DUSP6 (NM_001946) Human Untagged Clone

CAT#: SC321781

DUSP6 (untagged)-Human dual specificity phosphatase 6 (DUSP6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DUSP6
• Synonyms: HH19; MKP3; PYST1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001946.2 GCCTCGCGCAGGGGGTGTGCGAGGCTGAGTCCAAGAGATAGCAAATCGAGTCTTAAATAA
  TCCGGGGAGAAAGACGCCCGGGTAGATTTGAGGTGCAGCCTTGGAGGGAGGGATTAGAAG
  CCGCTAGACTTTTTTTCCTCCCCTCTCAGTAGCACGGAGTCCGAATTAATTGGATTTCAT
  TCACTGGGGAGGAACAAAAACTATCTGGGCAGCTTCATTGAGAGAGATTCATTGACACTA
  AGAGCCAGCGGCTGCAGCTGGGTGCAGAGAGAACCTCCGGCTTTACTTCTGTCTCGTCTG
  CCCCAACCGCTAGCCTCGGCTTGGGTAAGGCGAGGCGGAATTAAACCCCGCTCCGAGAGC
  GGCAGCTTCGCGCGCGGTGCGCTCGGCCTATGCCTGCCCCGAGGGGCGTCTGGTAGGCAC
  CCCGCCCTCTCCCGCAGCTCGACCCCCATGATAGATACGCTCAGACCCGTGCCCTTCGCG
  TCGGAAATGGCGATCAGCAAGACGGTGGCGTGGCTCAACGAGCAGCTGGAGCTGGGCAAC
  GAGCGGCTGCTGCTGATGGACTGCCGGCCGCAGGAGCTATACGAGTCGTCGCACATCGAG
  TCGGCCATCAACGTGGCCATCCCGGGCATCATGCTGCGGCGCCTGCAGAAGGGTAACCTG
  CCGGTGCGCGCGCTCTTCACGCGCGGCGAGGACCGGGACCGCTTCACCCGGCGCTGTGGC
  ACCGACACAGTGGTGCTCTACGACGAGAGCAGCAGCGACTGGAACGAGAATACGGGCGGC
  GAGTCGGTGCTCGGGCTGCTGCTCAAGAAGCTCAAGGACGAGGGCTGCCGGGCGTTCTAC
  CTGGAAGGTGGCTTCAGTAAGTTCCAAGCCGAGTTCTCCCTGCATTGCGAGACCAATCTA
  GACGGCTCGTGTAGCAGCAGCTCGCCGCCGTTGCCAGTGCTGGGGCTCGGGGGCCTGCGG
  ATCAGCTCTGACTCTTCCTCGGACATCGAGTCTGACCTTGACCGAGACCCCAATAGTGCA
  ACAGACTCGGATGGTAGTCCGCTGTCCAACAGCCAGCCTTCCTTCCCAGTGGAGATCTTG
  CCCTTCCTCTACTTGGGCTGTGCCAAAGACTCCACCAACTTGGACGTGTTGGAGGAATTC
  GGCATCAAGTACATCTTGAACGTCACCCCCAATTTGCCGAATCTCTTTGAGAACGCAGGA
  GAGTTTAAATACAAGCAAATCCCCATCTCGGATCACTGGAGCCAAAACCTGTCCCAGTTT
  TTCCCTGAGGCCATTTCTTTCATAGATGAAGCCCGGGGCAAGAACTGTGGTGTCTTGGTA
  CATTGCTTGGCTGGCATTAGCCGCTCAGTCACTGTGACTGTGGCTTACCTTATGCAGAAG
  CTCAATCTGTCGATGAACGATGCCTATGACATTGTCAAAATGAAAAAATCCAACATATCC
  CCTAACTTCAACTTCATGGGTCAGCTGCTGGACTTCGAGAGGACGCTGGGACTCAGCAGC
  CCATGTGACAACAGGGTTCCAGCACAGCAGCTGTATTTTACCACCCCTTCCAACCAGAAT
  GTATACCAGGTGGACTCTCTGCAATCTACGTGAAAGACCCCACACCCCTCCTTGCTGGAA
  TGTGTCTGGCCCTTCAGCAGTTTCTCTTGGCAGCATCAGCTGGGCTGCTTTCTTTGTGTG
  TGGCCCCAGGTGTCAAAATGACACCAGCTGTCTGTACTAGACAAGGTTACCAAGTGCGGA
  ATTGGTTAATACTAACAGAGAGATTTGCTCCATTCTCTTTGGAATAACAGGACATGCTGT
  ATAGATACAGGCAGTAGGTTTGCTCTGTACCCATGTGTACAGCCTACCCATGCAGGGACT
  GGGATTCGAGGACTTCCAGGCGCATAGGGTAGAACCAAATGATAGGGTAGGAGCATGTGT
  TCTTTAGGGCCTTGTAAGGCTGTTTCCTTTTGCATCTGGAACTGACTATATAATTGTCTT
  CAATGAAGACTAATTCAATTTTGCATATAGAGGAGCCAAAGAGAGATTTCAGCTCTGTAT
  TTGTGGTATCAGTTTGGAAAAAAAAATCTGATACTCCATTTGATTATTGTAAATATTTGA
  TCTTGAATCACTTGACAGTGTTTGTTTGAATTGTGTTTGTTTTTTCCTTTGATGGGCTTA
  AAAGAAATTATCCAAAGGGAGAAAGAGCAGTATGCCACTTCTTAAAACAGAACAAAACAA
  AAAAAGAAAATTGTGCTCTTTTCTAATCCAAAGGGTATATTTGCAGCATGCTTGACTTTA
  CCAATTCTGATGACATCTTTACGGACACTATTATCACTAAGACCTTGTTATGGCGAAGTC
  TTTAGTCTTTTTCATGTATTTTCCTCATGATTTTTTCTCTTTATGTAGTTTGACTATGCC
  TTACCTTTGTAAATATTTTTGCTTGTGTTGTCGCAAAAGGGATAATCTGGGAAAGACACC
  AAATCATGGGCTCACTTTAAAAAAAGAAAGAATAAAAAAACCTTCAGCTGTGCTAAACAG
  TATATTACCTCTGTATAAAATTCTTCAGGGAGTGTCACCTCAAATGCAATACTTTGGGTT
  GGTTTCTTTCCTTTAAAAAAATTTGTATAAAACTGGAAGTGTGTGTGTGTGAGCATGGGT
  ACCCATTTGATAAGAGAAATGCATTTGATTGTGAAGAAGGGAGAGTTAAATTCTCCATTA
  TGTTCGTGGTGTAAAGTTTAGAGCTGGAATTTATTATAAGAATGTAAAACCTTAAATTAT
  TAATAAATAACTATTTTGGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001946
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001946.2, NP_001937.2
• RefSeq Size: 2842 bp
• RefSeq ORF: 1146 bp
• Locus ID: 1848
• Cytogenetics: 12q21.33
• Domains: DSPc, RHOD
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).