14-3-3 sigma (SFN) (NM_006142) Human Untagged Clone

CAT#: SC319559

SFN (untagged)-Human stratifin (SFN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SFN
• Synonyms: YWHAS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006142.3 GCAGGAGAGACACAGAGTCCGGCATTGGTCCCAGGCAGCAGTTAGCCCGCCGCCCGCCTG
  TGTGTCCCCAGAGCCATGGAGAGAGCCAGTCTGATCCAGAAGGCCAAGCTGGCAGAGCAG
  GCCGAACGCTATGAGGACATGGCAGCCTTCATGAAAGGCGCCGTGGAGAAGGGCGAGGAG
  CTCTCCTGCGAAGAGCGAAACCTGCTCTCAGTAGCCTATAAGAACGTGGTGGGCGGCCAG
  AGGGCTGCCTGGAGGGTGCTGTCCAGTATTGAGCAGAAAAGCAACGAGGAGGGCTCGGAG
  GAGAAGGGGCCCGAGGTGCGTGAGTACCGGGAGAAGGTGGAGACTGAGCTCCAGGGCGTG
  TGCGACACCGTGCTGGGCCTGCTGGACAGCCACCTCATCAAGGAGGCCGGGGACGCCGAG
  AGCCGGGTCTTCTACCTGAAGATGAAGGGTGACTACTACCGCTACCTGGCCGAGGTGGCC
  ACCGGTGACGACAAGAAGCGCATCATTGACTCAGCCCGGTCAGCCTACCAGGAGGCCATG
  GACATCAGCAAGAAGGAGATGCCGCCCACCAACCCCATCCGCCTGGGCCTGGCCCTGAAC
  TTTTCCGTCTTCCACTACGAGATCGCCAACAGCCCCGAGGAGGCCATCTCTCTGGCCAAG
  ACCACTTTCGACGAGGCCATGGCTGATCTGCACACCCTCAGCGAGGACTCCTACAAAGAC
  AGCACCCTCATCATGCAGCTGCTGCGAGACAACCTGACACTGTGGACGGCCGACAACGCC
  GGGGAAGAGGGGGGCGAGGCTCCCCAGGAGCCCCAGAGCTGAGTGTTGCCCGCCACCGCC
  CCGCCCTGCCCCCTCCAGTCCCCCACCCTGCCGAGAGGACTAGTATGGGGTGGGAGGCCC
  CACCCTTCTCCCCTAGGCGCTGTTCTTGCTCCAAAGGGCTCCGTGGAGAGGGACTGGCAG
  AGCTGAGGCCACCTGGGGCTGGGGATCCCACTCTTCTTGCAGCTGTTGAGCGCACCTAAC
  CACTGGTCATGCCCCCACCCCTGCTCTCCGCACCCGCTTCCTCCCGACCCCAGGACCAGG
  CTACTTCTCCCCTCCTCTTGCCTCCCTCCTGCCCCTGCTGCCTCTGATCGTAGGAATTGA
  GGAGTGTCCCGCCTTGTGGCTGAGAACTGGACAGTGGCAGGGGCTGGAGATGGGTGTGTG
  TGTGTGTGTGTGTGTGTGTGTGTGCGCGCGCGCCAGTGCAAGACCGAGATTGAGGGAAAG
  CATGTCTGCTGGGTGTGACCATGTTTCCTCTCAATAAAGTTCCCCTGTGACACTCCAAAA
  AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006142
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006142.3, NP_006133.1
• RefSeq Size: 1336 bp
• RefSeq ORF: 747 bp
• Locus ID: 2810
• Cytogenetics: 1p36.11
• Domains: 14-3-3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cell cycle, p53 signaling pathway
• Gene Summary: 'This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017]'