COL5A2 (NM_000393) Human Untagged Clone

CAT#: SC318638

COL5A2 (untagged)-Human collagen, type V, alpha 2 (COL5A2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL5A2
• Synonyms: EDSC; EDSCL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000393, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGGCAAACTGGGCGGAAGCAAGACCTCTCCTCATTCTTATTGTTTTATTAGGGCAATTTGTCTCAA
  TAAAAGCCCAGGAAGAAGACGAGGATGAAGGATATGGTGAAGAAATAGCCTGCACTCAGAATGGCCAGAT
  GTACTTAAACAGGGACATTTGGAAACCTGCCCCTTGTCAGATCTGTGTCTGTGACAATGGAGCCATTCTC
  TGTGACAAGATAGAATGCCAGGATGTGCTGGACTGTGCCGACCCTGTAACGCCCCCTGGGGAATGCTGTC
  CTGTCTGTTCACAAACACCTGGAGGTGGCAATACAAATTTTGGTAGAGGAAGAAAGGGACAAAAGGGAGA
  ACCAGGATTAGTGCCTGTTGTAACAGGCATACGTGGTCGTCCAGGACCGGCAGGACCTCCAGGATCACAG
  GGACCAAGAGGAGAGCGAGGGCCAAAAGGAAGACCTGGCCCTCGTGGACCTCAGGGAATTGATGGAGAAC
  CAGGTGTTCCTGGTCAACCTGGTGCTCCAGGACCTCCTGGACATCCGTCCCACCCAGGACCCGATGGCTT
  GAGCAGGCCGTTTTCAGCTCAAATGGCTGGGTTGGATGAAAAATCTGGACTTGGGAGTCAAGTAGGACTA
  ATGCCTGGCTCTGTGGGTCCTGTTGGCCCAAGGGGACCACAGGGTTTACAAGGACAGCAAGGTGGTGCAG
  GACCTACAGGACCTCCTGGTGAACCTGGTGATCCTGGACCAATGGGTCCGATTGGTTCACGTGGACCAGA
  GGGCCCTCCTGGTAAACCTGGGGAAGATGGTGAACCTGGCAGAAATGGAAATCCTGGTGAAGTGGGATTT
  GCAGGATCTCCGGGAGCTCGTGGATTTCCTGGGGCTCCTGGTCTTCCAGGTCTGAAGGGTCACCGAGGAC
  ACAAAGGTCTTGAAGGCCCTAAAGGTGAAGTTGGAGCACCTGGTTCCAAGGGTGAAGCTGGCCCCACTGG
  TCCAATGGGTGCCATGGGTCCTCTGGGTCCGAGGGGAATGCCAGGAGAGAGAGGGAGACTTGGGCCACAG
  GGTGCTCCTGGACAACGAGGTGCACATGGTATGCCTGGAAAACCTGGACCAATGGGTCCTCTTGGGATAC
  CAGGCTCTTCTGGTTTTCCAGGAAATCCTGGAATGAAGGGAGAAGCAGGTCCTACAGGGGCGCGAGGCCC
  TGAAGGTCCTCAGGGGCAGAGAGGTGAAACTGGGCCCCCAGGTCCAGTTGGCTCTCCAGGTCTTCCTGGT
  GCAATAGGAACTGATGGTACTCCTGGTGCCAAAGGCCCAACGGGCTCTCCGGGTACCTCTGGTCCTCCTG
  GCTCAGCAGGGCCTCCTGGATCTCCAGGACCTCAGGGTAGCACTGGTCCTCAGGGAATTCGAGGCCAACC
  GGGTGATCCAGGAGTTCCAGGTTTCAAAGGAGAAGCTGGCCCAAAAGGGGAACCAGGGCCACATGGTATT
  CAGGGTCCGATAGGCCCACCCGGTGAAGAAGGCAAAAGAGGTCCCAGAGGTGACCCAGGAACAGTTGGTC
  CTCCAGGGCCAGTGGGAGAAAGGGGTGCTCCTGGCAATCGTGGTTTTCCAGGCTCTGATGGTTTACCTGG
  GCCAAAGGGTGCTCAAGGAGAACGGGGTCCTGTAGGTTCTTCAGGACCCAAAGGAAGCCAGGGGGATCCA
  GGACGTCCAGGGGAACCTGGGCTTCCAGGTGCTCGGGGTTTGACAGGAAATCCTGGTGTTCAAGGTCCTG
  AAGGAAAACTTGGACCTTTGGGTGCGCCAGGGGAAGATGGCCGTCCAGGTCCTCCAGGCTCCATAGGAAT
  CAGAGGGCAGCCCGGGAGCATGGGCCTTCCAGGCCCCAAAGGTAGCAGTGGTGACCCTGGGAAACCTGGA
  GAAGCAGGAAATGCTGGAGTTCCTGGGCAGAGGGGAGCTCCTGGAAAAGATGGTGAAGTTGGTCCTTCTG
  GTCCTGTGGGCCCGCCGGGTCTAGCTGGTGAAAGAGGAGAACAAGGACCTCCAGGCCCCACAGGTTTTCA
  GGGGCTTCCTGGTCCTCCAGGGCCTCCTGGAGAAGGTGGAAAACCAGGTGATCAAGGTGTTCCTGGAGAT
  CCCGGAGCAGTTGGCCCGTTAGGACCTAGAGGAGAACGAGGAAATCCTGGGGAAAGAGGAGAACCTGGGA
  TAACTGGACTCCCTGGTGAGAAGGGAATGGCTGGAGGACATGGTCCTGATGGCCCAAAAGGCAGTCCAGG
  TCCATCTGGGACCCCTGGAGATACAGGCCCACCAGGTCTTCAAGGTATGCCGGGAGAAAGAGGAATTGCA
