PLCG 2 (PLCG2) (NM_002661) Human Untagged Clone

CAT#: SC318575

PLCG2 (untagged)-Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLCG2
• Synonyms: APLAID; FCAS3; PLC-gamma-2; PLC-IV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002661, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCACCACGGTCAATGTAGATTCCCTTGCGGAATATGAGAAGAGCCAGATCAAGAGAGCCCTGGAGC
  TGGGGACGGTGATGACTGTGTTCAGCTTCCGCAAGTCCACCCCCGAGCGGAGAACCGTCCAGGTGATCAT
  GGAGACGCGGCAGGTGGCCTGGAGCAAGACCGCTGACAAGATCGAGGGCTTCTTGGATATCATGGAAATA
  AAAGAAATCCGCCCAGGGAAGAACTCCAAAGATTTCGAGCGAGCAAAAGCAGTTCGCCAGAAAGAAGACT
  GCTGCTTCACCATCCTATATGGCACTCAGTTCGTCCTCAGCACGCTCAGCTTGGCAGCTGACTCTAAAGA
  GGATGCAGTTAACTGGCTCTCTGGCTTGAAAATCTTACACCAGGAAGCGATGAATGCGTCCACGCCCACC
  ATTATCGAGAGTTGGCTGAGAAAGCAGATATATTCTGTGGATCAAACCAGAAGAAACAGCATCAGTCTCC
  GAGAGTTGAAGACCATCTTGCCCCTGATCAACTTTAAAGTGAGCAGTGCCAAGTTCCTTAAAGATAAGTT
  TGTGGAAATAGGAGCACACAAAGATGAGCTCAGCTTTGAACAGTTCCATCTCTTCTATAAAAAACTTATG
  TTTGAACAGCAAAAATCGATTCTCGATGAATTCAAAAAGGATTCGTCCGTGTTCATCCTGGGGAACACTG
  ACAGGCCGGATGCCTCTGCTGTTTACCTGCATGACTTCCAGAGGTTTCTCATACATGAACAGCAGGAGCA
  TTGGGCTCAGGATCTGAACAAAGTCCGTGAGCGGATGACAAAGTTCATTGATGACACCATGCGTGAAACT
  GCTGAGCCTTTCTTGTTTGTGGATGAGTTCCTCACGTACCTGTTTTCACGAGAAAACAGCATCTGGGATG
  AGAAGTATGACGCGGTGGACATGCAGGACATGAACAACCCCCTGTCTCATTACTGGATCTCCTCGTCACA
  TAACACGTACCTTACAGGTGACCAGCTGCGGAGCGAGTCGTCCCCAGAAGCTTACATCCGCTGCCTGCGC
  ATGGGCTGTCGCTGCATTGAACTGGACTGCTGGGACGGGCCCGATGGGAAGCCGGTCATCTACCATGGCT
  GGACGCGGACTACCAAGATCAAGTTTGACGACGTCGTGCAGGCCATCAAAGACCACGCCTTTGTTACCTC
  GAGCTTCCCAGTGATCCTGTCCATCGAGGAGCACTGCAGCGTGGAGCAACAGCGTCACATGGCCAAGGCC
  TTCAAGGAAGTATTTGGCGACCTGCTGTTGACGAAGCCCACGGAGGCCAGTGCTGACCAGCTGCCCTCGC
  CCAGCCAGCTGCGGGAGAAGATCATCATCAAGCATAAGAAGCTGGGCCCCCGAGGCGATGTGGATGTCAA
  CATGGAGGACAAGAAGGACGAACACAAGCAACAGGGGGAGCTGTACATGTGGGATTCCATTGACCAGAAA
  TGGACTCGGCACTACTGCGCCATTGCCGATGCCAAGCTGTCCTTCAGTGATGACATTGAACAGACTATGG
  AGGAGGAAGTGCCCCAGGATATACCCCCTACAGAACTACATTTTGGGGAGAAATGGTTCCACAAGAAGGT
  GGAGAAGAGGACGAGTGCCGAGAAGTTGCTGCAGGAATACTGCATGGAGACGGGGGGCAAGGATGGCACC
  TTCCTGGTTCGGGAGAGCGAGACCTTCCCCAATGACTACACCCTGTCCTTCTGGCGGTCAGGCCGGGTCC
  AGCACTGCCGGATCCGCTCCACCATGGAGGGCGGGACCCTGAAATACTACTTGACTGACAACCTCACCTT
  CAGCAGCATCTATGCCCTCATCCAGCACTACCGCGAGACGCACCTGCGCTGCGCCGAGTTCGAGCTGCGG
  CTCACGGACCCTGTGCCCAACCCCAACCCCCACGAGTCCAAGCCGTGGTACTATGACAGCCTGAGCCGCG
  GAGAGGCAGAGGACATGCTGATGAGGATTCCCCGGGACGGGGCCTTCCTGATCCGGAAGCGAGAGGGGAG
