MARK3 (NM_001128920) Human Untagged Clone

CAT#: SC323178

MARK3 (untagged)-Human MAP/microtubule affinity-regulating kinase 3 (MARK3), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARK3
• Synonyms: CTAK1; KP78; Par-1a; PAR1A; VIPB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323178 sequence for NM_001128920 edited (data generated by NextGen Sequencing)
  
  
  ATGTCCACTAGGACCCCATTGCCAACGGTGAATGAACGAGACACTGAAAACCACACGTCACATGGAGATG
  GGCGTCAAGAAGTTACCTCTCGTACCAGCCGCTCAGGAGCTCGGTGTAGAAACTCTATAGCCTCCTGTGC
  AGATGAACAACCTCACATCGGAAACTACAGACTGTTGAAAACAATCGGCAAGGGGAATTTTGCAAAAGTA
  AAATTGGCAAGACATATCCTTACAGGCAGAGAGGTTGCAATAAAAATAATTGACAAAACTCAGTTGAATC
  CAACAAGTCTACAAAAGCTCTTCAGAGAAGTAAGAATAATGAAGATTTTAAATCATCCCAATATAGTGAA
  GTTATTCGAAGTCATTGAAACTGAAAAAACACTCTACCTAATCATGGAATATGCAAGTGGAGGTGAAGTA
  TTTGACTATTTGGTTGCACATGGCAGGATGAAGGAAAAAGAAGCAAGATCTAAATTTAGACAGATTGTGT
  CTGCAGTTCAATACTGCCATCAGAAACGGATCGTACATCGAGACCTCAAGGCTGAAAATCTATTGTTAGA
  TGCCGATATGAACATTAAAATAGCAGATTTCGGTTTTAGCAATGAATTTACTGTTGGCGGTAAACTCGAC
  ACGTTTTGTGGCAGTCCTCCATACGCAGCACCTGAGCTCTTCCAGGGCAAGAAATATGACGGGCCAGAAG
  TGGATGTGTAGAGTCTGGGGGTCATTTTATACACACTAGTCAGTGGCTCACTTCCCTTTGATGGGCAAAA
  CCTAAAGGAACTGAGAGAGAGAGTATTAAGAGGGAAATACAGAATTCCCTTCTACATGTCTACAGACTGT
  GAAAACCTTCTCAAACGTTTCCTGGTGCTAAATCCAATTAAACGCGGCACTCTAGAGCAAATCATGAAGG
  ACAGGTGGATCAATGCAGGGCATGAAGAAGATGAACTCAAACCATTTGTTGAACCAGAGCTAGACATCTC
  AGACCAAAAAAGAATAGATATTATGGTGGGAATGGGATATTCACAAGAAGAAATTCAAGAATCTCTTAGT
  AAGATGAAATACGATGAAATCACAGCTACATATTTGTTATTGGGGAGAAAATCTTCAGAGGTTAGGCCGA
  GCAGTGATCTCAACAACAGTACTGGCCAGTCTCCTCACCACAAAGTGCAGAGAAGTGTTTCTTCAAGCCA
  AAAGCAAAGACGCTACAGTGACCATGCTGGACCAGCTATTCCTTCTGTTGTGGCGTATCCGAAAAGGAGT
  CAGACCAGCACTGCAGATAGTGACCTCAAAGAAGATGGAATTTCCTCCCGGAAATCAAGTGGCAGTGCTG
  TTGGAGGAAAGGGAATTGCTCCAGCCAGTCCCATGCTTGGGAATGCAAGTAATCCTAATAAGGCGGATAT
  TCCTGAACGCAAGAAAAGCTCCACTGTCCCTAGTAGTAACACAGCATCTGGTGGAATGACACGACGAAAT
  ACTTATGTTTGCAGTGAGAGAACTACAGCTGATAGACACTCAGTGATTCAGAATGGCAAAGAAAACAGCA
  CTATTCCTGATCAGAGAACTCCAGTTGCTTCAACACACAGTATCAGTAGTGCAGCCACCCCAGATCGAAT
  CCGCTTCCCAAGAGGCACTGCCAGTCGTAGCACTTTCCACGGCCAGCCCCGGGAACGGCGAACCGCAACA
  TATAATGGCCCTCCTGCCTCTCCCAGCCTGTCCCATGAAGCCACACCATTGTCCCAGACTCGAAGCCGAG
  GCTCCACTAATCTCCTTAGTAAATTAACTTCAAAACTCACAAGGAGTCGCAATGTATCTGCTGAGCAAAA
  AGATGAAAACAAAGAAGCAAAGCCTCGATCCCTACGCTTCACCTGGAGCATGAAAACCACTAGTTCAATG
  GATCCCGGGGACATGATGCGGGAAATCCGCAAAGTGTTGGACGCCAATAACTGCGACTATGAGCAGAGGG
  AGCGCTTCTTGCTCTTCTGCGTCCACGGAGATGGGCACGCGGAGAACCTCGTGCAGTGGGAAATGGAAGT
  GTGCAAGCTGCCAAGACTGTCTCTGAACGGGGTCCGGTTTAAGCGGATATCGGGGACATCCATAGCCTTC
  AAAAATATTGCTTCCAAAATTGCCAATGAGCTAAAGCTGTAA
  
  Clone variation with respect to NM_001128920.2
  710:a=>g 1765:c=>t
• Restriction Sites: Please inquire
• ACCN: NM_001128920
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001128920.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128920.1, NP_001122392.1
• RefSeq Size: 3426 bp
• RefSeq ORF: 2142 bp
• Locus ID: 4140
• Cytogenetics: 14q32.32-q32.33
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (4) lacks two alternate in-frame exons and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (d) has the same N- and C-termini but is shorter compared to isoform a.