  GGAACTCCTGGCCCCAAGGGTGACAGAGGTGGCATAGGAGAAAAAGGTGCTGAAGGCACAGCTGGAAATG
  ATGGTGCAAGAGGTCTTCCAGGTCCTTTGGGCCCTCCAGGTCCGGCAGGTCCTACTGGAGAAAAGGGTGA
  ACCTGGTCCTCGAGGTTTAGTTGGCCCTCCTGGCTCCCGGGGCAATCCTGGTTCTCGAGGTGAAAATGGG
  CCAACTGGAGCTGTTGGTTTTGCCGGACCCCAGGGTCCTGACGGACAGCCTGGAGTAAAAGGTGAACCTG
  GAGAGCCAGGACAGAAGGGAGATGCTGGTTCTCCTGGACCACAAGGTTTAGCAGGATCCCCTGGCCCTCA
  TGGTCCTAATGGTGTTCCTGGACTAAAAGGTGGTCGAGGAACCCAAGGTCCGCCTGGTGCTACAGGATTT
  CCTGGTTCTGCGGGCAGAGTTGGACCTCCAGGCCCTGCTGGAGCTCCAGGACCTGCGGGACCCCTAGGGG
  AACCCGGGAAGGAGGGACCTCCAGGTCTTCGTGGGGACCCTGGCTCTCATGGGCGTGTGGGAGATCGAGG
  ACCAGCTGGCCCCCCTGGTGGCCCAGGAGACAAAGGGGACCCAGGAGAAGATGGGCAACCTGGTCCAGAT
  GGCCCCCCTGGTCCAGCTGGAACGACCGGGCAGAGAGGAATTGTTGGCATGCCTGGGCAACGTGGAGAGA
  GAGGCATGCCCGGCCTACCAGGCCCAGCGGGAACACCAGGAAAAGTAGGACCAACTGGTGCAACAGGAGA
  TAAAGGTCCACCTGGACCTGTGGGGCCCCCAGGCTCCAATGGTCCTGTAGGGGAACCTGGACCAGAAGGT
  CCAGCTGGCAATGATGGTACCCCAGGACGGGATGGTGCTGTTGGAGAACGTGGTGATCGTGGAGACCCTG
  GGCCTGCAGGTCTGCCAGGCTCTCAGGGTGCCCCTGGAACTCCTGGCCCTGTGGGTGCTCCAGGAGATGC
  AGGACAAAGAGGAGATCCGGGTTCTCGGGGTCCTATAGGACCACCTGGTCGAGCTGGGAAACGTGGATTA
  CCTGGACCCCAAGGACCTCGTGGTGACAAAGGTGATCATGGAGACCGAGGCGACAGAGGTCAGAAGGGCC
  ACAGAGGCTTTACTGGTCTTCAGGGTCTTCCTGGCCCTCCTGGTCCAAATGGTGAACAAGGAAGTGCTGG
  AATCCCTGGACCATTTGGCCCAAGAGGTCCTCCAGGCCCAGTTGGTCCTTCAGGTAAAGAAGGAAACCCT
  GGGCCACTTGGGCCAATTGGACCTCCAGGTGTACGAGGCAGTGTAGGAGAAGCAGGACCTGAGGGCCCTC
  CTGGTGAGCCTGGCCCACCTGGCCCTCCGGGTCCCCCTGGCCACCTTACAGCTGCTCTTGGGGATATCAT
  GGGGCACTATGATGAAAGCATGCCAGATCCACTTCCTGAGTTTACTGAAGATCAGGCGGCTCCTGATGAC
  AAAAACAAAACGGACCCAGGGGTTCATGCTACCCTGAAGTCACTCAGTAGTCAGATTGAAACCATGCGCA
  GCCCCGATGGCTCGAAAAAGCACCCAGCCCGCACGTGTGATGACCTAAAGCTTTGCCATTCCGCAAAGCA
  GAGTGGTGAATACTGGATTGATCCTAACCAAGGATCTGTTGAAGATGCAATCAAAGTTTACTGCAACATG
  GAAACAGGAGAAACATGTATTTCAGCAAACCCATCCAGTGTACCACGTAAAACCTGGTGGGCCAGTAAAT
  CTCCTGACAATAAACCTGTTTGGTATGGTCTTGATATGAACAGAGGGTCTCAGTTCGCTTATGGAGACCA
  CCAATCACCTAATACAGCCATTACTCAGATGACTTTTTTGCGCCTTTTATCAAAAGAAGCCTCCCAGAAC
  ATCACTTACATCTGTAAAAACAGTGTAGGATACATGGACGATCAAGCTAAGAACCTCAAAAAAGCTGTGG
  TTCTCAAAGGGGCAAATGACTTAGATATCAAAGCAGAGGGAAATATTAGATTCCGGTATATCGTTCTTCA
  AGACACTTGCTCTAAGCGGAATGGAAATGTGGGCAAGACTGTCTTTGAATATAGAACACAGAATGTGGCA
  CGCTTGCCCATCATAGATCTTGCTCCTGTGGATGTTGGCGGCACAGACCAGGAATTCGGCGTTGAAATTG
  GGCCAGTTTGTTTTGTGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000393
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000393.3, NP_000384.2
• RefSeq Size: 6930 bp
• RefSeq ORF: 4500 bp
• Locus ID: 1290
• Cytogenetics: 2q32.2
• Domains: COLFI, VWC, Collagen
• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• Gene Summary: 'This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq, Jul 2008]'