RFC4 (NM_002916) Human Untagged Clone

CAT#: SC320866

RFC4 (untagged)-Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFC4
• Synonyms: A1; RFC37
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002916.3 GGACATCAGTGATCGTAAGTCTCCTGGGCCCGTTATTCTCAGATTAGGTGACGGAGCTAA
  GACTTCGAGACCATCTCGTCCTTTTTGTATCGCGGAAACCTGAGGAACGAGCCGGCGGCG
  GTGACCTGCACGAGAAGCCAGGCTAACTGGGTGAAGTACCATGCAAGCATTTCTTAAAGG
  TACATCCATCAGTACTAAACCCCCGCTGACCAAGGATCGAGGAGTAGCTGCCAGTGCGGG
  AAGTAGCGGAGAGAACAAGAAAGCCAAACCCGTTCCCTGGGTGGAAAAATATCGCCCAAA
  ATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCAGTGCTGAAAAAATCTTTAGA
  AGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCTGGAACTGGAAAAACATCCAC
  TATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAACTTTTCCGATTAAGAGTTCTTGA
  GTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTCGAGAGAAAGTGAAAAATTTTGC
  TCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGCCGTGTCCGCCTTTTAAGATTGT
  GATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAGGCAGCTTTAAGACGTACCAT
  GGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGTAACTATGTCAGTCGAATAAT
  TGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCAAGCCTCTGTCAGATAAAATTCA
  ACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATGTCAAAATTAGTGATGAGGGAAT
  AGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAAAAGCCATTACATTTCTTCAAAG
  CGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAAGTGATTACAGACATTGCTGG
  GGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCCTGTCAGAGTGGCTCTTTTGA
  CAAACTAGAAGCTGTGGTCAAGGATTTAATAGATGAGGGTCATGCAGCAACTCAGCTCGT
  CAATCAACTCCATGATGTGGTTGTAGAAAATAACTTATCTGATAAACAGAAGTCTATTAT
  CACAGAAAAACTTGCCGAAGTTGACAAATGCCTAGCAGATGGTGCTGATGAACATTTGCA
  ACTCATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCTCAGAATTGTTAACGTGAATA
  TATCTGGATGGGGGGTTTTGTAAATAATGAAGTTGTAATAAAAATAAAATGACCCAAACC
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002916
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002916.3, NP_002907.1
• RefSeq Size: 1427 bp
• RefSeq ORF: 1092 bp
• Locus ID: 5984
• Cytogenetics: 3q27.3
• Domains: AAA, AAA
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair
• Gene Summary: 'The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform.