BID (NM_001196) Human Untagged Clone

CAT#: SC322103

BID (untagged)-Human BH3 interacting domain death agonist (BID), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BID
• Synonyms: FP497
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322103 AGAAAAAACCCGCGGGCCTGGCCGGGTGCAGGCCACCCTTGCCGGCGGATAGGAATCCCC
  GCCCACACCGTGGTCTTTCCAGCACCGCAGACACCTGCCGGCTCCTCCCGAGCGGAGCTC
  AGGGCTGACAAAGCGCGGTCAGAGCGGCCGCTTACTGGGGCTCGCCCGCTCCTTAGAGCA
  CTGGCAATGATGTGCGGATCCTCGCTGCTGCTGCTGGGAAACTGTTGAGTGGCTGAATGA
  CCCCAGGGGACCCTGGGAGAGCTCTGAAGCCCTCAGCCACCAAGTGGCTGGGCTGGCAAG
  GGTTCATTCATTCATTCAACAAATACGAATGTGCAGCGGTGCTGGGGTCATGATGGCTCG
  GTGGGCAGCGAGGGGCCGGGCCGGCTGGAGGAGCACAGTGCGGATTCTGTCGCCACTGGG
  ACACTGTGAACCAGGAGTGAGTCGGAGCTGCCGCGCTGCCCAGGCCATGGACTGTGAGGT
  CAACAACGGTTCCAGCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCT
  CCAAAGCTGTTCTGACAACAGCTTCCGCAGAGAGCTGGACGCACTGGGCCACGAGCTGCC
  AGTGCTGGCTCCCCAGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAG
  CCACTCCCGCTTGGGAAGAATAGAGGCAGATTCTGAAAGTCAAGAAGACATCATCCGGAA
  TATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCCGGGCCT
  GGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAG
  GGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAGGCCTACCCTAGAGACATGGAGAAGGA
  GAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCAAACGCCGTC
  CTTGCTCCGTGATGTCTTTCACACAACAGTGAACTTTATTAACCAGAACCTACGCACCTA
  CGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGTTCCAGAAGTGTGACTGG
  CTAAAGCTCGATGTGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCAT
  GTCAACAGCGTTCCTAGAGAAGACAGGCTGGAAGATAGCTGTGACTTCTATTTTAAAGAC
  AATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTC
  CATTTACACGTATTTGAATGGCCTTCATATCATCCACACATGAATCTGCACATCTGTAAA
  TCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTTAAAAATTAAATTGGAAA
  GCAGGTTTCAAGGAAGTAGAAACAAAATACAATTTTTTTGGTAAAAAAAAATTACTGTTT
  ATTAAAGTACAACCATAGAGGATGGTCTTACAGCAGGCAGTATCCTGTTTGAGGAAAGCA
  AGAATCAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTAT
  CTATCTATCTTAATACTAAGGAACCAACAATCTTCCTGTTTAAAAAACCACATGGCACAG
  AGATTCTGAACTAAAGTGCTGCACTCAAATGATGGGAAGTCCGGCCCCAGTACACAGGGG
  CTTGACTTTTTCAACTTCGTTTCCTTTGTTGGAGTCAAAAAGAACCACTTGTGGTTCTAA
  AAGGTGTGAAGGTGATTTAAGGGCCCAGGTCAGCCACTGTTTGTTTACAAAATCAGGTAA
  CTAACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGCC
  ACGGGTGCCAGAAGCTACTGCGATGCCCCGGGAGTTAGCCCCCTGGTAATAGCTGTAAAC
  TTCCAATTTCTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGCATCTGGAGAGAAC
  AGGTTTCTAAGCATAAAAGATGAAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGG
  TTCCCTCTCTTAGGGACAGTCAAAACCAAGTCACTTAGGTAGTACCAAAATAAATAAGGA
  AAAGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTGTTCCAGTGGTAAGCTATGTCC
  CAGGAATCAGTTTAAAAGCACGACAGTGGATGCTGGGTCCATATCACACACATTGCTGTG
  AACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATATGAGTAAGGGCAC
  TGACGGACTCATGATTTCTTCTTACCAGATGCTTTCCTGTTCTTTGAGTTTAAAATCATC
  AGAAAGGAAAAACAAACTCTATATTGTTCAGCATGCAATACATACCACGCTAGGGCTGGC
  TCAATTGAAAGTGGGCAAAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGG
  AGGCCACTGTTTGGAAATAAATCTTCCTAACACTACAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001196
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001196.2, NP_001187.1
• RefSeq Size: 2214 bp
• RefSeq ORF: 588 bp
• Locus ID: 637
• Cytogenetics: 22q11.21
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Pathways in cancer, Viral myocarditis
• Gene Summary: 'This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (2) has an alternate 5' exon and uses a downstream in-frame start codon, as compared to variant 1. The encoded isoform 2 thus has a shorter N-terminus, as compared to isoform 1.