GRK6 (NM_001004106) Human Untagged Clone

CAT#: SC320538

GRK6 (untagged)-Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRK6
• Synonyms: GPRK6
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001004106.1 CCGAGCCGCGCCGAGCCGCGCCGATCGCCATCCGGCCTCGGCACTCGCGCGCGATCCCGG
  CCGGCGGCGCGGCCCGGCGGGCCAGGCGGCGCCACAGCCCATGGAGCTCGAGAACATCGT
  AGCGAACACGGTGCTACTCAAGGCCCGGGAAGGTGGCGGTGGAAATCGCAAAGGCAAAAG
  CAAGAAATGGCGGCAGATGCTCCAGTTCCCTCACATCAGCCAGTGCGAAGAGCTGCGGCT
  CAGCCTCGAGCGTGACTATCACAGCCTGTGCGAGCGGCAGCCCATTGGGCGCCTGCTGTT
  CCGAGAGTTCTGTGCCACGAGGCCGGAGCTGAGCCGCTGCGTCGCCTTCCTGGATGGGGT
  GGCCGAGTATGAAGTGACCCCGGATGACAAGCGGAAGGCATGTGGGCGGCAGCTAACGCA
  GAATTTTCTGAGCCACACGGGTCCTGACCTCATCCCTGAGGTCCCCCGGCAGCTGGTGAC
  GAACTGCACCCAGCGGCTGGAGCAGGGTCCCTGCAAAGACCTTTTCCAGGAACTCACCCG
  GCTGACCCACGAGTACCTGAGCGTGGCCCCTTTTGCCGACTACCTCGACAGCATCTACTT
  CAACCGTTTCCTGCAGTGGAAGTGGCTGGAAAGGCAGCCAGTGACCAAAAACACCTTCAG
  GCAATACCGAGTCCTGGGCAAAGGTGGCTTTGGGGAGGTGTGCGCCTGCCAGGTGCGGGC
  CACAGGTAAGATGTATGCCTGCAAGAAGCTAGAGAAAAAGCGGATCAAGAAGCGGAAAGG
  GGAGGCCATGGCGCTGAACGAGAAGCAGATCCTGGAGAAAGTGAACAGTAGGTTTGTAGT
  GAGCTTGGCCTACGCCTATGAGACCAAGGACGCGCTGTGCCTGGTGCTGACACTGATGAA
  CGGGGGCGACCTCAAGTTCCACATCTACCACATGGGCCAGGCTGGCTTCCCCGAAGCGCG
  GGCCGTCTTCTACGCCGCCGAGATCTGCTGTGGCCTGGAGGACCTGCACCGGGAGCGCAT
  CGTGTACAGGGACCTGAAGCCCGAGAACATCTTGCTGGATGACCACGGCCACATCCGCAT
  CTCTGACCTGGGACTAGCTGTGCATGTGCCCGAGGGCCAGACCATCAAAGGGCGTGTGGG
  CACCGTGGGTTACATGGCTCCGGAGGTGGTGAAGAATGAACGGTACACGTTCAGCCCTGA
  CTGGTGGGCGCTCGGCTGCCTCCTGTACGAGATGATCGCAGGCCAGTCGCCCTTCCAGCA
  GAGGAAGAAGAAGATCAAGCGGGAGGAGGTGGAGCGGCTGGTGAAGGAGGTCCCCGAGGA
  GTATTCCGAGCGCTTTTCCCCGCAGGCCCGCTCACTTTGCTCACAGCTCCTCTGCAAGGA
  CCCTGCCGAACGCCTGGGGTGTCGTGGGGGCAGTGCCCGCGAGGTGAAGGAGCACCCCCT
  CTTTAAGAAGCTGAACTTCAAGCGGCTGGGAGCTGGCATGCTGGAGCCGCCGTTCAAGCC
  TGACCCCCAGGCCATTTACTGCAAGGATGTTCTGGACATTGAACAGTTCTCTACGGTCAA
  GGGCGTGGAGCTGGAGCCTACCGACCAGGACTTCTACCAGAAGTTTGCCACAGGCAGTGT
  GCCCATCCCCTGGCAGAACGAGATGGTGGAGACCGAGTGCTTCCAAGAGCTGAATGTCTT
  TGGGCTGGATGGCTCAGTTCCCCCAGACCTGGACTGGAAGGGCCAGCCACCTGCACCTCC
  TAAAAAGGGACTGCTGCAGAGACTCTTCAGTCGCCAAGATTGCTGTGGAAACTGCAGCGA
  CAGCGAGGAAGAGCTCCCCACCCGCCTCTAGCCCCCAGCCCGAGGCCCCCACCAGCAGTT
  GGCGGTAGCAGCTACTCCGAGCGCCGTTTACAGTTTTGCACAGTGATCTTCCCCATTGTC
  CACTCAAGTCGTGGCCTGGGGAACACAGACGGAGCTGTCCCCAGTGTCCTCCGTCCCTCA
  GCCCCTGGCCTGGCTGAGTTTGGCAGGGCCTGGGCCATCCCTGGGACAAAGGTGCGTCCC
  TTCAGCTCTTCTCCGTGGAGCTCGGGGCTTTCTGTATTTATGTATTTGTACGAATGTATA
  TAGCGACCAGAGCATTCTTAATTCCCGCCGCAGACCTGGCGCCCCCGCCTTGGCTCCTGG
  GGGCAGCCAGCCCTGGCTGGGAGAGCGGGAGCTGGCAGAGGAGCCACTGCCAAACTCAAG
  GCTCCTCTGGCCCAGCTTGGATGGCTGAGGGTGGTCACACCCCTGAGCCTTCAGCACTGT
  GCTGGCCACCCCGGCCTCTGAGTAAGACTCGTGCCTCCCCCTGCTGCCCTGGGCTCAGGC
  TGCTACCCTCTGGGGCCCAAAGCTGTCCCTTCTCAGTGCTTGTCAGCGCTGGGTCTGGGG
  CCTCTGTATGCCCTAGGCCTGTGCCAAAGTGGCCAGAGATTGGGCTGCCTGTGATACCCA
  TCAGCCCACTGCCCCGGCCGGCCCAGATAGGTCTGCCTCTGCCTTCCAGCTCCCACAGCC
  TGGTCCCTGATACTGGGCTCTGTCCTGCAGACACCTCTTTCAGAAACGCCCAAGCCCAGC
  CCCTAGGAGGGGGTGGGGCATCCCTGGTCAACCCTCAAACATTCCGGACTCCCCTCATAA
  CAATAGACACATGTGCCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001004106
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004106.1, NP_001004106.1
• RefSeq Size: 2930 bp
• RefSeq ORF: 1731 bp
• Locus ID: 2870
• Cytogenetics: 5q35.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• Gene Summary: 'This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) uses an alternate acceptor splice site in the last exon, compared to variant 2, resulting in a shorter isoform (A) compared to isoform B.