SHC (SHC1) (NM_001130040) Human Untagged Clone

CAT#: SC323147

SHC1 (untagged)-Human SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SHC1
• Synonyms: SHC; SHCA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001130040, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCTCCTGCCCCCCAAGCCCAAGTACAATCCACTCCGGAATGAGTCTCTGTCATCGCTGGAGGAAG
  GGGCTTCTGGGTCCACCCCCCCGGAGGAGCTGCCTTCCCCATCAGCTTCATCCCTGGGGCCCATCCTGCC
  TCCTCTGCCTGGGGACGATAGTCCCACTACCCTGTGCTCCTTCTTCCCCCGGATGAGCAACCTGAGGCTG
  GCCAACCCGGCTGGGGGGCGCCCAGGGTCTAAGGGGGAGCCAGGAAGGGCAGCTGATGATGGGGAGGGGA
  TCGTAGGGGCAGCCATGCCAGACTCAGGCCCCCTACCCCTCCTCCAGGACATGAACAAGCTGAGTGGAGG
  CGGCGGGCGCAGGACTCGGGTGGAAGGGGGCCAGCTTGGGGGCGAGGAGTGGACCCGCCACGGGAGCTTT
  GTCAATAAGCCCACGCGGGGCTGGCTGCATCCCAACGACAAAGTCATGGGACCCGGGGTTTCCTACTTGG
  TTCGGTACATGGGTTGTGTGGAGGTCCTCCAGTCAATGCGTGCCCTGGACTTCAACACCCGGACTCAGGT
  CACCAGGGAGGCCATCAGTCTGGTGTGTGAGGCTGTGCCGGGTGCTAAGGGGGCGACAAGGAGGAGAAAG
  CCCTGTAGCCGCCCGCTCAGCTCTATCCTGGGGAGGAGTAACCTGAAATTTGCTGGAATGCCAATCACTC
  TCACCGTCTCCACCAGCAGCCTCAACCTCATGGCCGCAGACTGCAAACAGATCATCGCCAACCACCACAT
  GCAATCTATCTCATTTGCATCCGGCGGGGATCCGGACACAGCCGAGTATGTCGCCTATGTTGCCAAAGAC
  CCTGTGAATCAGAGAGCCTGCCACATTCTGGAGTGTCCCGAAGGGCTTGCCCAGGATGTCATCAGCACCA
  TTGGCCAGGCCTTCGAGTTGCGCTTCAAACAATACCTCAGGAACCCACCCAAACTGGTCACCCCTCATGA
  CAGGATGGCTGGCTTTGATGGCTCAGCATGGGATGAGGAGGAGGAAGAGCCACCTGACCATCAGTACTAT
  AATGACTTCCCGGGGAAGGAACCCCCCTTGGGGGGGGTGGTAGACATGAGGCTTCGGGAAGGAGCCGCTC
  CAGGGGCTGCTCGACCCACTGCACCCAATGCCCAGACCCCCAGCCACTTGGGAGCTACATTGCCTGTAGG
  ACAGCCTGTTGGGGGAGATCCAGAAGTCCGCAAACAGATGCCACCTCCACCACCCTGTCCAGCAGGCAGA
  GAGCTTTTTGATGATCCCTCCTATGTCAACGTCCAGAACCTAGACAAGGCCCGGCAAGCAGTGGGTGGTG
  CTGGGCCCCCCAATCCTGCTATCAATGGCAGTGCACCCCGGGACCTGTTTGACATGAAGCCCTTCGAAGA
  TGCTCTTCGCGTGCCTCCACCTCCCCAGTCGGTGTCCATGGCTGAGCAGCTCCGAGGGGAGCCCTGGTTC
  CATGGGAAGCTGAGCCGGCGGGAGGCTGAGGCACTGCTGCAGCTCAATGGGGACTTCCTGGTACGGGAGA
  GCACGACCACACCTGGCCAGTATGTGCTCACTGGCTTGCAGAGTGGGCAGCCTAAGCATTTGCTACTGGT
  GGACCCTGAGGGTGTGGTTCGGACTAAGGATCACCGCTTTGAAAGTGTCAGTCACCTTATCAGCTACCAC
  ATGGACAATCACTTGCCCATCATCTCTGCGGGCAGCGAACTGTGTCTACAGCAACCTGTGGAGCGGAAAC
  TGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001130040
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130040.1, NP_001123512.1
• RefSeq Size: 3496 bp
• RefSeq ORF: 1755 bp
• Locus ID: 6464
• Cytogenetics: 1q21.3
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Chemokine signaling pathway, Chronic myeloid leukemia, Dilated cardiomyopathy, ErbB signaling pathway, Focal adhesion, Glioma, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis
• Gene Summary: 'This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (3) encodes the longest isoform (3).