ATIC (NM_004044) Human Untagged Clone

CAT#: SC320282

ATIC (untagged)-Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATIC
• Synonyms: AICAR; AICARFT; HEL-S-70p; IMPCHASE; PURH
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004044.4 CCGCTCGCCCTGAACCCAGTGCCTGCAGCCATGGCTCCCGGCCAGCTCGCCTTATTTAGT
  GTCTCTGACAAAACCGGCCTTGTGGAATTTGCAAGAAACCTGACCGCTCTTGGTTTGAAT
  CTGGTCGCTTCCGGAGGGACTGCAAAAGCTCTCAGGGATGCTGGTCTGGCAGTCAGAGAT
  GTCTCTGAGTTGACGGGATTTCCTGAAATGTTGGGGGGACGTGTGAAAACTTTGCATCCT
  GCAGTCCATGCTGGAATCCTAGCTCGTAATATTCCAGAAGATAATGCTGACATGGCCAGA
  CTTGATTTCAATCTTATAAGAGTTGTTGCCTGCAATCTCTATCCCTTTGTAAAGACAGTG
  GCTTCTCCAGGTGTAACTGTTGAGGAGGCTGTGGAGCAAATTGACATTGGTGGAGTAACC
  TTACTGAGAGCTGCAGCCAAAAACCACGCTCGAGTGACAGTGGTGTGTGAACCAGAGGAC
  TATGTGGTGGTGTCCACGGAGATGCAGAGCTCCGAGAGTAAGGACACCTCCTTGGAGACT
  AGACGCCAGTTAGCCTTGAAGGCATTCACTCATACGGCACAATATGATGAAGCAATTTCA
  GATTATTTCAGGAAACAGTACAGCAAAGGCGTATCTCAGATGCCCTTGAGATATGGAATG
  AACCCACATCAGACCCCTGCCCAGCTGTACACACTGCAGCCCAAGCTTCCCATCACAGTT
  CTAAATGGAGCCCCTGGATTTATAAACTTGTGCGATGCTTTGAACGCCTGGCAGCTGGTG
  AAGGAACTCAAGGAGGCTTTAGGTATTCCAGCCGCTGCCTCTTTCAAACATGTCAGCCCA
  GCAGGTGCTGCTGTTGGAATTCCACTCAGTGAAGATGAGGCCAAAGTCTGCATGGTTTAT
  GATCTCTATAAAACCCTCACACCCATCTCAGCGGCATATGCAAGAGCAAGAGGGGCTGAT
  AGGATGTCTTCATTTGGTGATTTTGTTGCATTGTCCGATGTTTGTGATGTACCAACTGCA
  AAAATTATTTCCAGAGAAGTATCTGATGGTATAATTGCCCCAGGATATGAAGAAGAAGCC
  TTGACAATACTTTCCAAAAAGAAAAATGGAAACTATTGTGTCCTTCAGATGGACCAATCT
  TACAAACCAGATGAAAATGAAGTTCGAACTCTCTTTGGTCTTCATTTAAGCCAGAAGAGA
  AATAATGGTGTCGTCGACAAGTCATTATTTAGCAATGTTGTTACCAAAAATAAAGATTTG
  CCAGAGTCTGCCCTCCGAGACCTCATCGTAGCCACCATTGCTGTCAAGTACACTCAGTCT
  AACTCTGTGTGCTACGCCAAGAACGGGCAGGTTATCGGCATTGGAGCAGGACAGCAGTCT
  CGTATACACTGCACTCGCCTTGCAGGAGATAAGGCAAACTATTGGTGGCTTAGACACCAT
  CCACAAGTGCTTTCGATGAAGTTTAAAACAGGAGTGAAGAGAGCAGAAATCTCCAATGCC
  ATCGATCAATATGTGACTGGAACCATTGGCGAGGATGAAGATTTGATAAAGTGGAAGGCA
  CTGTTTGAGGAAGTCCCTGAGTTACTCACTGAGGCAGAGAAGAAGGAATGGGTTGAGAAA
  CTGACTGAAGTTTCTATCAGCTCTGATGCCTTCTTCCCTTTCCGAGATAACGTAGACAGA
  GCTAAAAGGAGTGGTGTGGCGTACATTGCGGCTCCCTCCGGTTCTGCTGCTGACAAAGTT
  GTGATTGAGGCCTGCGACGAACTGGGAATCATCCTCGCTCATACGAACCTTCGGCTCTTC
  CACCACTGATTTTACCACACACTGTTTTTTGGCTTGCTTATGTGTAGGTGAACAGTCACG
  CCTGAAACTTTGAGGATAACTTTTTAAAAAAATAAAACAGTATCTCTTAAAAAAAAAAAA
  AAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004044
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004044.4, NP_004035.2
• RefSeq Size: 1936 bp
• RefSeq ORF: 1779 bp
• Locus ID: 471
• Cytogenetics: 2q35
• Domains: AICARFT_IMPCHas, MGS
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Metabolic pathways, One carbon pool by folate, Purine metabolism
• Gene Summary: 'This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. [provided by RefSeq, Sep 2009]'