GJA5 (NM_005266) Human Untagged Clone

CAT#: SC320311

GJA5 (untagged)-Human gap junction protein, alpha 5, 40kDa (GJA5), transcript variant A

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GJA5
• Synonyms: ATFB11; CX40
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005266.5 CCATTTTCAAACAGTCCCTCCTGGGAGAACACAGACAGGCAGAGGATTACAACACAAGGC
  AGCAAGCACTGGGAGACGAAAGTTTTGGCATCTGTTCCCTGGCTGTGCCAAGATGGGCGA
  TTGGAGCTTCCTGGGAAATTTCCTGGAGGAAGTACACAAGCACTCGACCGTGGTAGGCAA
  GGTCTGGCTCACTGTCCTCTTCATATTCCGTATGCTCGTGCTGGGCACAGCTGCTGAGTC
  TTCCTGGGGGGATGAGCAGGCTGATTTCCGGTGTGATACGATTCAGCCTGGCTGCCAGAA
  TGTCTGCTACGACCAGGCTTTCCCCATCTCCCACATTCGCTACTGGGTGCTGCAGATCAT
  CTTCGTCTCCACGCCCTCTCTGGTGTACATGGGCCACGCCATGCACACTGTGCGCATGCA
  GGAGAAGCGCAAGCTACGGGAGGCCGAGAGGGCCAAAGAGGTCCGGGGCTCTGGCTCTTA
  CGAGTACCCGGTGGCAGAGAAGGCAGAACTGTCCTGCTGGGAGGAAGGGAATGGAAGGAT
  TGCCCTCCAGGGCACTCTGCTCAACACCTATGTGTGCAGCATCCTGATCCGCACCACCAT
  GGAGGTGGGCTTCATTGTGGGCCAGTACTTCATCTACGGAATCTTCCTGACCACCCTGCA
  TGTCTGCCGCAGGAGTCCCTGTCCCCACCCGGTCAACTGTTACGTATCCCGGCCCACAGA
  GAAGAATGTCTTCATTGTCTTTATGCTGGCTGTGGCTGCACTGTCCCTCCTCCTTAGCCT
  GGCTGAACTCTACCACCTGGGCTGGAAGAAGATCAGACAGCGATTTGTCAAACCGCGGCA
  GCACATGGCTAAGTGCCAGCTTTCTGGCCCCTCTGTGGGCATAGTCCAGAGCTGCACACC
  ACCCCCCGACTTTAATCAGTGCCTGGAGAATGGCCCTGGGGGAAAATTCTTCAATCCCTT
  CAGCAATAATATGGCCTCCCAACAAAACACAGACAACCTGGTCACCGAGCAAGTACGAGG
  TCAGGAGCAGACTCCTGGGGAAGGTTTCATCCAGGTTCGTTATGGCCAGAAGCCTGAGGT
  GCCCAATGGAGTCTCACCAGGTCACCGCCTTCCCCATGGCTATCATAGTGACAAGCGACG
  TCTTAGTAAGGCCAGCAGCAAGGCAAGGTCAGATGACCTATCAGTGTGACCCTCCTTTAT
  GGGAGGATCAGGACCAGGTGGGAACAAAGGAGGCTCAGAGAGGAAAGACGTGTCCCTTCT
  GAACTGATGCTTTCTCACTGTCATCACTGCTTGGCTCCTTTGAGCCCCGGGTCTCAATGA
  CGTTGCTCATTAATTCTAGAAACTATAACCAGGGCTCTGGGATAGTAAGAGAGGTGACAA
  CCCACCCAGACTGCAGTTCCCTCCCCACCCTCTACCCAGTATACGAAGCCTTTCAGATTA
  CTCATGAAACAGGGTAGAGGGAAAGAAGGGAAGCATGGCAAAAGCTGGCCTGGAAGGGAT
  AGCCAGAGGGATAGAATGACTCTCTCTCTACATACCAGCAGCATACCAAATGCGTTCTCT
  AAGTTCCTACCTCCTTGACCTGATCACCCTCCCTCCTCCAAGGAAGAGCTCAAAGTTCCC
  AGCCAATAGACAGCATGAATCAAGGAACTTGCATTATATGTGCTCTTGAATCTGTTGTCT
  CCATGGACCATTCCTCGGAGTAGTGGTGAGATGGCCTTGGGTTGCCCTTGGCTTCTCCTC
  CCTCTACTCAGCCTTAAAAAGGGCTTCTTGGAACTTTACCAGCAGCCTCAGCTTTACAAA
  TGCCTTGGTATGTACCTCTGGCAAATGCCCTGGTATGTACCTCTGGCAAATGCCCCACCT
  TGGTGATGTTGCAACCTTTCCTTCTGCTAGGGTGTACACCTAGCCTGTGCAGGTGTCAGC
  CCTGCTAGGGAGTCACTGTACACACAAACTCTACTGGAATTCCTGCCAACATCTGTCACC
  CTGCAGCTCCTTTACAGTTCAATCCAATGATAGAAACCATCCCTTCCCTTTCTCCCTTGG
  CTGTTCACCCAGCCATTCCCTGAAGGCCTTACCAACAGGAATATCCAAGAAGCTGTTGTC
  CTCTCTCGAACCCTGACCAGATCATCAGCCACTGAGGCCAGTGGAATTTCCCCAGGCCTT
  GTTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005266
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005266.5, NP_005257.2
• RefSeq Size: 3177 bp
• RefSeq ORF: 1077 bp
• Locus ID: 2702
• Cytogenetics: 1q21.2
• Domains: CNX
• Protein Families: Transmembrane
• Gene Summary: 'This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (A) represents the longer transcript. Variants A and B encode the same protein.