SH3BP2 (NM_001122681) Human Untagged Clone

CAT#: SC318934

SH3BP2 (untagged)-Human SH3-domain binding protein 2 (SH3BP2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SH3BP2
• Synonyms: 3BP-2; 3BP2; CRBM; CRPM; RES4-23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001122681, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCTGAAGAGATGCATTGGCCTGTCCCTATGAAGGCCATTGGTGCCCAGAACCTGCTAACCATGC
  CTGGGGGCGTGGCCAAGGCTGGCTACCTGCACAAGAAGGGCGGTACCCAGCTGCAGCTGCTGAAATGGCC
  CCTGCGCTTTGTCATCATCCACAAACGCTGCGTCTACTACTTCAAGAGTAGCACCTCTGCCTCCCCGCAG
  GGCGCCTTCTCCCTGAGTGGCTATAACCGGGTGATGCGGGCGGCTGAGGAGACCACGTCCAACAACGTTT
  TCCCCTTCAAGATCATCCATATCAGCAAGAAGCACCGCACGTGGTTCTTCTCGGCCTCCTCCGAGGAGGA
  GCGCAAGAGCTGGATGGCCTTGCTGCGCAGGGAGATTGGCCACTTCCACGAAAAGAAAGACCTGCCCTTG
  GACACCAGCGACTCCAGCTCGGACACAGACAGCTTCTACGGCGCAGTTGAGCGGCCTGTGGATATCAGCC
  TTTCCCCGTACCCCACGGACAATGAAGACTATGAGCACGACGATGAGGATGACTCCTACCTGGAGCCTGA
  CTCCCCGGAGCCCGGAAGGCTTGAGGATGCCCTGATGCACCCACCGGCTTACCCACCACCCCCAGTGCCC
  ACGCCCAGGAAGCCAGCCTTCTCTGACATGCCCCGGGCCCACTCCTTTACCTCCAAGGGCCCCGGTCCCC
  TACTGCCACCCCCGCCCCCTAAGCACGGCCTCCCAGATGTTGGCCTGGCTGCTGAGGACTCCAAGAGGGA
  CCCACTGTGCCCGAGGCGGGCTGAGCCTTGCCCCAGGGTACCTGCTACCCCCCGAAGGATGAGCGATCCC
  CCTCTGAGCACCATGCCCACCGCACCCGGCCTCCGGAAACCCCCTTGCTTCCGGGAGAGTGCCAGCCCCA
  GCCCGGAGCCCTGGACCCCTGGCCACGGGGCCTGCTCCACTTCCAGTGCTGCCATCATGGCCACTGCCAC
  CTCCAGAAACTGTGACAAACTCAAGTCCTTCCACCTGTCCCCCCGAGGACCACCCACATCTGAGCCCCCA
  CCTGTGCCAGCCAACAAGCCCAAGTTCCTGAAGATAGCTGAAGAGGACCCCCCAAGGGAGGCAGCCATGC
  CCGGACTCTTTGTGCCCCCCGTGGCTCCCCGGCCTCCTGCGCTGAAGCTGCCAGTGCCTGAGGCCATGGC
  GCGGCCCGCAGTCCTGCCCAGGCCAGAGAAGCCGCAGCTCCCGCACCTCCAGCGATCACCCCCCGATGGG
  CAGAGTTTCAGGAGCTTCTCCTTTGAAAAGCCCCGGCAACCCTCACAGGCTGACACTGGCGGGGACGACT
  CGGACGAGGACTATGAGAAGGTGCCACTGCCCAACTCGGTCTTCGTCAACACCACGGAGTCCTGCGAAGT
  GGAAAGGTTGTTCAAGGCTACAAGCCCCCGGGGAGAGCCCCAGGATGGACTCTACTGCATCCGGAACTCC
  TCTACCAAGTCGGGGAAGGTCCTGGTTGTGTGGGACGAAACCTCTAACAAAGTGAGGAACTATCGCATTT
  TTGAGAAGGACTCTAAGTTCTACCTGGAGGGCGAGGTCCTGTTTGTGAGTGTGGGCAGCATGGTGGAGCA
  CTACCACACCCACGTGCTGCCCAGCCACCAGAGCCTGCTGCTGCGGCACCCCTACGGCTACACTGGGCCT
  AGGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001122681
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001122681.1, NP_001116153.1
• RefSeq Size: 9068 bp
• RefSeq ORF: 1686 bp
• Locus ID: 6452
• Cytogenetics: 4p16.3
• Protein Families: Druggable Genome
• Protein Pathways: Natural killer cell mediated cytotoxicity
• Gene Summary: 'The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (2) represents use of an alternative promoter and 5' UTR and uses a downstream translation start site, compared to variant 3. The resulting isoform (a) has a shorter N-terminus, compared to isoform b. Variants 1 and 2 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.