CD247 (NM_000734) Human Untagged Clone

CAT#: SC321778

CD247 (untagged)-Human CD247 molecule (CD247), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD247
• Synonyms: CD3-ZETA; CD3H; CD3Q; CD3Z; IMD25; T3Z; TCRZ
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000734.2 CGTCCCGGCCACCGCTGCCTCAGCCTCTGCCTCCCAGCCTCTTTCTGAGGGAAAGGACAA
  GATGAAGTGGAAGGCGCTTTTCACCGCGGCCATCCTGCAGGCACAGTTGCCGATTACAGA
  GGCACAGAGCTTTGGCCTGCTGGATCCCAAACTCTGCTACCTGCTGGATGGAATCCTCTT
  CATCTATGGTGTCATTCTCACTGCCTTGTTCCTGAGAGTGAAGTTCAGCAGGAGCGCAGA
  CGCCCCCGCGTACCAGCAGGGCCAGAACCAGCTCTATAACGAGCTCAATCTAGGACGAAG
  AGAGGAGTACGATGTTTTGGACAAGAGACGTGGCCGGGACCCTGAGATGGGGGGAAAGCC
  GCAGAGAAGGAAGAACCCTCAGGAAGGCCTGTACAATGAACTGCAGAAAGATAAGATGGC
  GGAGGCCTACAGTGAGATTGGGATGAAAGGCGAGCGCCGGAGGGGCAAGGGGCACGATGG
  CCTTTACCAGGGTCTCAGTACAGCCACCAAGGACACCTACGACGCCCTTCACATGCAGGC
  CCTGCCCCCTCGCTAACAGCCAGGGGATTTCACCACTCAAAGGCCAGACCTGCAGACGCC
  CAGATTATGAGACACAGGATGAAGCATTTACAACCCGGTTCACTCTTCTCAACCACTGAA
  GTATTCCCCTTTATGTACAGGATGCTTTGGTTATATTTAGCTCCAAACCTTCACACACAG
  ACTGTTGTCCCTGCACTCTTTAAGGGAGTGTACTCCCAGGGCTTACGGCCCTGGCCTTGG
  GCCCTCTGGTTTGCCGGTGGTGCAGGTAGACCTGTCTCCTGGCGGTTCCTCGTTCTCCCT
  GGGAGGCGGGCGCACTGCCTCTCACAGCTGAGTTGTTGAGTCTGTTTTGTAAAGTCCCCA
  GAGAAAGCGCAGATGCTAGCACATGCCCTAATGTCTGTATCACTCTGTGTCTGAGTGGCT
  TCACTCCTGCTGTAAATTTGGCTTCTGTTGTCACCTTCACCTCCTTTCAAGGTAACTGTA
  CTGGGCCATGTTGTGCCTCCCTGGTGAGAGGGCCGGGCAGAGGGGCAGATGGAAAGGAGC
  CTAGGCCAGGTGCAACCAGGGAGCTGCAGGGGCATGGGAAGGTGGGCGGGCAGGGGAGGG
  TCAGCCAGGGCCTGCGAGGGCAGCGGGAGCCTCCCTGCCTCAGGCCTCTGTGCCGCACCA
  TTGAACTGTACCATGTGCTACAGGGGCCAGAAGATGAACAGACTGACCTTGATGAGCTGT
  GCACAAAGTGGCATAAAAAACATGTGGTTACACAGTGTGAATAAAGTGCTGCGGAGCAAG
  AGGAGGCCGTTGATTCACTTCACGCTTTCAGCGAATGACAAAATCATCTTTGTGAAGGCC
  TCGCAGGAAGACCCAACACTTGGGACCTATAACTGCCCAGCGGACAGTGGCAGGACAGGA
  AAAACCCGTCAATGTACTAGGATACTGCTGCGTCATTACAGGGCACAGGCCATGGATGGA
  AAACGCTCTCTGCTCTGCTTTTTTTCTACTGTTTTAATTTATACTGGCATGCTAAAGCCT
  TCCTATTTTGCATAATAAATGCTTCAGTGAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000734
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000734.2, NP_000725.1
• RefSeq Size: 1674 bp
• RefSeq ORF: 492 bp
• Locus ID: 919
• Cytogenetics: 1q24.2
• Domains: ITAM
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate splice site in the mid-coding region, which results in a missing in-frame codon, as compared to variant 1. The encoded isoform (2) is thus missing an internal amino acid, as compared to isoform 1.