PPP1R3C (NM_005398) Human Untagged Clone

CAT#: SC319243

PPP1R3C (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3C (PPP1R3C)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP1R3C
• Synonyms: PPP1R5; PTG
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005398.3 GGGGAATTTGTGCAGGCAGCGGGTGCTGGCTTTTAGGGTCCGCCGCCTCTCTGCCTAATG
  AGCTGCACCAGAATGATCCAGGTTTTAGATCCACGTCCTTTGACAAGTTCGGTCATGCCC
  GTGGATGTGGCCATGAGGCTTTGCTTGGCACATTCACCACCTGTGAAGAGTTTCCTGGGC
  CCGTACGATGAATTTCAACGACGACATTTTGTGAATAAATTAAAGCCCCTGAAATCATGT
  CTCAATATAAAACACAAAGCCAAATCACAGAATGACTGGAAGTGCTCACACAACCAAGCC
  AAGAAGCGCGTTGTGTTTGCTGACTCCAAGGGCCTCTCTCTCACTGCGATCCATGTCTTC
  TCCGACCTCCCAGAAGAACCAGCGTGGGATCTGCAGTTTGATCTCTTGGACCTTAATGAT
  ATCTCCTCTGCCTTAAAACACCACGAGGAGAAAAACTTGATTTTAGATTTCCCTCAGCCT
  TCAACCGATTACTTAAGTTTCCGGAGCCACTTTCAGAAGAACTTTGTCTGTCTGGAGAAC
  TGCTCATTGCAAGAGCGAACAGTGACAGGGACTGTTAAAGTCAAAAATGTGAGTTTTGAG
  AAGAAAGTTCAGATCCGTATCACTTTCGATTCTTGGAAAAACTACACTGACGTAGACTGT
  GTCTATATGAAAAATGTGTATGGTGGCACAGATAGTGATACCTTCTCATTTGCCATTGAC
  TTACCCCCTGTCATTCCAACTGAGCAGAAAATTGAGTTCTGCATTTCTTACCATGCTAAT
  GGGCAAGTCTTTTGGGACAACAATGATGGTCAGAATTATAGAATTGTTCATGTTCAATGG
  AAGCCTGATGGGGTGCAGACACAGATGGCACCCCAGGACTGTGCATTCCACCAGACGTCT
  CCTAAGACAGAGTTAGAGTCAACAATCTTTGGCAGTCCGAGGCTGGCTAGTGGGCTCTTC
  CCAGAGTGGCAGAGCTGGGGGAGAATGGAGAACTTGGCCTCTTATCGATGAATTAAGCAA
  CAATGTAACTGGTCTTGACTTGTCATATTCCCCCATGCAATCCTAGGTCTGTATTGCTCA
  ATTTTAGGAAGCCTTTGCTACTCCATCAGTAGGTTTAGATTTGAGCTTTTGAGACCTGGC
  TATGGAAAAGAAAGACACTTGAGAATTTAGTGTTGGGGTCTGTACAGATGATGCTACCCA
  ATTTGGCTTTGAAGGATCAAGTAACAGGTTGAAAACTATTTTTATAAAGGTAATACTTTT
  TCAGTTCCCTTCTTCCTTCCCTCTCAATCCACTAGCTTTCATGTTGGGCAAGGAAAAGTT
  GAGGAAGGATGGCTGATGGTGATGGAAAGCTGTGTTAATGGTATGAGGAATGTGTGAAAA
  GTATACACAAAGGGCTCTGAAGCTCAAGTCAGAGGAGTGGGAGGTCTGATCATTGTTGGT
  GGAAAAACGTAAGGTTATTTTGTGTTTTTAAGTTGGTTTTACAATTCTTTCCTGGGGAAA
  TTATTTCTGGAGGGGAAAAAGATCCATTCTACGTATCCTTGTGGAGAAAAGCTAAATAAC
  CTTTAAGAATGTGGGTGGTATTGGAGAAAGAAGATGAATTATAGCTCCGGAGAATCAAGA
  TCTTAAGTGAAGCCTTTCTGTTCAGATGTGATCTATAAAAAATCATAATTTGGGGAAAGT
  TTAAGCAAATCTGGCTTTGTAGTCCTGATGTTATAAGTGACTTTGTGATCAAACTGTCAG
  GCTTGGGTTCTTGTTATAGAATGCTTGGTATAGAAAAACCATGCCATCATTAATGGCTAA
  CAACACGTAGGGACTTCATGTCATGTCAAAGATAGCTCTTTGCAAGTGCCTTGATTAAAC
  CAGAAAACTGTCATCGTTTAACCCAAATATCTGAATGGTCATCTGGTAACTCATGGGTTT
  TTGGCCTCATAAGATGGTCCACTCTGTACACAGGCATTCCTCCTGCAATAATGTTGTATC
  TTTGAGACCGTTGTCAGTGTACACAACTCACATCCTTCATATTGAAGGTGACTCATTTTT
  CTGCACACTTTTTTGATGTGATGCTTGACGTGAGGCCCGACACTAGGATTCTCAATGCAA
  GAATCCAGTACCTTGCACATAGAAGTAGCAACCCATCCCTTGCCTATTTTCATCTTGCTG
  TTTTCTTTTTTTTAAAAAAATGGATGTGACTTGTTTTGAATGTTTTGTATTATACTTGTT
  TTTGTGTGTGCATAAATTCATTCTGTAGGATCTTAAGAAAAAGAGTCCCAGAATGTTGCT
  TCTATTATTGTGCACAACCATTGAGAGGTGTTACGAGAATGCAGTTAATTTTAACATGTG
  TGATGTGCCATGGTGGAAAAGTACTATCGGAATAACTCTGCAGTGACAGAATTTGAAGTT
  TGGCTAGCATCCATACTTTTCTACTGTAAATATTTCACTCTCCTCTAGCTATCCTTGATG
  AGCTTCTCACTTTAAGAATAAATGTGTTTGATACAAAAAAAAAAAAAAAAGAAAAAAAAA
  AAAA
• Restriction Sites: Please inquire
• ACCN: NM_005398
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005398.3, NP_005389.1
• RefSeq Size: 2647 bp
• RefSeq ORF: 954 bp
• Locus ID: 5507
• Cytogenetics: 10q23.32
• Domains: CBM_21
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway
• Gene Summary: 'This gene encodes a carbohydrate binding protein that is a subunit of the protein phosphatase 1 (PP1) complex. PP1 catalyzes reversible protein phosphorylation, which is important in a wide range of cellular activities. The encoded protein affects glycogen biosynthesis by activating glycogen synthase and limiting glycogen breakdown by reducing glycogen phosphorylase activity. DNA hypermethylation of this gene has been found in colorectal cancer patients. The encoded protein also interacts with the laforin protein, which is a protein tyrosine phosphatase implicated in Lafora disease. [provided by RefSeq, Sep 2016]'