WASP (WAS) (NM_000377) Human Untagged Clone

CAT#: SC321241

WAS (untagged)-Human Wiskott-Aldrich syndrome (eczema-thrombocytopenia) (WAS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WAS
• Synonyms: IMD2; SCNX; THC; THC1; WASP; WASPA
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000377.1 GCCAGAGAAGACAAGGGCAGAAAGCACCATGAGTGGGGGCCCAATGGGAGGAAGGCCCGG
  GGGCCGAGGAGCACCAGCGGTTCAGCAGAACATACCCTCCACCCTCCTCCAGGACCACGA
  GAACCAGCGACTCTTTGAGATGCTTGGACGAAAATGCTTGACGCTGGCCACTGCAGTTGT
  TCAGCTGTACCTGGCGCTGCCCCCTGGAGCTGAGCACTGGACCAAGGAGCATTGTGGGGC
  TGTGTGCTTCGTGAAGGATAACCCCCAGAAGTCCTACTTCATCCGCCTTTACGGCCTTCA
  GGCTGGTCGGCTGCTCTGGGAACAGGAGCTGTACTCACAGCTTGTCTACTCCACCCCCAC
  CCCCTTCTTCCACACCTTCGCTGGAGATGACTGCCAAGCGGGGCTGAACTTTGCAGACGA
  GGACGAGGCCCAGGCCTTCCGGGCCCTCGTGCAGGAGAAGATACAAAAAAGGAATCAGAG
  GCAAAGTGGAGACAGACGCCAGCTACCCCCACCACCAACACCAGCCAATGAAGAGAGAAG
  AGGAGGGCTCCCACCCCTGCCCCTGCATCCAGGTGGAGACCAAGGAGGCCCTCCAGTGGG
  TCCGCTCTCCCTGGGGCTGGCGACAGTGGACATCCAGAACCCTGACATCACGAGTTCACG
  ATACCGTGGGCTCCCAGCACCTGGACCTAGCCCAGCTGATAAGAAACGCTCAGGGAAGAA
  GAAGATCAGCAAAGCTGATATTGGTGCACCCAGTGGATTCAAGCATGTCAGCCACGTGGG
  GTGGGACCCCCAGAATGGATTTGACGTGAACAACCTCGACCCAGATCTGCGGAGTCTGTT
  CTCCAGGGCAGGAATCAGCGAGGCCCAGCTCACCGACGCCGAGACCTCTAAACTTATCTA
  CGACTTCATTGAGGACCAGGGTGGGCTGGAGGCTGTGCGGCAGGAGATGAGGCGCCAGGA
  GCCACTTCCGCCGCCCCCACCGCCATCTCGAGGAGGGAACCAGCTCCCCCGGCCCCCTAT
  TGTGGGGGGTAACAAGGGTCGTTCTGGTCCACTGCCCCCTGTACCTTTGGGGATTGCCCC
  ACCCCCACCAACACCCCGGGGACCCCCACCCCCAGGCCGAGGGGGCCCTCCACCACCACC
  CCCTCCAGCTACTGGACGTTCTGGACCACTGCCCCCTCCACCCCCTGGAGCTGGTGGGCC
  ACCCATGCCACCACCACCGCCACCACCGCCACCGCCGCCCAGCTCCGGGAATGGACCAGC
  CCCTCCCCCACTCCCTCCTGCTCTGGTGCCTGCCGGGGGCCTGGCCCCTGGTGGGGGTCG
  GGGAGCGCTTTTGGATCAAATCCGGCAGGGAATTCAGCTGAACAAGACCCCTGGGGCCCC
  AGAGAGCTCAGCGCTGCAGCCACCACCTCAGAGCTCAGAGGGACTGGTGGGGGCCCTGAT
  GCACGTGATGCAGAAGAGAAGCAGAGCCATCCACTCCTCCGACGAAGGGGAGGACCAGGC
  TGGCGATGAAGATGAAGATGATGAATGGGATGACTGAGTGGCTGAGTTACTTGCTGCCCT
  GTGCTCCTCCCCGCAGGACATGGCTCCCCCTCCACCTGCTCTGTGCCCACCCTCCACTCT
  CCTCTTCCAGGCCCCCAACCCCCCATTTCTTCCCCACCAACCCCTCCAATGCTGTTATCC
  CTGCCTGGTCCTCACACTCACCCAACAATCCCAAGGCCCTTTTTATACAAAAATTCTCAG
  TTCTCTTCACTCAAGGATTTTTAAAGAAAAATAAAAGAATTGTCTTTCTGTCTCTCAAAA
  AAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000377
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000377.1, NP_000368.1
• RefSeq Size: 1806 bp
• RefSeq ORF: 1509 bp
• Locus ID: 7454
• Cytogenetics: Xp11.23
• Domains: PBD, WH1, WH2
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
• Gene Summary: ' The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq, Jul 2008]'