PHKG1 (NM_006213) Human Untagged Clone

CAT#: SC323335

PHKG1 (untagged)-Kinase deficient mutant (K49M) of Human phosphorylase kinase, gamma 1 (muscle) (PHKG1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHKG1
• Synonyms: PHKG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006213, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCCGGGACGAGGCACTGCCGGACTCTCATTCTGCACAGGACTTCTATGAGAATTATGAGCCCAAAG
  AGATCCTGGGCAGGGGCGTTAGCAGTGTGGTCAGGCGATGCATCCACAAGCCCACGAGCCAGGAGTACGC
  CGTGAAGGTCATCGACGTCACCGGTGGAGGCAGCTTCAGCCCGGAGGAGGTGCGGGAGCTGCGAGAAGCC
  ACGCTGAAGGAGGTGGACATCCTGCGCAAGGTCTCAGGGCACCCCAACATCATACAGCTGAAGGACACTT
  ATGAGACCAACACTTTCTTCTTCTTGGTGTTTGACCTGATGAAGAGAGGGGAGCTCTTTGACTACCTCAC
  TGAGAAGGTCACCTTGAGTGAGAAGGAAACCAGAAAGATCATGCGAGCTCTGCTGGAGGTGATCTGCACC
  TTGCACAAACTCAACATCGTGCACCGGGACCTGAAGCCCGAGAACATTCTCTTGGATGACAACATGAACA
  TCAAGCTCACAGACTTTGGCTTTTCCTGCCAGCTGGAGCCGGGAGAGAGGCTGCGAGAGGTCTGCGGGAC
  CCCCAGTTACCTGGCCCCTGAGATTATCGAGTGCTCCATGAATGAGGACCACCCGGGCTACGGGAAAGAG
  GTGGACATGTGGAGCACTGGCGTCATCATGTACACGCTGCTGGCCGGCTCCCCGCCCTTCTGGCACCGGA
  AGCAGATGCTGATGCTGAGGATGATCATGAGCGGCAACTACCAGTTTGGCTCGCCCGAGTGGGATGATTA
  CTCGGACACCGTGAAGGACCTGGTCTCCCGATTCCTGGTGGTGCAACCCCAGAACCGCTACACAGCGGAA
  GAGGCCTTGGCACACCCCTTCTTCCAGCAGTACTTGGTGGAGGAAGTGCGGCACTTCAGCCCCCGGGGGA
  AGTTCAAGGTGATCGCTCTGACCGTGCTGGCTTCAGTGCGGATCTACTACCAGTACCGCCGGGTGAAGCC
  TGTGACCCGGGAGATCGTCATCCGAGACCCCTATGCCCTCCGGCCTCTGCGCCGGCTCATCGACGCCTAC
  GCTTTCCGAATCTATGGCCACTGGGTGAAGAAGGGGCAGCAGCAGAACCGGGCAGCCCTTTTCGAGAACA
  CACCCAAGGCCGTGCTCCTCTCCCTGGCCGAGGAGGACTACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_006213 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCTGCTCGGGACA
  CGGCTTCAGCCCTCTGTGGTCCCCTCTCCCCGGGGGGCTTTGGGATTCTTGTCAAGCTCCTTCAAGAGCC
  TGCAAGCACTTAACCAGCCACCCAGAGTTCCCTCACTGAAGATCTGAGCATGACCCGGGACGAGGCACTG
  CCGGACTCTCATTCTGCACAGGACTTCTATGAGAATTATGAGCCCCAAAGAGATCCTGGGCAGGGGCGTT
  AGCAGTGTGGTCAGGCGATGCATCCACAAGCCCACGAGCCCAGGAGTACCCCCGTGATGGGTCATCGACG
  TCACCGGTGGGAGGCAGCTTTCAGCCCCGGAGGAAGGTGCGGGAAGCTGCGGGAAGCCAACGCTGAGGAA
  GGTGGACATCCCTGGGCAGGGCTTAAGGGAACCCCAACACTATAACGGCTGAAGGACATTTATAAGCCCA
  CAATTTCCTCTCCCTGTGTGTTGGCCCTGTGAGAGAGAGGGGAGTTCTTGGCTTCCTTAACTAGAAGGCA
  CCTGATGAGAAGGGACCCGGAGGTCTATCGAGCTGCCTGAGTGATATGGCACTTGGCCAACTCAATCTGG
  ACGACCTGAGCCGGAAATTCTGGATACACATGACTAGCTCAAACTGCTACGCACTGACCGGAGAGCTCAA
  GTCCCGAACCGGTACTGCTGAATCCAGGTCCTAATAGAACGCACGGATGATTGAACTGCTAGTAGCTTGG
  CGTCGCCTTG
  
  Kinase domain sequence

  >SC323335 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSAAGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCTGCTCGGGACACGGCT
  TCAGCCCTCTGTGGTCCCCTCTCCCCGGGGGGCTTTGGGATTCTTGTCAAGCTCCTTCAAGAGCCTGCAA
  GCACTTAACCAGCCACCCAGAGTTCCCTCACTGAAGATCTGAGCA
  
• Restriction Sites: Please inquire
• ACCN: NM_006213
• ORF Size: 2130 bp
• Insert Size: 4700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_006213.2, NP_006204.1
• RefSeq Size: 2130
• RefSeq ORF: 2130
• Locus ID: 5260
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• Gene Summary: This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1.