MNK1 (MKNK1) (NM_003684) Human Untagged Clone

CAT#: SC323330

MKNK1 (untagged)-Kinase deficient mutant (K78M) of Human MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MKNK1
• Synonyms: MNK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323330 sequence for NM_003684 edited (data generated by NextGen Sequencing)
  ATGGTATCTTCTCAAAAGTTGGAAAAACCTATAGAGATGGGCAGTAGCGAACCCCTTCCC
  ATCGCAGATGGTGACAGGAGGAGGAAGAAGAAGCGGAGGGGCCGGGCCACTGACTCCTTG
  CCAGGAAAGTTTGAAGATATGTACAAGCTGACCTCTGAATTGCTTGGAGAGGGAGCCTAT
  GCCAAAGTTCAAGGTGCCGTGAGCCTACAGAATGGCAAAGAGTATGCCGTCATGATCATC
  GAGAAACAAGCAGGGCACAGTCGGAGTAGGGTGTTTCGAGAGGTGGAGACGCTGTATCAG
  TGTCAGGGAAACAAGAACATTTTGGAGCTGATTGAGTTCTTTGAAGATGACACAAGGTTT
  TACTTGGTCTTTGAGAAATTGCAAGGAGGTTCCATCTTAGCCCACATCCAGAAGCAAAAG
  CACTTCAATGAGCGAGAAGCCAGCCGAGTGGTGCGGGACGTTGCTGCTGCCCTTGACTTC
  CTGCATACCAAAGACAAAGTCTCTCTCTGTCACCTAGGCTGGAGTGCTATGGCGCCATCA
  GGGCTCACTGCAGCCCCAACCTCCCTGGGCTCCAGTGATCCTCCCACCTCAGCCTCCCAA
  GTAGCTGGGACTACAGGCATTGCTCATCGTGATCTGAAACCAGAAAATATATTGTGTGAA
  TCTCCAGAAAAGGTGTCTCCAGTGAAAATCTGTGACTTTGACTTGGGCAGTGGGATGAAA
  CTGAACAACTCCTGTACCCCCATAACCACACCAGAGCTGACCACCCCATGTGGCTCTGCA
  GAATACATGGCCCCTGAGGTAGTGGAGGTCTTCACGGACCAGGCCACATTCTACGACAAG
  CGCTGTGACCTGTGGAGCCTGGGCGTGGTCCTCTACATCATGCTGAGTGGCTACCCACCC
  TTCGTGGGTCACTGCGGGGCCGACTGTGGCTGGGACCGGGGCGAGGTCTGCAGGGTGTGC
  CAGAACAAGCTGTTTGAAAGCATCCAGGAAGGCAAGTATGAGTTTCCTGACAAGGACTGG
  GCACACATCTCCAGTGAAGCCAAAGACCTCATCTCCAAGCTCCTGGTGCGAGATGCAAAG
  CAGAGACTTAGCGCCGCCCAAGTTCTGCAGCACCCATGGGTGCAGGGGCAAGCTCCAGAA
  AAGGGACTCCCCACGCCGCAAGTCCTCCAGAGGAACAGCAGCACAATGGACCTGACGCTC
  TTCGCAGCTGAGGCCATCGCCCTTAACCGCCAGCTATCTCAGCACGAAGAGAACGAACTA
  GCAGAGGAGCCAGAGGCACTAGCTGATGGCCTCTGCTCCATGAAGCTTTCCCCTCCCTGC
  AAGTCACGCCTGGCCCGGAGACGGGCCCTGGCCCAGGCAGGCCGTGGTGAAGACAGGAGC
  CCGCCCACAGCACTCTGA
  
  Clone variation with respect to NM_003684.4
  233 a=>t;234 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003684 unedited
  CCCGCCCGTTAGAGCAAAGGGCGGTAGGCGCTGTACGGTGGGAGGCTCTATATAAGCAGAGCTCGTTTAG
  TGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGACCGCTC
  CCCGGCGGGAGCCAGCGAAGGTTTCCATGTCAGAGGCCGATGGAGAACTGAAGATTGCCACCTACGCACA
  AAGGCCATTGAGACACTTCGTGTAGCTGGAAGACACCAACTTCCTGACAGGAGCTTTATTTCATTTGGGA
  TTTCAAGTTTACAGATGGTATCTTCTCAAAAGTTGGAAAAACCTATAGAGATGGGCAGTAGCGAACCCCT
  TCCCATCGCAGATGGTGACAGGAGGAGGAAGAAGAAGCGGAGGGGCCGGGCCACTGACTCCTTGCCAGGA
  AAGTTTGAAGATATGTACAAGCTGACCTCTGAATTGCTTGGAGAGGGAGCCTATGCCAAAGTTCACGTGC
  CGTGAGCCTACAGATGCAAGAGTATGCCGTCATGATCATCGAGAACAGCAGGCACAGTCGAGTAGGTGTT
  CGAAGAGGTGAGACCGCTTGTATCATGTCAGAACAGAACATTTGAAGCTGATGAGTTCTTGAAAATGACC
  CAAGTTTACTGTCTTGGAAATTGCAAGGAGTCATCTAGCCATTCCGAACCAAAGCCATTCATGACCGAAC
  CACCAATGTGTCGGACTTCCGTCTGCCTGAACTTCTCGATCCAAGACAAGGCTCTCTGTGACCTAGCCGG
  GATGTCATGCCTAAGGCCTACTGGACCACACCCCTGTCATAGTATCCAACTCAGCCTCAGATGATCGGGA
  TCAGAATTGCCATGTTATCGAACAGATATTGTGTACTCCGAAAGGTCCCTGGAACTGCGTCACTAGCGCG
  AGTGACGAGCAATCTG
  
  Kinase domain sequence

  >SC323330 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYATGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGACCGCTCCCCGGCGGGA
  GCCAGCGAAGGTTTCCATGTCAGAGGCCGATGGAGAACTGAAGATTGCCACCTACGCACAAAGGCCATTG
  AGACACTTCGTGTAGCTGGAAGACACCAACTTCCTGACAGGAGCT
  
• Restriction Sites: Please inquire
• ACCN: NM_003684
• ORF Size: 1398 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003684.3, NP_003675.2
• RefSeq Size: 2736
• RefSeq ORF: 1398
• Locus ID: 8569
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Insulin signaling pathway, MAPK signaling pathway
• Gene Summary: This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (1) encodes the longest isoform (1). CCDS Note: The coding region has been updated to scale back the N-terminus to one that is more supported by the available transcript data and by conservation across mammals.