTGCTTGGGCAGATCTCACTGTGGCCCTCGC
  CAACCCCATTAAGTTCTTCAGTGCCCATGACACGAAGTCTGTGAAGCTCAAGGAGGCCATGGGAGGTCTG
  CCTGAGAAGCCCTTCCCACTGGCGAGTCCAGTTGCCAGCCAGGTCAATGGGGCGTTGGCCCCAACGAGCA
  ATGGGTCACCAGCAGCCAGGGCCGGGGCCAGGGAAGAGGCTATGAAAGAAGCTGCGGAGCCGCGGACCGC
  CAGCCTGGAGGAGCTCCGGGAGCTAAAGGGCGTGGTGAAGCTGCAGCGGCGGCACGAGAAGGAGCTGCGA
  GAGTTGGAGCGGCGCGGAGCGCGGCGCTGGGAGGAGCTGCTGCAGCGGGGCGCGGCGCAGCTGGCGGAGC
  TCGGGCCACCGGGCGTGGGGGGCGTCGGGGCCTGCAAGCTCGGTCCCGGCAAGGGCTCTCGCAAGAAGAG
  GAGCCTGCCCCGCGAGGAGAGCGCCGGAGCCGCGCCGGGCGAGGGCCCTGAGGGCGTGGACGGGCGCGTG
  CGGGAGCTGAAAGACAGGCTGGAGCTGGAGCTGCTGCGGCAGGGCGAGGAGCAGTACGAGTGCGTTCTGA
  AGCGCAAGGAGCAGCACGTGGCCGAGCAAATCTCCAAAATGATGGAGCTGGCCAGAGAGAAACAGGCGGC
  AGAGCTGAAGGCCCTGAAGGAGACGTCGGAGAACGACACCAAAGAGATGAAGAAAAAGCTGGAGACAAAG
  AGACTGGAGCGGATCCAGGGCATGACCAAAGTCACCACAGACAAGATGGCCCAGGAGAGGTTGAAGAGAG
  AGATTAACAACTCCCACATCCAGGAAGTAGTGCAGGTGATCAAGCAGATGACGGAGAACTTGGAGAGGCA
  CCAGGAGAAGCTGGAGGAGAAGCAGGCGGCTTGCCTGGAACAGATACGGGAGATGGAAAAGCAGTTCCAG
  AAGGAGGCGCTGGCAGAGTACGAGGCCAGGATGAAGGGTCTGGAGGCAGAGGTGAAGGAGTCGGTGAGGG
  CCTGCCTCAGGACCTGCTTTCCCTCCGAGGCCAAGGACAAGCCTGAGAGGGCCTGCGAGTGCCCCCCAGA
  GCTGTGTGAGCAGGACCCACTCATAGCAAAGGCAGATGCCCAGGAGAGCCGCCTCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004573
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004573.2, NP_004564.2
• RefSeq Size: 4694 bp
• RefSeq ORF: 3558 bp
• Locus ID: 5330
• Cytogenetics: 15q15.1
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Chemokine signaling pathway, Gap junction, GnRH signaling pathway, Huntington's disease, Inositol phosphate metabolism, Long-term depression, Long-term potentiation, Melanogenesis, Metabolic pathways, Phosphatidylinositol signaling system, Taste transduction, Vascular smooth muscle contraction, Wnt signaling pathway
• Gene Summary: 'The protein encoded by this gene is a phosphodiesterase that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol. The encoded protein is activated by G proteins and has been shown to be involved in the type 2 taste receptor signal transduction pathway. In addition, nuclear factor kappa B can regulate the transcription of this gene, whose protein product is also an important regulator of platelet responses. [provided by RefSeq, Jan 2017]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).