UROS (NM_000375) Human Untagged Clone

CAT#: SC319379

UROS (untagged)-Human uroporphyrinogen III synthase (UROS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UROS
• Synonyms: UROIIIS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000375.1 CCCACGCGACCCCAGTCTGAGGTGCGGGGTCCTGGGGCCCGGCGCGGGTGGCCGCCGCGG
  CCCCTCGGGCTGCGTGGGGAGGGGGCTTCCGCCCCTGTTGTCATTGCTCCTGCAGCCTTT
  TCGCTGGGACTGCGCGACACCGCCCCCCGACCGGGTGCCCGCTGTGTGCCAGGCCGGGTG
  CTGGGCACGGTCCCGCGAGTGCCCTATAAGGACTGCCAGGCAATAATGAAGGTTCTTTTA
  CTGAAGGATGCGAAGGAAGATGACTGTGGCCAGGATCCGTATATCAGGGAATTAGGATTA
  TATGGACTTGAAGCCACTTTGATCCCTGTTTTATCGTTTGAGTTTTTGTCTCTTCCCAGT
  TTCTCTGAGAAGCTTTCTCATCCTGAAGATTACGGGGGACTCATTTTTACCAGCCCCAGA
  GCAGTGGAAGCAGCAGAGTTATGTTTGGAGCAAAACAATAAAACTGAAGTCTGGGAAAGG
  TCTCTGAAAGAAAAATGGAATGCCAAGTCAGTGTATGTGGTTGGAAATGCTACTGCTTCT
  CTAGTGAGTAAAATTGGCCTGGATACAGAAGGAGAAACCTGTGGAAATGCAGAAAAGCTT
  GCAGAATATATTTGTTCCAGGGAGTCCTCAGCACTGCCTCTTCTATTTCCCTGTGGAAAC
  CTCAAAAGAGAAATCCTGCCAAAAGCGCTCAAGGACAAAGGGATTGCCATGGAAAGCATA
  ACTGTGTATCAGACAGTTGCACACCCAGGAATCCAAGGGAACCTGAACAGCTACTATTCC
  CAGCAGGGGGTTCCAGCCAGCATCACATTTTTTAGTCCCTCTGGCCTCACATACAGTCTC
  AAGCACATTCAGGAGTTATCTGGTGACAATATCGATCAAATTAAGTTTGCAGCCATCGGC
  CCCACTACGGCTCGCGCGCTGGCCGCCCAGGGCCTTCCTGTAAGCTGCACTGCAGAGAGC
  CCCACGCCACAAGCCCTGGCCACTGGCATCAGGAAGGCTCTCCAGCCCCATGGCTGCTGC
  TGAGTCAGCCACCTAGCGCTGGCCCCATGCAGCCTCCCTGGGCTGGGCTGGCTCTGGATG
  GAGCCAGGCATCGGCAAGGGCTCTCGGGAGCTGCTGCCGTCAGACTCCTGCCTCAAGCCT
  GAGTGGAAGCACCTGAGGACCGGGGATCGGGACCTGACCTGGGGCTGGCCTCAGGCCCAC
  GTGCACGTGACTGCCCTCTGTGGAAGCCAGCTTAAACCCTAGCCCTGTGAGAGCTTCCTG
  TGCCCAGCAGGAAGGAAGTCAAATAAACCACACTGACTACCTGTGCTTACTGAAAAAAAA
  AAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000375
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000375.1, NP_000366.1
• RefSeq Size: 1279 bp
• RefSeq ORF: 798 bp
• Locus ID: 7390
• Cytogenetics: 10q26.2
• Domains: HEM4
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Porphyrin and chlorophyll metabolism
• Gene Summary: 'The protein encoded by this gene catalyzes the fourth step of porphyrin biosynthesis in the heme biosynthetic pathway. Defects in this gene cause congenital erythropoietic porphyria (Gunther's disease). [provided by RefSeq, Jul 2008]'