Phosphoribosyl pyrophosphate amidotransferase (PPAT) (NM_002703) Human Untagged Clone

CAT#: SC320704

PPAT (untagged)-Human phosphoribosyl pyrophosphate amidotransferase (PPAT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPAT
• Synonyms: ATASE; GPAT; PRAT
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002703.3 GCAGGTCCTTCCACGTGCTTTCGGCGGCGACATGGAGCTGGAGGAGTTGGGGATCCGAGA
  GGAATGTGGCGTGTTCGGGTGCATCGCCTCAGGAGAGTGGCCCACGCAGCTGGATGTACC
  GCATGTGATCACTCTGGGACTCGTGGGGCTGCAGCACCGGGGTCAGGAGAGTGCTGGTAT
  TGTGACTAGTGATGGGAGTTCCGTGCCAACATTCAAATCACACAAGGGAATGGGTCTTGT
  AAATCACGTCTTTACTGAAGACAATTTGAAAAAATTATATGTTTCAAATCTTGGAATTGG
  ACACACCAGGTATGCCACCACAGGAAAATGTGAACTAGAAAATTGTCAGCCCTTCGTTGT
  TGAAACACTTCATGGGAAGATAGCTGTGGCACATAATGGCGAATTGGTAAATGCTGCTCG
  ATTAAGGAAAAAGCTTCTGCGTCATGGTATTGGTCTGTCTACAAGTTCTGATAGTGAAAT
  GATTACCCAGTTACTGGCGTATACCCCTCCTCAGGAACAAGATGACACCCCAGACTGGGT
  AGCCAGGATTAAAAACTTGATGAAGGAAGCACCCACAGCATACTCCCTGCTTATAATGCA
  CAGAGATGTTATTTATGCAGTACGAGATCCTTATGGAAATCGTCCCTTATGCATTGGTCG
  TCTTATTCCAGTGTCTGATATAAATGACAAAGAGAAAAAAACATCAGAAACAGAAGGATG
  GGTGGTGTCTTCAGAATCTTGTAGCTTCTTATCTATTGGTGCAAGATATTACCGTGAAGT
  CTTGCCTGGAGAAATTGTGGAAATATCCAGACACAATGTCCAAACTCTTGATATTATATC
  AAGGTCTGAAGGAAACCCAGTGGCTTTTTGTATCTTTGAATATGTTTATTTTGCAAGACC
  AGACAGTATGTTCGAAGACCAAATGGTTTATACAGTAAGATACCGTTGTGGCCAGCAGCT
  AGCGATTGAAGCACCTGTGGATGCAGATTTGGTTAGCACTGTTCCAGAATCTGCTACGCC
  TGCTGCTCTTGCTTACGCAGGAAAGTGTGGACTTCCATATGTGGAGGTGCTGTGTAAAAA
  CCGGTATGTAGGGAGAACCTTCATTCAGCCAAACATGAGGTTAAGACAACTTGGTGTTGC
  AAAAAAATTTGGAGTATTGTCAGACAACTTTAAAGGCAAAAGAATTGTTCTTGTAGATGA
  TTCAATTGTCAGAGGCAATACCATCTCACCTATAATAAAACTGCTCAAAGAATCTGGTGC
  AAAAGAGGTACACATTCGAGTAGCTTCACCACCAATTAAATATCCATGCTTCATGGGAAT
  AAACATTCCTACAAAAGAAGAGCTCATTGCCAATAAACCAGAATTTGATCACCTTGCAGA
  ATATCTAGGAGCAAACAGTGTTGTGTATCTGTCAGTAGAAGGACTGGTTTCATCTGTACA
  AGAAGGGATAAAGTTTAAAAAACAGAAAGAGAAAAAGCACGATATTATGATCCAAGAAAA
  TGGAAATGGTCTGGAATGTTTTGAAAAGAGTGGTCATTGTACAGCTTGTCTCACTGGAAA
  ATATCCTGTAGAATTAGAATGGTAGCTGGTAGGGTTGGATGTGTGTAGTTTCAAGATAGA
  AAGTTGGTCAAGAAGTTATAGTGGTCACACCTCATCTATTTACTGTTACTCAGTTGGTAC
  AATGTAAAATGCCATGCTTATGTTTATAAGTTTTGAGATTTTTTTTTTTTCTGAAAAGGA
  TACCAAAGTGCGATAACTGAACATTTCCAATTGCATATAATACAACAATATGTGGTGTTC
  TTTTTTTTACACAAGCATTGGCTAGCCTTTTTAACCTGGTCAGAGAAGGCAGGTGGTCAC
  TGACATTTCCCAAGTCCATGCTTTAAAGGGTTTGCAAGAAGTTAGGGTTAAGGAGAGGTG
  ATGCCAACAAGACAGGTGAGTTAAATATACCATTTCACACAAAGTTTGAATAGAATACAT
  TATACCTCATAGGTGTCTAGCCTCTACAGTTCTGGCTGTAGTTATGACCTTGGCTTCCCT
  GTCTAACTGTAGACAAATCTTTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002703
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002703.3, NP_002694.3
• RefSeq Size: 3756 bp
• RefSeq ORF: 1554 bp
• Locus ID: 5471
• Cytogenetics: 4q12
• Domains: GATase_2, Pribosyltran
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism
• Gene Summary: 'The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]'
  Transcript Variant: This variant (1) represents the longer transcript.