PUS1 (NM_025215) Human Untagged Clone

CAT#: SC319608

PUS1 (untagged)-Human pseudouridylate synthase 1 (PUS1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PUS1
• Synonyms: MLASA1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_025215.4 GAGAGGTCAGGGGTCAGCAGGAGTGAGGCTGGGGCGTCCAGGTCCGAGAGGTCAGGGGTC
  AGCTGGAGAGGGGCTGGGGCGCCGGTTTCCCGGAGGTCAGGGGTCAGAAGGAACAGGGCT
  GCAGCGTCAGGGTCCGAGAGGTTAGGGGTCGGCAGAGGCGGAGCTGGGGCACTGGGGGTC
  AGGGGTCGGGGATCAGGGCGGGGTCGGGTGCACTGGTAGCCTGCGCATGGGCCTCCAGCT
  TCGCGCGCTGTTGGGAGCCTTCGGACGGTGGACCCTGCGCCTGGGACCGCGTCCGTCCTG
  CTCGCCGCGCATGGCCGGGAACGCGGAGCCGCCGCCCGCCGGAGCCGCATGCCCCCAGGA
  CCGGAGGTCCTGCAGCGGCCGGGCCGGGGGCGACCGCGTCTGGGAGGACGGAGAACATCC
  GGCGAAGAAGCTCAAGAGCGGTGGCGACGAGGAGCGGCGCGAGAAGCCGCCCAAGCGGAA
  GATCGTGCTGCTCATGGCCTATTCGGGCAAGGGCTACCACGGCATGCAGAGGAATGTCGG
  GTCCTCACAATTCAAAACAATTGAAGATGACTTGGTGTCCGCCCTCGTCCGGTCAGGCTG
  TATTCCTGAAAATCATGGTGAGGACATGAGGAAAATGTCCTTCCAGCGCTGCGCCCGGAC
  AGACAAGGGTGTGTCCGCAGCCGGCCAGGTGGTATCCCTGAAGGTGTGGCTGATTGACGA
  CATTCTAGAAAAGATCAACAGCCACCTTCCCTCTCACATTCGGATTCTGGGACTGAAGCG
  GGTCACGGGCGGGTTTAACTCCAAGAACAGATGTGATGCCAGGACCTATTGCTACCTGCT
  GCCCACGTTTGCCTTTGCGCACAAGGACCGGGACGTTCAGGATGAGACCTACCGCCTGAG
  CGCCGAGACGCTGCAGCAGGTCAACAGGCTCCTGGCCTGCTACAAGGGCACGCACAACTT
  CCACAATTTCACCTCGCAGAAGGGGCCGCAGGATCCCAGTGCCTGCCGCTACATCCTGGA
  GATGTACTGCGAGGAACCCTTTGTGCGGGAGGGCCTGGAGTTTGCGGTGATCAGGGTGAA
  GGGCCAGAGCTTCATGATGCATCAGATCCGGAAGATGGTCGGCCTGGTGGTGGCCATTGT
  GAAGGGTTATGCCCCTGAGAGCGTGCTGGAGCGCAGCTGGGGCACAGAGAAGGTGGACGT
  GCCCAAGGCGCCCGGACTCGGCCTGGTCCTGGAGAGGGTGCACTTCGAGAAGTACAACCA
  GCGCTTTGGCAACGATGGGCTGCATGAGCCGCTGGACTGGGCGCAGGAGGAAGGAAAGGT
  CGCAGCCTTCAAGGAGGAGCACATCTACCCCACCATCATCGGCACCGAGCGGGACGAACG
  CTCCATGGCCCAGTGGCTGAGCACCTTGCCCATCCACAACTTCAGTGCCACCGCTCTCAC
  GGCAGGTGGCACGGGCGCCAAGGTGCCCAGTCCCCTGGAAGGCAGTGAAGGGGACGGAGA
  CACTGACTGAGGCGATGGGAGCTGCCCACCAGAGTGCCTCTGAGCAGCTCACAGTGTGTG
  CCCAGATGTGCCACCCCTGTGGGCAGCAAGAAGCTGGGATCGCTGCAGCCATGTTTTCCC
  GGCCATGCCGGCGTTGTAACCTCAGGACCTTCCCTTGTAGGAACAGCCTTTCTCGAATCT
  GTTTTCAGCTCTTGCATTGCATAGATGAACCTCAGCATGTAAAGAACTATTTTTTTAAAG
  AAGTGATTTTCTTATTAAACAAGTACAAATTTTGCTTAGTCAAAAAAAAAAAAAAAAAAA
  AAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025215.4 unedited
  GCGTATACTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGGCACGAGGGAGAGGTCA
  GGGGTCAGCAGGAGTGAGGCTGGGGCGTCCAGGTCCGAGAGGTCAGGGGTCAGCTGGAGA
  GGGGCTGGGGCGCCGGTTTCCCGGAGGTCAGGGGTCAGAAGGAACAGGGCTGCAGCGTCA
  GGGTCCGAGAGGTTAGGGGTCGGCAGAGGCGGAGCTGGGGCACTGGGGGTCAGGGGTCGG
  GGATCAGGGCGGGGTCGGGTGCACTGGTAGCCTGCGCATGGGCCTCCAGCTTCGCGCGCT
  GTTGGGAGCCTTCGGACGGTGGACCCTGCGCCTGGGACCGCGTCCGTCCTGCTCGCCGCG
  CATGGCCGGGAACGCGGAGCCGCCGCCCGCCGGAGCCGCATGCCCCCAGGACCGGAGGTC
  CTGCAGCGGCCGGGCCGGGGGCGACCGCGTCTGGGAGGACGGAGAACATCCGGCGAAGAA
  GCTCAAGAGCGGTGGCGACGAGGAGCGGCGCGAGAAGCCGCCCAAGCGGAAGATCGTGCT
  GCTCATGGCCTATTCGGGCAAGGGCTACCACGGCATGCAGAGGAATGTCGGGTCCTCACA
  ATTCAAAACAATTGAAGATGACTTGGTGTCCGCCCTCGTCCGGTCAGGCTGTATTCCTGA
  AAATCATGGTGAGGACATGAGGAAAATGTCCTTCCAGCGCTGCGCCCGGACAGACAAGGG
  TGTGTCCGCAGCCGGCCAGGTGGTATCCCTGAAGTGTGGCTGATTGACGACATTCTAGAA
  AGATCAACAGCCACCCTTCCCTCCTCAACATTCGGATTCTGGGACTGAGCGGGTCACGGC
  GGTTAACTCCAGACAGATGTGATGCCAGACTATGCTACTGCTGCCCACGTTGCCTTGCGC
  ACAAGACCGGAACGTTCAGATG
• Restriction Sites: Please inquire
• ACCN: NM_025215
• ORF Size: 1284 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_025215.4, NP_079491.2
• RefSeq Size: 2047
• RefSeq ORF: 1284
• Locus ID: 80324
• Domains: PseudoU_synth_1
• Gene Summary: This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).