CHRNB1 (NM_000747) Human Untagged Clone

CAT#: SC319546

CHRNB1 (untagged)-Human cholinergic receptor, nicotinic, beta 1 (muscle) (CHRNB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRNB1
• Synonyms: ACHRB; CHRNB; CMS1D; CMS2A; CMS2C; SCCMS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000747.2 AGCGGCTCTCTGAGCGAAGTCACTGAGCGAGCCGCCAGGCTATGACCCCAGGGGCTCTGC
  TGATGCTGCTGGGGGCGCTGGGGGCGCCGCTCGCCCCAGGCGTCCGCGGCTCGGAGGCGG
  AGGGTCGACTCCGGGGGAAACTTTTCTCTGGCTATGATAGCTCCGTGCGGCCAGCGCGGG
  AGGTGGGAGACCGTGTCAGGGTCAGCGTTGGTCTCATCCTGGCGCAACTCATCAGCCTGA
  ACGAGAAGGATGAAGAGATGAGCACAAAGGTGTACTTAGACCTGGAGTGGACTGACTACA
  GGCTGAGCTGGGACCCTGCGGAGCACGACGGCATCGATTCGCTCCGCATCACGGCGGAAT
  CCGTGTGGCTCCCTGACGTGGTGCTACTGAACAACAATGATGGGAATTTTTACGTGGCTC
  TGGACATTAGCGTCGTGGTGTCCTCCGACGGCTCCGTGCGTTGGCAACCCCCGGGCATCT
  ATCGCAGCAGCTGCAGCATCCAGGTCACCTACTTCCCCTTCGACTGGCAGAATTGCACTA
  TGGTGTTCAGCTCCTACAGCTACGACAGCTCGGAGGTCAGCCTGCAGACAGGCCTGGGTC
  CTGACGGGCAAGGGCATCAGGAAATCCACATTCATGAAGGGACTTTCATTGAGAATGGCC
  AGTGGGAGATTATCCACAAGCCCTCTCGGCTAATCCAGCCTCCAGGCGATCCTAGGGGAG
  GGAGGGAAGGACAGCGCCAGGAAGTCATCTTCTACCTCATCATCCGCCGCAAGCCTCTCT
  TCTACCTGGTCAACGTCATTGCCCCATGCATCCTCATCACTCTTCTGGCCATCTTCGTCT
  TCTACCTGCCACCAGATGCAGGAGAGAAGATGGGGCTCTCAATCTTTGCCCTGCTGACCC
  TTACTGTGTTCCTGCTGCTGCTGGCTGACAAAGTACCTGAGACCTCACTATCAGTACCCA
  TTATTATCAAGTACCTCATGTTTACCATGGTCCTCGTCACCTTCTCAGTCATCCTTAGTG
  TCGTGGTTCTCAACCTGCACCACCGCTCACCCCACACCCACCAAATGCCCCTTTGGGTCC
  GTCAGATCTTCATTCACAAACTTCCGCTGTACCTGCGTCTAAAAAGGCCCAAACCCGAGA
  GAGACCTGATGCCGGAGCCCCCTCACTGTTCTTCTCCAGGAAGTGGCTGGGGTCGGGGAA
  CAGATGAATATTTCATCCGGAAGCCGCCAAGTGATTTTCTCTTCCCCAAACCCAATAGGT
  TCCAGCCTGAACTGTCTGCCCCTGATCTGCGGCGATTTATCGATGGTCCAAACCGGGCTG
  TGGCCCTGCTTCCGGAGCTACGGGAGGTCGTCTCCTCTATCAGCTACATCGCTCGACAGC
  TGCAGGAACAGGAGGACCACGATGCGCTGAAGGAGGACTGGCAGTTTGTGGCCATGGTAG
  TGGACCGCCTCTTCCTGTGGACTTTCATCATCTTCACCAGCGTTGGGACCCTAGTCATCT
  TCCTGGACGCCACGTACCACTTGCCCCCTCCAGACCCCTTTCCTTGAAGACTGGAGGGTT
  GAGACCCAGGCCCCCTGCCAGTTGAAGTGAGAGTTTGGTGATACTGTCAAGCCCTATCCT
  TCTCTGCCTCTTAACTCCTTCACGAGGAATCTGGGCCTCTTATTTCGTTTCTGGGGACTG
  CATTGGACTGAGGGCTGGGTAGGCAGGTGTCTTGGACCCACCTGAAATGCAGTATCATCT
  GATTTACTCTTTGGGATCTTGAAGAAGCTCTTTTGGGTATCAACACCTAGGTCGCCAGTG
  AAATAGAACACAGAACAGGAACTAGATTATAAGCCTTATGAGGTCAAGAAATGTGACTTG
  GCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGGAT
  CACCTGAGGTCGGGAGTTTGAGACCAGCCCGACCAACATGGAGAAACCCTGTCTCTACTA
  AAAATACAAAATTAGCCAGGTGTGGTGGTACATGCCTGTAATCCCAGCTACTAGGGAGGC
  TGAGGCAGGAGAATCACTCGAACCCGGGAGGCAGAGGTTGCAGTGAGTCAAGATCACGCC
  ATTGCACTCCAGCCTGGGCAACAAGAGCGAAACTCCATCTCAAAAAAAAAAAAAGTGTCT
  TGTTCACTGCAGCATGCCCAGTGCCACGCACAGGTGCTGACCTATAGTAAGTGCTTAACA
  TTTGTTGAATAGGGGAAAGAAATTTCCGGAAGTAAATACAGCAATTAATAATGTTTATAA
  GCTGGGCATGGTCGCTCAGGCCAGTAATCCCAGTGACTTAGGAGGCTGAGGTGGGAGGAT
  TACTTGAAACCCACAGTTTGAGACCAGCCTGGGCAACATAGTGAGACCCTGTCTCTAAAA
  AAATAAAAATAGAAATAAAGTAGTGCTTATTGTTTGCAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000747
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000747.2, NP_000738.2
• RefSeq Size: 2437 bp
• RefSeq ORF: 1506 bp
• Locus ID: 1140
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Gene Summary: 'The muscle acetylcholine receptor is composed of five subunits: two alpha subunits and one beta, one gamma, and one delta subunit. This gene encodes the beta subunit of the acetylcholine receptor. The acetylcholine receptor changes conformation upon acetylcholine binding leading to the opening of an ion-conducting channel across the plasma membrane. Mutations in this gene are associated with slow-channel congenital myasthenic syndrome. [provided by RefSeq, Jul 2008]'