SRPK2 (NM_182691) Human Untagged Clone

CAT#: SC323336

SRPK2 (untagged)-Kinase deficient mutant (K121M) of Human SFRS protein kinase 2 (SRPK2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRPK2
• Synonyms: SFRSK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323336 sequence for NM_182691 edited (data generated by NextGen Sequencing)
  ATGTCAGTTAACTCTGAGAAGTCGTCCTCTTCAGAAAGGCCGGAGCCTCAACAGAAAGCT
  CCTTTAGTTCCTCCTCCTCCACCGCCACCACCACCACCACCGCCACCTTTGCCAGACCCC
  ACACCCCCGGAGCCAGAGGAGGAGATCCTGGGATCAGATGATGAGGAGCAAGAGGACCCT
  GCGGACTACTGCAAAGGTGGATATCATCCAGTGAAAATTGGAGACCTCTTCAATGGCCGG
  TATCATGTTATTAGAAAGCTTGGATGGGGGCACTTCTCTACTGTCTGGCTGTGCTGGGAT
  ATGCAGGGGAAAAGATTTGTTGCAATGATGGTTGTAAAAAGTGCCCAGCATTATACGGAG
  ACAGCCTTGGATGAAATAAAATTGCTCAAATGTGTTCGAGAAAGTGATCCCAGTGACCCA
  AACAAAGACATGGTGGTCCAGCTCATTGACGACTTCAAGATTTCAGGCATGAATGGGATA
  CATGTCTGCATGGTCTTCGAAGTACTTGGCCACCATCTCCTCAAGTGGATCATCAAATCC
  AACTATCAAGGCCTCCCAGTACGTTGTGTGAAGAGTATCATTCGACAGGTCCTTCAAGGG
  TTAGATTACTTACACAGTAAGTGCAAGATCATTCATACTGACATAAAGCCGGAAAATATC
  TTGATGTGTGTGGATGATGCATATGTGAGAAGAATGGCAGCTGAGGCCACTGAGTGGCAG
  AAAGCAGGTGCTCCTCCTCCTTCAGGGTCTGCAGTGAGTACGGCTCCACAGCAGAAACCT
  ATAGGAAAAATATCTAAAAACAAAAAGAAAAAACTGAAAAAGAAACAGAAGAGGCAGGCT
  GAGTTATTGGAGAAGCGCCTGCAGGAGATAGAAGAATTGGAGCGAGAAGCTGAAAGGAAA
  ATAATAGAAGAAAACATCACCTCAGCTGCACCTTCCAATGACCAGGATGGCGAATACTGC
  CCAGAGGTGAAACTAAAAACAACAGGATTAGAGGAGGCGGCTGAGGCAGAGACTGCAAAG
  GACAATGGTGAAGCTGAGGACCAGGAAGAGAAAGAAGATGCTGAGAAAGAAAACATTGAA
  AAAGATGAAGATGATGTAGATCAGGAACTTGCGAACGTAGACCCTACGTGGATAGAATCA
  CCTAAAACCAATGGCCATATTGAGAATGGCCCATTCTCACTGGAGCAGCAACTGGACGAT
  GAAGATGATGATGAAGAAGACTGCCCAAATCCTGAGGAATATAATCTTGATGAGCCAAAT
  GCAGAAAGTGATTACACATATAGCAGCTCCTATGAACAATTCAATGGTGAATTGCCAAAT
  GGACGACATAAAATTCCCGAGTCACAGTTCCCAGAGTTTTCCACCTCGTTGTTCTCTGGA
  TCCTTAGAACCTGTGGCCTGCGGCTCTGTGCTTTCTGAGGGATCACCACTTACTGAGCAA
  GAGGAGAGCAGTCCATCCCATGACAGAAGCAGAACGGTTTCAGCCTCCAGTACTGGGGAT
  TTGCCAAAAGCAAAAACCCGGGCAGCTGACTTGTTGGTGAATCCCCTGGATCCGCGGAAT
  GCAGATAAAATTAGAGTAAAAATTGCTGACCTGGGAAATGCTTGTTGGGTGCATAAACAC
  TTCACGGAAGACATCCAGACGCGTCAGTACCGCTCCATAGAGGTTTTAATAGGAGCGGGG
  TACAGCACCCCTGCGGACATCTGGAGCACGGCGTGTATGGCATTTGAGCTGGCAACGGGA
  GATTATTTGTTTGAACCACATTCTGGGGAAGACTATTCCAGAGACGAAGACCACATAGCC
  CACATCATAGAGCTGCTAGGCAGTATTCCAAGGCACTTTGCTCTATCTGGAAAATATTCT
  CGGGAATTCTTCAATCGCAGAGGAGAACTGCGACACATCACCAAGCTGAAGCCCTGGAGC
  CTCTTTGATGTACTTGTGGAAAAGTATGGCTGGCCCCATGAAGATGCTGCACAGTTTACA
  GATTTCCTGATCCCGATGTTAGAAATGGTTCCAGAAAAACGAGCCTCAGCTGGCGAATGC
  CTTCGGCATCCTTGGTTGAATTCTTAG
  
