POLR2E (NM_002695) Human Untagged Clone

CAT#: SC319449

POLR2E (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR2E
• Synonyms: hRPB25; hsRPB5; RPABC1; RPB5; XAP4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002695.2 GGCGGCGGCGGCGGAGGCTGCCATGGACGACGAGGAGGAGACGTACCGGCTCTGGAAAAT
  CCGCAAGACCATCATGCAGCTGTGCCACGACCGTGGCTATCTGGTGACCCAGGACGAGCT
  TGACCAGACCCTGGAGGAGTTCAAAGCCCAATTTGGGGACAAGCCGAGTGAGGGGCGGCC
  GCGGCGCACGGACCTCACCGTGCTGGTGGCCCACAACGATGACCCCACCGACCAGATGTT
  TGTGTTCTTTCCAGAGGAGCCCAAGGTGGGCATCAAGACCATCAAGGTGTACTGCCAGCG
  CATGCAGGAGGAGAACATCACACGGGCTCTCATCGTGGTGCAGCAGGGCATGACACCCTC
  CGCCAAGCAGTCCCTGGTCGACATGGCCCCCAAGTACATCCTGGAGCAGTTTCTGCAGCA
  GGAGCTGCTCATCAACATCACGGAGCACGAGCTAGTCCCTGAGCACGTCGTCATGACCAA
  GGAGGAGGTGACAGAGCTGCTGGCCCGATATAAGCTCCGAGAGAACCAGCTGCCCAGGAT
  CCAGGCGGGGGACCCTGTGGCGCGCTACTTTGGGATAAAGCGTGGGCAGGTGGTGAAGAT
  CATCCGGCCCAGTGAGACGGCTGGCAGGTACATCACCTACCGGCTGGTGCAGTAGCTACC
  GCCTGACAGCCCCTAGAGGCGGACACACAGCGACCCCCATCCCTGCAGGACAAACGCCCC
  TGCCCTGCCAGAATCCGGCCCCCACAGCTCTCACGGCTGCTGCTCCTCTGGACTCCCCAA
  GGCAGGTGGCCTCCACCCACGTTCTCCCGTCCTGGGGTGAGGCTTCCTGTGGCCCAGCCC
  GCCCCATTCACCTGTGGATTTGTGCGAGATGCAGCCTCAGAAGGAACAAGGCCCCCAGAG
  GGAGGTCACCTGGGGGCAGCTGGTGCCGGGTCTTCACCCAGACCACGCTGGGTCCCCTCT
  GTTGGGGGTTTGGGGTCCGGGTCTCCCACCAGCCACTGCTTCCTCCTGGGCCCTCGGCCT
  TCCACCCCTCGTCTTCCCTCCCTCGGGGGCCCTGATGCGTGGCGGCCCCCACCCGGCCTC
  GGCTCTTTACTCCATTCACAGCCGTGCACGCGCTCAAGCCACCAGGGTGCGAGATGCCAG
  CTCTGGAGTTCTCGGTTGTTGTAGGAGGTTGGGTGTTTTCAAATGGTAAAGATGTTTTGA
  GCAAATAAATTTGCTTGATACAGAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002695
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002695.2, NP_002686.2
• RefSeq Size: 1238 bp
• RefSeq ORF: 633 bp
• Locus ID: 5434
• Cytogenetics: 19p13.3
• Domains: RNA_pol_Rpb5_C, RNA_pol_Rpb5_N
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: 'This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.