GPT2 (NM_133443) Human Untagged Clone

CAT#: SC320514

GPT2 (untagged)-Human glutamic pyruvate transaminase (alanine aminotransferase) 2 (GPT2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPT2
• Synonyms: ALT2; GPT 2; MRT49
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_133443.1 CTAACCGAGTGCGGCGAGGGCCTACCAGGGGCGACAGGGTTTCTCTCCGCAAGCGCGCGA
  TGCAGCGGGCGGCGGCGCTGGTCCGGCGGGGCTGTGGTCCCCGGACCCCCAGCTCCTGGG
  GCCGCAGCCAGAGCAGCGCGGCCGCCGAGGCCTCGGCGGTGCTCAAGGTGCGGCCCGAGC
  GCAGCCGGCGCGAGCGCATCCTCACGCTGGAGTCCATGAACCCGCAGGTGAAGGCGGTGG
  AGTACGCCGTGCGGGGACCCATCGTGCTCAAGGCCGGCGAGATCGAGCTCGAGCTGCAGC
  GGGGTATCAAAAAGCCATTCACAGAGGTCATCCGAGCCAACATCGGGGACGCCCAGGCTA
  TGGGGCAGCAGCCAATCACCTTCCTCCGGCAGGTGATGGCACTATGCACCTACCCAAACC
  TGCTGGACAGCCCCAGCTTCCCAGAAGATGCTAAGAAACGTGCCCGGCGGATCCTGCAGG
  CTTGTGGCGGGAACAGCCTGGGGTCCTACAGTGCTAGCCAGGGTGTCAACTGCATCCGTG
  AAGATGTGGCTGCCTACATCACCAGGAGGGATGGCGGTGTGCCTGCGGACCCCGACAACA
  TCTACCTGACCACGGGAGCTAGTGACGGCATTTCTACGATCCTGAAGATCCTCGTCTCCG
  GGGGCGGCAAGTCACGGACAGGTGTGATGATCCCCATCCCACAATATCCCCTCTATTCAG
  CTGTCATCTCTGAGCTCGACGCCATCCAGGTGAATTACTACCTGGACGAGGAGAACTGCT
  GGGCGCTGAATGTGAATGAGCTCCGGCGGGCGGTGCAGGAGGCCAAAGACCACTGTGATC
  CTAAGGTGCTCTGCATAATCAACCCTGGGAACCCCACAGGCCAGGTACAAAGCAGAAAGT
  GCATAGAAGATGTGATCCACTTTGCCTGGGAAGAGAAGCTCTTTCTCCTGGCTGATGAGG
  TGTACCAGGACAACGTGTACTCTCCAGATTGCAGATTCCACTCCTTCAAGAAGGTGCTGT
  ACGAGATGGGGCCCGAGTACTCCAGCAACGTGGAGCTCGCCTCCTTCCACTCCACCTCCA
  AGGGCTACATGGGCGAGTGTGGTTACAGAGGAGGCTACATGGAGGTGATCAACCTGCACC
  CTGAGATCAAGGGCCAGCTGGTGAAGCTGCTGTCGGTGCGCCTGTGCCCCCCAGTGTCTG
  GGCAGGCCGCCATGGACATTGTCGTGAACCCCCCGGTGGCAGGAGAGGAGTCCTTTGAGC
  AATTCAGCCGAGAGAAGGAGTCGGTCCTGGGTAATCTGGCCAAAAAAGCAAAGCTGACGG
  AAGACCTGTTTAACCAAGTCCCAGGAATTCACTGCAACCCCTTGCAGGGGGCCATGTACG
  CCTTCCCTCGGATCTTCATTCCTGCCAAAGCTGTGGAGGCTGCTCAGGCCCATCAAATGG
  CTCCAGACATGTTCTACTGCATGAAGCTCCTGGAGGAGACTGGCATCTGTGTCGTGCCCG
  GCAGTGGCTTTGGGCAGAGGGAAGGCACTTACCACTTCAGGATGACTATCCTCCCTCCAG