  CGACTCCTATGCCATCACCTTCAGGGCTAGGGGCAAGGTAAAGCATTGTCGCATCAACCGGGACGGCCGG
  CACTTTGTGCTGGGGACCTCCGCCTATTTTGAGAGTCTGGTGGAGCTCGTCAGTTACTACGAGAAGCATT
  CACTCTACCGAAAGATGAGACTGCGCTACCCCGTGACCCCCGAGCTCCTGGAGCGCTACAATATGGAAAG
  AGATATAAACTCCCTCTACGACGTCAGCAGAATGTATGTGGATCCCAGTGAAATCAATCCGTCCATGCCT
  CAGAGAACCGTGAAAGCTCTGTATGACTACAAAGCCAAGCGAAGCGATGAGCTGAGCTTCTGCCGTGGTG
  CCCTCATCCACAATGTCTCCAAGGAGCCCGGGGGCTGGTGGAAAGGAGACTATGGAACCAGGATCCAGCA
  GTACTTCCCATCCAACTACGTCGAGGACATCTCAACTGCAGACTTCGAGGAGCTAGAAAAGCAGATTATT
  GAAGACAATCCCTTAGGGTCTCTTTGCAGAGGAATATTGGACCTCAATACCTATAACGTCGTGAAAGCCC
  CTCAGGGAAAAAACCAGAAGTCCTTTGTCTTCATCCTGGAGCCCAAGCAGCAGGGCGATCCTCCGGTGGA
  GTTTGCCACAGACAGGGTGGAGGAGCTCTTTGAGTGGTTTCAGAGCATCCGAGAGATCACCTGGAAGATT
  GACACCAAGGAGAACAACATGAAGTACTGGGAGAAGAACCAGTCCATCGCCATCGAGCTCTCTGACCTGG
  TTGTCTACTGCAAACCAACCAGCAAAACCAAGGACAACTTAGAAAATCCTGACTTCCGAGAAATCCGCTC
  CTTTGTGGAGACGAAGGCTGACAGCATCATCAGACAGAAGCCCGTCGACCTCCTGAAGTACAATCAAAAG
  GGCCTGACCCGCGTCTACCCAAAGGGACAAAGAGTTGACTCTTCAAACTACGACCCCTTCCGCCTCTGGC
  TGTGCGGTTCTCAGATGGTGGCACTCAATTTCCAGACGGCAGATAAGTACATGCAGATGAATCACGCATT
  GTTTTCTCTCAATGGGCGCACGGGCTACGTTCTGCAGCCTGAGAGCATGAGGACAGAGAAATATGACCCG
  ATGCCACCCGAGTCCCAGAGGAAGATCCTGATGACGCTGACAGTCAAGGTTCTCGGTGCTCGCCATCTCC
  CCAAACTTGGACGAAGTATTGCCTGTCCCTTTGTAGAAGTGGAGATCTGTGGAGCCGAGTATGACAACAA
  CAAGTTCAAGACGACGGTTGTGAATGATAATGGCCTCAGCCCTATCTGGGCTCCAACACAGGAGAAGGTG
  ACATTTGAAATTTATGACCCAAACCTGGCATTTCTGCGCTTTGTGGTTTATGAAGAAGATATGTTCAGCG
  ATCCCAACTTTCTTGCTCATGCCACTTACCCCATTAAAGCAGTCAAATCAGGATTCAGGTCCGTTCCTCT
  GAAGAATGGGTACAGCGAGGACATAGAGCTGGCTTCCCTCCTGGTTTTCTGTGAGATGCGGCCAGTCCTG
  GAGAGCGAAGAGGAACTTTACTCCTCCTGTCGCCAGCTGAGGAGGCGGCAAGAAGAACTGAACAACCAGC
  TCTTTCTGTATGACACACACCAGAACTTGCGCAATGCCAACCGGGATGCCCTGGTTAAAGAGTTCAGTGT
  TAATGAGAACCAGCTCCAGCTGTACCAGGAGAAATGCAACAAGAGGTTAAGAGAGAAGAGAGTCAGCAAC
  AGCAAGTTTTACTCATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002661
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002661.4, NP_002652.2
• RefSeq Size: 8707 bp
• RefSeq ORF: 3798 bp
• Locus ID: 5336
• Cytogenetics: 16q23.3
• Domains: C2, PI-PLC-X, SH2, SH3, PI-PLC-Y, PH
• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway, Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, VEGF signaling pathway, Vibrio cholerae infection
• Gene Summary: 'The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014]'