RPA34 (RPA2) (NM_002946) Human Untagged Clone

CAT#: SC320940

RPA2 (untagged)-Human replication protein A2, 32kDa (RPA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPA2
• Synonyms: REPA2; RP-A p32; RP-A p34; RPA32
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002946.3 CTTCGTAAAGATGGCCGCGGAGGCTTTTGGAGCCAACTGGGAGCGCAGTACGCGTTTTCT
  GGAGCATGGGCAGAGGAGACAGGAACAAGCGTAGCATCCGTGAGCACCGATTGGCTGAAG
  CGAGCACCCCGGGAGCTGACTGGCTCCGCCATTCGCGGGAAGGCGTTTGTGGTGCCAGAG
  AAAAGTAGCCAGAGCGGCGCAGTGGCGGCCGCGTTCTGTGGTTTTCCGCTATTCCCCCAG
  ACCCGCACCTTCTCGGCCTCTTTGCGGAGAATCGTGACCAAGATGTGGAACAGTGGATTC
  GAAAGCTATGGCAGCTCCTCATACGGGGGAGCCGGCGGCTACACGCAGTCCCCGGGGGGC
  TTTGGATCGCCCGCACCTTCTCAAGCCGAAAAGAAATCAAGAGCCCGAGCCCAGCACATT
  GTGCCCTGTACTATATCTCAGCTGCTTTCTGCCACTTTGGTTGATGAAGTGTTCAGAATT
  GGGAATGTTGAGATTTCACAGGTCACTATTGTGGGGATCATCAGACATGCAGAGAAGGCT
  CCAACCAACATTGTTTACAAAATAGATGACATGACAGCTGCACCCATGGACGTTCGCCAG
  TGGGTTGACACAGATGACACCAGCAGTGAAAACACTGTGGTTCCTCCAGAAACATATGTG
  AAAGTGGCAGGCCACCTGAGATCTTTTCAGAACAAAAAGAGCCTGGTAGCCTTTAAGATC
  ATGCCCCTGGAGGATATGAATGAGTTCACCACACATATTCTGGAAGTGATCAATGCACAC
  ATGGTACTAAGCAAAGCCAACAGCCAGCCCTCAGCAGGGAGAGCACCTATCAGCAATCCA
  GGAATGAGTGAAGCAGGGAACTTTGGTGGGAATAGCTTCATGCCAGCAAATGGCCTCACT
  GTGGCCCAAAACCAGGTGTTGAATTTGATTAAGGCTTGTCCAAGACCTGAAGGGTTGAAC
  TTTCAGGATCTCAAGAACCAGCTGAAACACATGTCTGTATCCTCAATCAAGCAAGCTGTG
  GATTTTCTGAGCAATGAGGGGCACATCTATTCTACTGTGGATGATGACCATTTTAAATCC
  ACAGATGCAGAATAACTGGATCTAACTGGGTACCTGAGATATTTTACAGCTGGACCTAGT
  TTCACAATCTGTTGTCTCCAGCTCTGCATATGTCTGGCCAGGGGGCTTCTAGGAAGTAGG
  TTTCATCTATCAAATGTCTCCTCTGACTTCCTTTTGAAACTTACTGCTCTTCTGTTTTAT
  TTTGTTTTGTTTGAAGCTCAGAGGGAGATGGGCAATTGACAGGGATGCAATCCAGGGTGG
  GATTTCTTGAGGAAGTTACAAATAAGCTTGTTACAACATCAAGATAGATGGAATTGGAAG
  GATGCTACCAGGAGAGTACTTACATAGTGCTCAGGAGTTTCTCTTCTTAAAATGTTTACT
  GCTGAAAGATGAGCAGGACCAGGGCGTTATAGGCAGAGCCCTAGCCGAGAAACCTGCTGG
  CCTCTGCCTGTTTTCATTTCCCACTTTGGTTGTGTGGCATTACTTTCAGAATTGCACTTT
  CCTGCTTGTCATGACTTTTTGACACACTTGCCATGACGTGTGTTTCTGTGAACATGAAGT
  TCTGCGGTAGTGCCTCCAGGGGCAGAGGAAAAGAAGAAGTGTTACTGCGTTTTGTACAAA
  ATAAATACAGTCATATGTTTAATAAAACAGTTCTATTGTAAAAAAAAAAAAAAAAAAAAA
  A
• Restriction Sites: Please inquire
• ACCN: NM_002946
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002946.3, NP_002937.1
• RefSeq Size: 1741 bp
• RefSeq ORF: 813 bp
• Locus ID: 6118
• Cytogenetics: 1p35.3
• Domains: tRNA_anti
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Homologous recombination, Mismatch repair, Nucleotide excision repair
• Gene Summary: 'This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]'
  Transcript Variant: This variant (1) encodes isoform 1.