ATP1B3 (NM_001679) Human Untagged Clone

CAT#: SC321441

ATP1B3 (untagged)-Human ATPase, Na+/K+ transporting, beta 3 polypeptide (ATP1B3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP1B3
• Synonyms: ATPB-3; CD298
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001679.2 CGAGTACTCCCCGTAACGAGGAGGTGTTCTCGGCCGTCCCACCCTTCACTGCCGTCTCCG
  GGCTGCGCCGCCGGAGCCGGGACGCGCCTCCGCAGCCCTCGCCGCCTCCATCCCCGCGGC
  CGCAGCTCCTCTCGCCGTCCGCGCGCACACCATGACGAAGAACGAGAAGAAGTCCCTCAA
  CCAGAGCCTGGCCGAGTGGAAGCTCTTCATCTACAACCCGACCACCGGAGAATTCCTGGG
  GCGCACCGCCAAGAGCTGGGGTTTGATCTTGCTCTTCTACCTAGTTTTTTATGGGTTCCT
  GGCTGCACTCTTCTCATTCACGATGTGGGTTATGCTTCAGACTCTCAACGATGAGGTTCC
  AAAATACCGTGACCAGATTCCTAGCCCAGGACTCATGGTTTTTCCAAAACCAGTGACCGC
  ATTGGAATATACATTCAGTAGGTCTGATCCAACTTCGTATGCAGGGTACATTGAAGACCT
  TAAGAAGTTTCTAAAACCATATACTTTAGAAGAACAGAAGAACCTCACAGTCTGTCCTGA
  TGGAGCACTTTTTGAACAGAAGGGTCCAGTTTATGTTGCATGTCAGTTTCCTATTTCATT
  ACTTCAAGCATGCAGTGGTATGAATGATCCTGATTTTGGCTATTCTCAAGGAAACCCTTG
  TATTCTTGTGAAAATGAACAGAATAATTGGATTAAAGCCTGAAGGAGTGCCAAGGATAGA
  TTGTGTTTCAAAGAATGAAGATATACCAAATGTAGCAGTTTATCCTCATAATGGAATGAT
  AGACTTAAAATATTTCCCATATTATGGGAAAAAACTGCATGTTGGGTATCTACAGCCATT
  GGTTGCTGTTCAGGTCAGCTTTGCTCCTAACAACACTGGGAAAGAAGTAACAGTTGAGTG
  CAAGATTGATGGATCAGCCAACCTAAAAAGTCAGGATGATCGTGACAAGTTTTTGGGACG
  AGTTATGTTCAAAATCACAGCACGTGCATAGTATGAGTAGGATATCTCCACAGAGTAAAT
  GTTGTGTTGTCTGTCTTCATTTTGTAACAGCTGGACCTTCCATTCTAGAATTATGAGACC
  ACCTTGGAGAAAGGTGTGTGGTACATGACATTGGGTTACATCATAACGTGCTTCCAGATC
  ATAGTGTTCAGTGTCCTCTGAAGTAACTGCCTGTTGCCTCTGCTGCCCTTTGAACCAGTG
  TACAGTCGCCAGATAGGGACCGGTGAACACCTGATTCCAAACATGTAGGATGGGGGTCTT
  GTCCTCTTTTTATGTGGTTTAATTGCCAAGTGTCTAAAGCTTAATATGCCGTGCTATGTA
  AATATTTTATGGATATAACAACTGTCATATTTTGATGTCAACAGAGTTTTAGGGATAAAA
  TGGTACCCGGCCAACATCAAGTGACTTTATAGCTGCAAGAAATGTGGTATGTGGAGAAGT
  TCTGTATGTGAGGAAGGAAAAAAAGAAAATAAAAGTGTGTTTGAAAAATAAAAAAAAAAA
  AAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001679
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001679.2, NP_001670.1
• RefSeq Size: 1853 bp
• RefSeq ORF: 840 bp
• Locus ID: 483
• Cytogenetics: 3q23
• Domains: Na_K-ATPase
• Protein Families: Transmembrane
• Protein Pathways: Cardiac muscle contraction
• Gene Summary: 'The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2. [provided by RefSeq, Jul 2008]'