PUS1 (NM_001002019) Human Untagged Clone

CAT#: SC319107

PUS1 (untagged)-Human pseudouridylate synthase 1 (PUS1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PUS1
• Synonyms: MLASA1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001002019.1 GTCGCGAATGGGGCAGGAGCGAGCCTCTCTGGTCCCGACGCGGGTGGCCCGGGTCTCCTC
  GACTCCTGAGGAAAGCCCACCGGGCGGGGCGGGAGCTCGCCGCGCATGGCCGGGAACGCG
  GAGCCGCCGCCCGCCGGAGCCGCATGCCCCCAGGACCGGAGGTCCTGCAGCGGCCGGGCC
  GGGGGCGACCGCGTCTGGGAGGACGGAGAACATCCGGCGAAGAAGCTCAAGAGCGGTGGC
  GACGAGGAGCGGCGCGAGAAGCCGCCCAAGCGGAAGATCGTGCTGCTCATGGCCTATTCG
  GGCAAGGGCTACCACGGCATGCAGAGGAATGTCGGGTCCTCACAATTCAAAACAATTGAA
  GATGACTTGGTGTCCGCCCTCGTCCGGTCAGGCTGTATTCCTGAAAATCATGGTGAGGAC
  ATGAGGAAAATGTCCTTCCAGCGCTGCGCCCGGACAGACAAGGGTGTGTCCGCAGCCGGC
  CAGGTGGTATCCCTGAAGGTGTGGCTGATTGACGACATTCTAGAAAAGATCAACAGCCAC
  CTTCCCTCTCACATTCGGATTCTGGGACTGAAGCGGGTCACGGGCGGGTTTAACTCCAAG
  AACAGATGTGATGCCAGGACCTATTGCTACCTGCTGCCCACGTTTGCCTTTGCGCACAAG
  GACCGGGACGTTCAGGATGAGACCTACCGCCTGAGCGCCGAGACGCTGCAGCAGGTCAAC
  AGGCTCCTGGCCTGCTACAAGGGCACGCACAACTTCCACAATTTCACCTCGCAGAAGGGG
  CCGCAGGATCCCAGTGCCTGCCGCTACATCCTGGAGATGTACTGCGAGGAACCCTTTGTG
  CGGGAGGGCCTGGAGTTTGCGGTGATCAGGGTGAAGGGCCAGAGCTTCATGATGCATCAG
  ATCCGGAAGATGGTCGGCCTGGTGGTGGCCATTGTGAAGGGTTATGCCCCTGAGAGCGTG
  CTGGAGCGCAGCTGGGGCACAGAGAAGGTGGACGTGCCCAAGGCGCCCGGACTCGGCCTG
  GTCCTGGAGAGGGTGCACTTCGAGAAGTACAACCAGCGCTTTGGCAACGATGGGCTGCAT
  GAGCCGCTGGACTGGGCGCAGGAGGAAGGAAAGGTCGCAGCCTTCAAGGAGGAGCACATC
  TACCCCACCATCATCGGCACCGAGCGGGACGAACGCTCCATGGCCCAGTGGCTGAGCACC
  TTGCCCATCCACAACTTCAGTGCCACCGCTCTCACGGCAGGTGGCACGGGCGCCAAGGTG
  CCCAGTCCCCTGGAAGGCAGTGAAGGGGACGGAGACACTGACTGAGGCGATGGGAGCTGC
  CCACCAGAGTGCCTCTGAGCAGCTCACAGTGTGTGCCCAGATGTGCCACCCCTGTGGGCA
  GCAAGAAGCTGGGATCGCTGCAGCCATGTTTTCCCGGCCATGCCGGCGTTGTAACCTCAG
  GACCTTCCCTTGTAGGAACAGCCTTTCTCGAATCTGTTTTCAGCTCTTGCATTGCATAGA
  TGAACCTCAGCATGTAAAGAACTATTTTTTTAAAGAAGTGATTTTCTTATTAAACAAGTA
  CAAATTTTGCTTAGTCAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001002019.1 unedited
  GTACTATTGTATACGACTCCTATAGGGCGGCCGGGTAATTCGGCACGAGGGTCGCGAATG
  GGGCAGGAGCGAGCCTCTCTGGTCCCGACGCGGGTGGCCCGGGTCTCCTCGACTCCTGAG
  GAAAGCCCACCGGGCGGGGCGGGAGCTCGCCGCGCATGGCCGGGAACGCGGAGCCGCCGC
  CCGCCGGAGCCGCATGCCCCCAGGACCGGAGGTCCTGCAGCGGCCGGGCCGGGGGCGACC
  GCGTCTGGGAGGACGGAGAACATCCGGCGAAGAAGCTCAAGAGCGGTGGCGACGAGGAGC
  GGCGCGAGAAGCCGCCCAAGCGGAAGATCGTGCTGCTCATGGCCTATTCGGGCAAGGGCT
  ACCACGGCATGCAGAGGAATGTCGGGTCCTCACAATTCAAAACAATTGAAGATGACTTGG
  TGTCCGCCCTCGTCCGGTCAGGCTGTATTCCTGAAAATCATGGTGAGGACATGAGGAAAA
  TGTCCTTCCAGCGCTGCGCCCGGACAGACAAGGGTGTGTCCGCAGCCGGCCAGGTGGTAT
  CCCTGAAGGTGTGGCTGATTGACGACATTCTAGAAAAGATCAACAGCCACCTTCCCTCTC
  ACATTCGGATTCTGGGACTGAAGCGGGTCACGGGCGGGTTTAACTCCAAGAACAGATGTG
  ATGCCAGGACCTATTGCTACCTGCTGCCCACGTTTGCCTTTGCGCACAAAGACCGGGACG
  TTCAGATGAGACCTACCGCCTGAGCGCCGAGACGCTGCAGCAGTCAACAGGCTCCTGGCC
  TGCTACAGGGCACGCACAACTTTCCACAATTTCACCTCGCAGAGGGGCCGCAGATCCCAG
  TGCCTGCCGCTACATCTGAGATGTACTGCGAGACCCTTTGTGCGGAGGGCCTGAGTTGCG
  GTGATCAGGGTGAAGGGCCAG
• Restriction Sites: Please inquire
• ACCN: NM_001002019
• ORF Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001002019.1, NP_001002019.1
• RefSeq Size: 1660
• RefSeq ORF: 1200
• Locus ID: 80324
• Gene Summary: This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2).