Creatine kinase B type (CKB) (NM_001823) Human Untagged Clone

CAT#: SC319293

CKB (untagged)-Human creatine kinase, brain (CKB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CKB
• Synonyms: B-CK; BCK; CKBB; CPK-B; HEL-211; HEL-S-29
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001823.3 CCTGCGTCGCTCCGGGAGCTGCCGACGGACGGAGCGCCCCCGCCCCCGCCCGGCCGCCCG
  CCCGCCGCCGCCATGCCCTTCTCCAACAGCCACAACGCACTGAAGCTGCGCTTCCCGGCC
  GAGGACGAGTTCCCCGACCTGAGCGCCCACAACAACCACATGGCCAAGGTGCTGACCCCC
  GAGCTGTACGCGGAGCTGCGCGCCAAGAGCACGCCGAGCGGCTTCACGCTGGACGACGTC
  ATCCAGACAGGCGTGGACAACCCGGGCCACCCGTACATCATGACCGTGGGCTGCGTGGCG
  GGCGACGAGGAGTCCTACGAAGTGTTCAAGGATCTCTTCGACCCCATCATCGAGGACCGG
  CACGGCGGCTACAAGCCCAGCGATGAGCACAAGACCGACCTCAACCCCGACAACCTGCAG
  GGCGGCGACGACCTGGACCCCAACTACGTGCTGAGCTCGCGGGTGCGCACGGGCCGCAGC
  ATCCGTGGCTTCTGCCTCCCCCCGCACTGCAGCCGCGGGGAGCGCCGAGCCATCGAGAAG
  CTCGCGGTGGAAGCCCTGTCCAGCCTGGACGGCGACCTGGCGGGCCGATACTACGCGCTC
  AAGAGCATGACGGAGGCGGAGCAGCAGCAGCTCATCGACGACCACTTCCTCTTCGACAAG
  CCCGTGTCGCCCCTGCTGCTGGCCTCGGGCATGGCCCGCGACTGGCCCGACGCCCGCGGT
  ATCTGGCACAATGACAATAAGACCTTCCTGGTGTGGGTCAACGAGGAGGACCACCTGCGG
  GTCATCTCCATGCAGAAGGGGGGCAACATGAAGGAGGTGTTCACCCGCTTCTGCACCGGC
  CTCACCCAGATTGAAACTCTCTTCAAGTCTAAGGACTATGAGTTCATGTGGAACCCTCAC
  CTGGGCTACATCCTCACCTGCCCATCCAACCTGGGCACCGGGCTGCGGGCAGGTGTGCAT
  ATCAAGCTGCCCAACCTGGGCAAGCATGAGAAGTTCTCGGAGGTGCTTAAGCGGCTGCGA
  CTTCAGAAGCGAGGCACAGGCGGTGTGGACACGGCTGCGGTGGGCGGGGTCTTCGACGTC
  TCCAACGCTGACCGCCTGGGCTTCTCAGAGGTGGAGCTGGTGCAGATGGTGGTGGACGGA
  GTGAAGCTGCTCATCGAGATGGAACAGCGGCTGGAGCAGGGCCAGGCCATCGACGACCTC
  ATGCCTGCCCAGAAATGAAGCCCGGCCCACACCCGACACCAGCCCTGCTGCTTCCTAACT
  TATTGCCTGGGCAGTGCCCACCATGCACCCCTGATGTTCGCCGTCTGGCGAGCCCTTAGC
  CTTGCTGTAGAGACTTCCGTCACCCTTGGTAGAGTTTATTTTTTTGATGGCTAAGATACT
  GCTGATGCTGAAATAAACTAGGGTTTTGGCCTGCCTGAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001823
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001823.3, NP_001814.2
• RefSeq Size: 1431 bp
• RefSeq ORF: 1146 bp
• Locus ID: 1152
• Cytogenetics: 14q32.33
• Domains: ATP-gua_Ptrans
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008]'