ATP6V1C1 (NM_001695) Human Untagged Clone

CAT#: SC319707

ATP6V1C1 (untagged)-Human ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1 (ATP6V1C1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6V1C1
• Synonyms: ATP6C; ATP6D; VATC; Vma5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001695.3 GGGGGGGTAGAGGAAGCCGTGAGGCCGGAGCTTAGGTCGGGAAGGGATGGATCGCTGAGC
  CGATAGCGTCCGCTAGGCTGTCTGCCTCGGTACCTGTTACTGCTGCTACTTCCTCGTTTG
  ACACCTTCCTGGAATCTCTCTTGATTTTTGAGGAAATACCTAGTAACAAACATGACTGAG
  TTCTGGCTTATATCTGCTCCTGGGGAGAAAACCTGTCAGCAAACATGGGAGAAATTGCAT
  GCGGCAACTTCAAAGAACAATAATCTTGCTGTCACTTCCAAGTTCAATATTCCTGACTTA
  AAGGTTGGCACGTTGGATGTCTTGGTTGGCTTGTCAGATGAACTGGCTAAACTGGATGCA
  TTTGTAGAAGGAGTGGTTAAGAAAGTAGCTCAATACATGGCTGATGTATTGGAAGATAGC
  AAAGACAAAGTTCAAGAGAATCTGTTGGCTAATGGAGTGGACTTGGTTACTTATATAACA
  AGGTTCCAGTGGGACATGGCCAAATATCCAATCAAGCAGTCCCTGAAAAATATTTCTGAA
  ATAATTGCCAAGGGAGTAACTCAGATTGATAATGACCTGAAATCTCGAGCATCTGCATAC
  AATAACCTGAAAGGAAATCTTCAGAATTTGGAACGAAAGAATGCAGGAAGTTTGCTAACT
  AGAAGTCTAGCAGAAATTGTGAAGAAGGATGACTTTGTTCTTGATTCAGAGTATCTCGTC
  ACATTACTGGTAGTAGTTCCCAAGTTAAACCACAACGACTGGATTAAGCAGTATGAAACA
  CTAGCCGAAATGGTAGTTCCAAGGTCTAGCAATGTTCTTTCAGAGGACCAAGACAGTTAC
  CTGTGTAATGTCACCTTGTTTAGGAAGGCAGTTGATGACTTCAGACACAAAGCCAGAGAA
  AACAAATTCATTGTTCGTGACTTCCAGTATAATGAAGAGGAGATGAAAGCAGATAAAGAA
  GAAATGAACAGGCTTTCTACTGATAAGAAAAAACAATTTGGACCACTTGTACGGTGGCTG
  AAAGTGAATTTTAGTGAAGCATTTATTGCATGGATTCACGTGAAAGCATTACGGGTTTTC
  GTTGAGTCTGTTTTAAGGTATGGCTTGCCAGTGAACTTCCAAGCAATGCTACTTCAGCCC
  AATAAGAAAACTTTGAAGAAACTGAGAGAAGTATTACATGAATTGTATAAACATCTAGAC
  AGCAGTGCAGCAGCTATTATTGATGCTCCTATGGATATTCCAGGTTTAAACCTGAGTCAA
  CAAGAATACTACCCCTATGTGTACTACAAGATTGATTGCAACTTGCTGGAATTCAAGTGA
  AAATGGGCTCCTCCCCCGACAATCCTGTCCTTGTGTTTGTGTGTGCTAACAGAAATAAGT
  TGCAGTATGGTCGTACTTTTAACTCTAGTATCCTTTGCTTGCTTCTTACCCCCTTTCCTA
  GGTGAATTCTCCCACAGTGGTCTGTATCTCAACATTTTCTTTTTAAAGGAAAAAATATAT
  ATATATAGTTTCTTTTTATTGATCAGGTCTGTAAATGTGTACTAAAAAAATCAGAGTTTA
  TTTATAAACAAAATAGTTTATTTAAAGAGAAGGTCTCTTCCTTATTGATATCATGGTATG
  CATTAATTCCATTTGTTACTATTGTGCACAAAAGCCCTGTTCACAGGGGAATGGTGTAAA
  CATTTATACTGTTTTGTTCACTGTATTTAGTAGACATAACTGTTGAATAGTTACTGAATC
  ATGATGTAAAGAATATGTGACCATCTTCAGGTATGGGATTTCTGAACGTTTCAAATTTCA
  ATCAATGAGCACTGTCAACACCCACAGGAGAGAATAAAATTACCTGTGCAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001695
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001695.3, NP_001686.1
• RefSeq Size: 5721 bp
• RefSeq ORF: 1149 bp
• Locus ID: 528
• Cytogenetics: 8q22.3
• Domains: V-ATPase_C
• Protein Families: Druggable Genome
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: 'This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene is one of two genes that encode the V1 domain C subunit proteins and is found ubiquitously. This C subunit is analogous but not homologous to gamma subunit of F-ATPases. Previously, this gene was designated ATP6D. [provided by RefSeq, Jul 2008]'