CRY2 (NM_001127457) Human Untagged Clone

CAT#: SC323124

CRY2 (untagged)-Human cryptochrome 2 (photolyase-like) (CRY2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CRY2
• Synonyms: HCRY2; PHLL2
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001127457, the custom clone sequence may differ by one or more nucleotides
  ATGCCAGCTCCACCCGGGCGGACCCACACATGGTTCCTACTTCAGTCTCTGGAAGATTTG
  GACACAAGTTTAAGGAAACTGAACTCCCGCCTGTTTGTAGTCCGGGGACAGCCAGCCGAC
  GTGTTCCCAAGGCTGTTCAAGGAATGGGGAGTGACCCGCTTGACCTTTGAATATGACTCT
  GAACCCTTTGGGAAAGAACGGGATGCAGCCATCATGAAGATGGCCAAGGAGGCTGGTGTG
  GAAGTAGTGACGGAGAATTCTCATACCCTCTATGACCTGGACAGGATCATTGAGCTGAAT
  GGGCAGAAGCCACCCCTTACATACAAGCGCTTTCAGGCCATCATCAGCCGCATGGAGCTG
  CCCAAGAAGCCAGTGGGCTTGGTGACCAGCCAGCAGATGGAGAGCTGCAGGGCCGAGATC
  CAGGAGAACCACGACGAGACCTACGGCGTGCCCTCCCTGGAGGAGCTGGGGTTCCCCACT
  GAAGGACTTGGTCCAGCTGTCTGGCAGGGAGGAGAGACAGAAGCTCTGGCCCGCCTGGAT
  AAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGACCCCGAATGAACGCCAAC
  TCCCTCCTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGTTGTCTCTCCTGC
  CGCCTCTTCTACTACCGCCTGTGGGACCTGTATAAAAAGGTGAAGCGGAACAGCACACCT
  CCCCTCTCCCTATTTGGGCAACTCCTATGGCGAGAGTTCTTCTACACGGCAGCTACCAAC
  AACCCCAGGTTTGACCGCATGGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGC
  AATCCTGAGGCCCTGGCCAAGTGGGCTGAGGGCAAGACAGGCTTCCCTTGGATTGATGCC
  ATCATGACCCAACTGAGGCAGGAGGGCTGGATCCACCACCTGGCCCGGCATGCCGTGGCC
  TGCTTCCTGACCCGCGGGGACCTCTGGGTCAGCTGGGAGAGCGGGGTCCGGGTATTTGAT
  GAGCTGCTCCTGGATGCAGATTTCAGCGTGAACGCAGGCAGCTGGATGTGGCTGTCCTGC
  AGTGCTTTCTTCCAGCAGTTCTTCCACTGCTACTGCCCTGTGGGCTTTGGCCGTCGCACG
  GACCCCAGTGGGGACTACATCAGGCGATACCTGCCCAAATTGAAAGCGTTCCCCTCTCGA
  TACATCTATGAGCCCTGGAATGCCCCAGAGTCAATTCAGAAGGCAGCCAAGTGCATCATT
  GGTGTGGACTACCCACGGCCCATCGTCAACCATGCCGAGACCAGCCGGCTTAACATTGAA
  CGAATGAAGCAGATTTACCAGCAGCTTTCGCGCTACCGGGGACTCTGTCTACTGGCATCT
  GTCCCTTCCTGTGTGGAAGACCTCAGTCACCCTGTGGCAGAGCCCAGCTCGAGCCAGGCT
  GGCAGCATGAGCAGTGCAGGCCCAAGACCACTACCCAGTGGCCCAGCATCCCCCAAACGC
  AAGCTGGAAGCAGCCGAGGAACCACCTGGTGAAGAACTCAGCAAACGGGCCCGGGTGGCA
  GAGTTGCCAACCCCAGAGCTGCCGAGCAAGGATGCC
  
• Restriction Sites: Please inquire
• ACCN: NM_001127457
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127457.1, NP_001120929.1
• RefSeq Size: 4082 bp
• RefSeq ORF: 1599 bp
• Locus ID: 1408
• Cytogenetics: 11p11.2
• Protein Families: Druggable Genome
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: 'This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]'
  Transcript Variant: This variant (2) uses an alternate first exon which contains the predicted translation start site; the resulting protein has a distinct N-terminus.