  Clone variation with respect to NM_182691.1
  329 a=>t;330 a=>g;1117 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_182691 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGAGCGAGTGGA
  GGCTGCAGCCCAGCTCGTCTCGGCGCCCGCGTCGCCGTCGCGAAGCCCCCCGCCCCGCTTCCGCCGCGTC
  GGAATGAGCTCCCGGAAAGTGCTGGCCATTCAGGCCCGAAAGCGGAGGCCGAAAAGAGAGAAACATCCGA
  AAAAAAATCAAGCAGAAAGATTGAGCTGCTGATGTCAGTTTAACTCTGAAAAGTCGTCCCTCTTTCAGAA
  AAGGCCGGGAGCCCTCAAACAGAAAAGCCTCCTTTAGTTTCCTCCTCTTCAACGGGCCAACAACCAACAA
  CAACCGGCAACTTTTGCCCAGAACCCCACAACCCCCCGGGACCCAAAGAAGGGAAATCCGGGGTTCGGAT
  AATAAGGACCAAAAGACCCCCGGCGGCCAATGCAAAGGGGGGAATTACTCAATTGAAAATTGGAGACTCC
  TTAATGGGCGGGAATACAGGTTAAAGAAAACTTGGATGGGGGGGACTTTTCTCCTCTGTCTGCTGTGCCT
  GGGATGCCCGGGGAAAGAATTGGTGCGCTGTAGGTGTGTAAAATGCGCACAATATATAGAGACACCCCTG
  GAGAGAATAAAATGCGCACATGTGTGTCGAAGTGATACCCGTGACCCACACAGACATGTGGTGCCACGTC
  ATGTCGACTCTAGATTCAGCACGATGGGAACTGTCTGCTCGGGCTCGAGATATGGCCACTCTCAGTGTTA
  CATCACATCAAGGCTCCGATCTGTTGAAGAGTCTCGCAGCTCTAGGTATCTACATAGTGCGAATCTCATC
  GTCACGGAATCTGAGTGGAG
  
  Kinase domain sequence

  >SC323336 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GCGCRACTCTTCATGGCCGGTATCATGTTATTAGAAGCTTGGATGGGGGCACTTCTCTACTGTCTGGCTG
  TGCTGGGATATGCAGGGGAAAAGATTTGTTGCAATGATGGTTGTAAAAAGTGCCCAGCATTATACGGAGA
  CAGCCTTGGATGAAATAAAATTGCTCAAATGTGTTCGAGAAAGTGATCCCAGTGACCCAAACAAAGACAT
  GGTGGTCCAGCTCATTGACGACTTCAAGATTTCAGGCATGAATGG
  
• Restriction Sites: Please inquire
• ACCN: NM_182691
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182691.1, NP_872633.1
• RefSeq Size: 3737 bp
• RefSeq ORF: 2067 bp
• Locus ID: 6733
• Cytogenetics: 7q22.3
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: ''
  Transcript Variant: This variant (2) has an alternate first exon compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).