ZAK (MAP3K20) (NM_133646) Human Untagged Clone

CAT#: SC320193

ZAK (untagged)-Human sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP3K20
• Synonyms: AZK; CNM6; MLK7; mlklak; MLT; MLTK; MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_133646.2 GCCGCGCGGGCCGCTGTCGTCCCAACCCCCGCCGCCCTCGTCGCGCGCGGGGCCTCCGCG
  CCCCCGGCTGCTGCTCACGCCCCGCCCGGGAGCCAGATTTTGTGGAAGTATAATACTTTG
  TCATTATGAGATGTCGTCTCTCGGTGCCTCCTTTGTGCAAATTAAATTTGATGACTTGCA
  GTTTTTTGAAAACTGCGGTGGAGGAAGTTTTGGGAGTGTTTATCGAGCCAAATGGATATC
  ACAGGACAAGGAGGTGGCTGTAAAGAAGCTCCTCAAAATAGAGAAAGAGGCAGAAATACT
  CAGTGTCCTCAGTCACAGAAACATCATCCAGTTTTATGGAGTAATTCTTGAACCTCCCAA
  CTATGGCATTGTCACAGAATATGCTTCTCTGGGATCACTCTATGATTACATTAACAGTAA
  CAGAAGTGAGGAGATGGATATGGATCACATTATGACCTGGGCCACTGATGTAGCCAAAGG
  AATGCATTATTTACATATGGAGGCTCCTGTCAAGGTGATTCACAGAGACCTCAAGTCAAG
  AAACGTTGTTATAGCTGCTGATGGAGTATTGAAGATCTGTGACTTTGGTGCCTCTCGGTT
  CCATAACCATACAACACACATGTCCTTGGTTGGAACTTTCCCATGGATGGCTCCAGAAGT
  TATCCAGAGTCTCCCTGTGTCAGAAACTTGTGACACATATTCCTATGGTGTGGTTCTCTG
  GGAGATGCTAACAAGGGAGGTCCCCTTTAAAGGTTTGGAAGGATTACAAGTAGCTTGGCT
  TGTAGTGGAAAAAAACGAGAGATTAACCATTCCAAGCAGTTGCCCCAGAAGTTTTGCTGA
  ACTGTTACATCAGTGTTGGGAAGCTGATGCCAAGAAACGGCCATCATTCAAGCAAATCAT
  TTCAATCCTGGAGTCCATGTCAAATGACACGAGCCTTCCTGACAAGTGTAACTCATTCCT
  ACACAACAAGGCGGAGTGGAGGTGCGAAATTGAGGCAACTCTTGAGAGGCTAAAGAAACT
  AGAGCGTGATCTCAGCTTTAAGGAGCAGGAGCTTAAAGAACGAGAAAGACGTTTAAAGAT
  GTGGGAGCAAAAGCTGACAGAGCAGTCCAACACCCCGCTTCTCTTGCCTCTTGCTGCAAG
  AATGTCTGAGGAGTCTTACTTTGAATCTAAAACAGAGGAGTCAAACAGTGCAGAGATGTC
  ATGTCAGATCACAGCAACAAGTAACGGGGAGGGCCATGGCATGAACCCAAGTCTGCAGGC
  CATGATGCTGATGGGCTTTGGGGATATCTTCTCAATGAACAAAGCAGGAGCTGTGATGCA
  TTCTGGGATGCAGATAAACATGCAAGCCAAGCAGAATTCTTCCAAAACCACATCTAAGAG
  AAGGGGGAAGAAAGTCAACATGGCTCTGGGGTTCAGTGATTTTGACTTGTCAGAAGGTGA
  CGATGATGATGATGATGACGGTGAGGAGGAGGATAATGACATGGATAATAGTGAATGAAA
  GCAGAAAGCAAAGTAATAAAATCACAAATGTTTGGAAAACACAAAAAAAAAAAAAAAAAA
  AA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_133646.2 unedited
  GGGCACTTGTAGTACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACC
  GAGGAGATCTGCCGCCGCGATCGCGGCGCGCCAGATCTCAAGCTTAACTAGTTAGCGGAC
  CGACGCGTTAAGCGGCCGCGAATTCAGATCCACAAGTTTGTACAAAAAAGCAGGCTTGTA
  AAACGACGGCCAGTAACTATAACGGTCCTAAGGTAGCGAGGCCTGGGTGGCGAATTCGGC
  ACGAGGGCCGCGCGGGCCGCTGTCGTCCCAACCCCCGCCGCCCTCGTCGCGCGCGGGGCC
  TCCGCGCCCCCGGCTGCTGCTCACGCCCCGCCCGGGAGCCAGATTTTGTGGAAGTATAAT
  ACTTTGTCATTATGAGATGTCGTCTCTCGGTGCCTCCTTTGTGCAAATTAAATTTGATGA
  CTTGCAGTTTTTTGAAAACTGCGGTGGAGGAAGTTTTGGGAGTGTTTATCGAGCCAAATG
  GATATCACAGGACAAGGAGGTGGCTGTAAAGAAGCTCCTCAAAATAGAGAAAGAGGCAGA
  AATACTCAGTGTCCTCAGTCACAGAAACATCATCCAGTTTTATGGAGTAATTCTTGAACC
  TCCCAACTATGGCATTGTCACAGAATATGCTTCTCTGGGATCACTCTATGATTACATTAA
  CAGTAACAGAAGTGAGGAGATGGATATGGATCACATTATGACCTGGGCCACTGATGTAGC
  CAAAGGAATGCATTATTTACATATGGAGGCTCCTGTCAAGGTGATTCACAGAGACCTCAA
  GTCAAGAAACGTTGTTATAGCTGCTGATGGAGTATTGAAGATCTGTGACTTTGGTGCCTC
  TCGGTTCCATAACCATACACACACATGTCCTTGGTTGGAACTTTCCCATGGAATGCTCCA
  GAGTTATCAGAGTCTCCCTGGGTCAGAAACTTGTGAACACAATATTCCCTAATGGTTGTG
  TTTCTCTCTTGGGAGAATGGGC
• Restriction Sites: Please inquire
• ACCN: NM_133646
• ORF Size: 1368 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_133646.2, NP_598407.1
• RefSeq Size: 7194
• RefSeq ORF: 1368
• Locus ID: 51776
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway, Tight junction
• Gene Summary: This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks several exons in the coding region and contains a different 3' exon compared to variant 1. The resulting protein (isoform 2, also referred to as MRK-beta, MLTK-beta and MLK7) is shorter and has a distinct C-terminus compared to isoform 1.