UBE2V1 (NM_001032288) Human Untagged Clone

CAT#: SC320241

UBE2V1 (untagged)-Human ubiquitin-conjugating enzyme E2 variant 1 (UBE2V1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2V1
• Synonyms: CIR1; CROC-1; CROC1; UBE2V; UEV-1; UEV1; UEV1A
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001032288.1 GCGACGCAAGATGGCAGCCACCACGGGCTCGGGAGTAAAAGTCCCTCGCAATTTCCGACT
  GTTGGAAGAACTCGAAGAAGGCCAGAAAGGAGTAGGAGATGGCACAGTTAGCTGGGGTCT
  AGAAGATGACGAAGACATGACACTTACAAGATGGACAGGGATGATAATTGGGCCTCCAAG
  AACAATTTATGAAAACCGAATATACAGCCTTAAAATAGAATGTGGACCTAAATACCCAGA
  AGCACCCCCCTTTGTAAGATTTGTAACAAAAATTAATATGAATGGAGTAAATAGTTCTAA
  TGGAGTGGTGGACCCAAGAGCCATATCAGTGCTAGCAAAATGGCAGAATTCATATAGCAT
  CAAAGTTGTCCTGCAAGAGCTTCGGCGCCTAATGATGTCTAAAGAAAATATGAAACTCCC
  TCAGCCGCCCGAAGGACAGTGTTACAGCAATTAATCAAAAAGAAAAACCACAGGCCCTTC
  CCCTTCCCCCCAATTCGATTTAATCAGTCTTCATTTTCCACAGTAGTAAATTTTCTAGAT
  ACGTCTTGTAGACCTCAAAGTACCGGAAAGGAAGCTCCCATTCAAAGGAAATTTATCTTA
  AGATACTGTAAATGATACTAATTTTTTGTCCATTTGAAATATATAAGTTGTGCTATAACA
  AATCATCCTGTCAAGTGTAACCACTGTCCACGTAGTTGAACTTCTGGGATCAAGAAAGTC
  TATTTAAATTGATTCCCATCATAACTGGTGGGGCACATCTAACTCAACTGTGAAAAGACA
  CATCACACAATCACCTTGCTGCTGATTACACGGCCTGGGGTCTCTGCCTTCTCCCCTTAC
  CCTCCCGCCTCCCACCCTCCCTGCAACAACAGCCCTCTAGCCTGGGGGGCTTGTTAGAGT
  AGATGTGAAGGTTTCAGGTCGCAGCCTGTGGGACTACTGCTAGGTGTGTGGGGTGTTTCG
  CCTGCACCCCTGGTTTCTTTAAGTCTTAAGTGATGCCCCTTCCAAACCATCATCCTGTCC
  CCACGCTCCTCCACTCCCGCCCTTGGCCGAAGCATAGATTGTAACCCCTCCACTCCCCTC
  TGAGATTGGCCTTCGGTGAGGAATTCAGGGCTTTCCCCATATCTTCTCTCCCCCACCTTT
  ATCGAGGGGTGCTGCTTTTTCTCCCTCCTCCTCAAGTTCCTTTTTGCACCGTCACCACCC
  AACACCTTCCATGACACTTCCTTGCTTTGGCCAGAAGCCATCAGGTAAGGTTGGAAAGAG
  CCTCTGACCTCCCTTGTTTAGTTTTGGAACCATACTCACTCACTCTCCACCAGCCTGGGA
  AATGAATATTGGGTCCTCAGCCCTGCCACCCTCTGCTGTCATCAGCTGATGCATTGTTTT
  TAGCTCAGGTTTTGATAAGGTGAAAAGAATAGTCACCAGGGTTACTCAGACCTGCCAGCT
  CTCGGAGTCCTTGGTGGTTGAACTTGGAGAAAGACCGCATGAAGATACTTGTAAGCACAC
  ATGATCCCTCTGAATTGTTTTACTTTCCTGTAACTGCTTTTGCTTTTAAAAATTGAAGAA
  GTTTTAAACAGGGCTTTCATTTGGTCATCCTTGCAATCCATTGGGGTCTAGTTTGGAATC
  TGACAACTGGAACAAAAAGAACCTTGAATCCGGTGCATGCCTTGGTTTTGGTGCTGCTGC
  TGCTTCCCAAGATCCTCAGCAGGGATTAAGAAGGAACCCGGTGTGCACAGCAGATCCCCG
  AAATTGGTGGGCTTGACCTCCTGGCAAATTGCTGCGTCTTTCCACTTGCTGTTCAGGACC
  ACTAAATGCTGAAATGTGGATGCATACCGAAATAAAAGCAATTCATTGTGTAAAAAAAAA
  AAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001032288
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001032288.1, NP_001027459.1
• RefSeq Size: 2138 bp
• RefSeq ORF: 444 bp
• Locus ID: 7335
• Cytogenetics: 20q13.13
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto-oncogene. It is thought to be involved in the control of differentiation by altering cell cycle behavior. Alternatively spliced transcript variants encoding multiple isoforms have been described for this gene, and multiple pseudogenes of this gene have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (Kua-UEV), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Apr 2012]'
  Transcript Variant: This variant (4) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (d) is shorter and has a distinct N-terminus, compared to isoform a.