  TGGAGAAGCTGAAAACGGTGCTGCAGAAGGTGAAAGACTTCCACATCAACTTCCTGGAGA
  AGTACGCGTGAGGACGCCTGAGCCCCAGCGGGAGACCTGTCCTTGGCTCTTCCTCCCAAT
  GCCCGTCAGGCTGAACTCGCCTCCCCCGTGACTCTGCCTCGGGCCTCGCAGAGGCCGCTG
  GTCACTTCGTCATCATTTTGCCCCTGGAGACGTCTTTCTTTGTGCCTTGATGTTGAGAGC
  GCCTCTCTTTTGAGCAAACAAGCATTCTATATGCAACCAGAGTAGAGGGGACCTGCTCAG
  CAGGTGTGACCAGGGTTCTCTGAATCTGTTATTGTTTTTGCTTCTGGAAAGTTCATTTGG
  GGTTTACAACAACTAGGATGTGTTGGGTGAGATGTTTCAGATCTGGAGAAATGAGCAGGT
  GTCGGGAAATGTGTGACTTAACCGTGGTGAGGGCTGGAAATCCAAACTCACCACCATGAT
  CTGTGAAATAAAGCCCTTAGCGGTGTGAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_133443.1 unedited
  TTAAGCGACACGAGTACTACTACGATATATGTATATATCTTCAGGCGGTGGCAGCACACT
  AGGCCGGGAATTCGTCGACTACTAGTTAGCGGACCGACGCGTTAAGCGGCCGCGAATTCA
  GATCCACAAGTTTGTACAAAAAAGCAGGCTTGTAAAACGACGGCCAGTAACTATAACGGT
  CCTAAGGTAGCGAGGCCTGGGTGGCGAATTCGGCACGAGGCTAACCGAGTGCGGCGAGGG
  CCTACCAGGGGCGACAGGGTTTCTCTCCGCAAGCGCGCGATGCAGCGGGCGGCGGCGCTG
  GTCCGGCGGGGCTGTGGTCCCCGGACCCCCAGCTCCTGGGGCCGCAGCCAGAGCAGCGCG
  GCCGCCGAGGCCTCGGCGGTGCTCAAGGTGCGGCCCGAGCGCAGCCGGCGCGAGCGCATC
  CTCACGCTGGAGTCCATGAACCCGCAGGTGAAGGCGGTGGAGTACGCCGTGCGGGGACCC
  ATCGTGCTCAAGGCCGGCGAGATCGAGCTCGAGCTGCAGCGGGGTATCAAAAAGCCATTC
  ACAGAGGTCATCCGAGCCAACATCGGGGACGCCCAGGCTATGGGGCAGCAGCCAATCACC
  TTCCTCCGGCAGGTGATGGCACTATGCACCTACCCAAACCTGCTGGACAGCCCCAGCTTC
  CCAGAAGATGCTAAGAAACGTGCCCGGCGGATCCTGCAGGCTTGTGGCGGGAACAGCCTG
  GGGTCCTACAGTGCTAGCCAGGGTGTCAACTGCATCCGTGAAGATGTGGCTGCCTACATC
  ACCAGAGGGATGGCGTGTGCCTGCGGACCCCGACAACATCTACCTGACCACGGGAGCTAG
  TGACGGCATTTCTACGATCCTGAAGATCCTCGTCTCCGGGGGGCGGCAGTCCACGGACAG
  GTGTTGAATGAATCCCCATTCCACAATATTCCCCCCTCCAAATC
• Restriction Sites: Please inquire
• ACCN: NM_133443
• ORF Size: 1572 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_133443.1, NP_597700.1
• RefSeq Size: 3957
• RefSeq ORF: 1572
• Locus ID: 84706
• Domains: aminotran_1_2
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways
• Gene Summary: This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).