SRPK2 (NM_182691) Human Untagged Clone
CAT#: SC323336
SRPK2 (untagged)-Kinase deficient mutant (K121M) of Human SFRS protein kinase 2 (SRPK2), transcript variant 2
"NM_182691" in other vectors (8)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | SRPK2 |
Synonyms | SFRSK2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC323336 sequence for NM_182691 edited (data generated by NextGen Sequencing)
ATGTCAGTTAACTCTGAGAAGTCGTCCTCTTCAGAAAGGCCGGAGCCTCAACAGAAAGCT CCTTTAGTTCCTCCTCCTCCACCGCCACCACCACCACCACCGCCACCTTTGCCAGACCCC ACACCCCCGGAGCCAGAGGAGGAGATCCTGGGATCAGATGATGAGGAGCAAGAGGACCCT GCGGACTACTGCAAAGGTGGATATCATCCAGTGAAAATTGGAGACCTCTTCAATGGCCGG TATCATGTTATTAGAAAGCTTGGATGGGGGCACTTCTCTACTGTCTGGCTGTGCTGGGAT ATGCAGGGGAAAAGATTTGTTGCAATGATGGTTGTAAAAAGTGCCCAGCATTATACGGAG ACAGCCTTGGATGAAATAAAATTGCTCAAATGTGTTCGAGAAAGTGATCCCAGTGACCCA AACAAAGACATGGTGGTCCAGCTCATTGACGACTTCAAGATTTCAGGCATGAATGGGATA CATGTCTGCATGGTCTTCGAAGTACTTGGCCACCATCTCCTCAAGTGGATCATCAAATCC AACTATCAAGGCCTCCCAGTACGTTGTGTGAAGAGTATCATTCGACAGGTCCTTCAAGGG TTAGATTACTTACACAGTAAGTGCAAGATCATTCATACTGACATAAAGCCGGAAAATATC TTGATGTGTGTGGATGATGCATATGTGAGAAGAATGGCAGCTGAGGCCACTGAGTGGCAG AAAGCAGGTGCTCCTCCTCCTTCAGGGTCTGCAGTGAGTACGGCTCCACAGCAGAAACCT ATAGGAAAAATATCTAAAAACAAAAAGAAAAAACTGAAAAAGAAACAGAAGAGGCAGGCT GAGTTATTGGAGAAGCGCCTGCAGGAGATAGAAGAATTGGAGCGAGAAGCTGAAAGGAAA ATAATAGAAGAAAACATCACCTCAGCTGCACCTTCCAATGACCAGGATGGCGAATACTGC CCAGAGGTGAAACTAAAAACAACAGGATTAGAGGAGGCGGCTGAGGCAGAGACTGCAAAG GACAATGGTGAAGCTGAGGACCAGGAAGAGAAAGAAGATGCTGAGAAAGAAAACATTGAA AAAGATGAAGATGATGTAGATCAGGAACTTGCGAACGTAGACCCTACGTGGATAGAATCA CCTAAAACCAATGGCCATATTGAGAATGGCCCATTCTCACTGGAGCAGCAACTGGACGAT GAAGATGATGATGAAGAAGACTGCCCAAATCCTGAGGAATATAATCTTGATGAGCCAAAT GCAGAAAGTGATTACACATATAGCAGCTCCTATGAACAATTCAATGGTGAATTGCCAAAT GGACGACATAAAATTCCCGAGTCACAGTTCCCAGAGTTTTCCACCTCGTTGTTCTCTGGA TCCTTAGAACCTGTGGCCTGCGGCTCTGTGCTTTCTGAGGGATCACCACTTACTGAGCAA GAGGAGAGCAGTCCATCCCATGACAGAAGCAGAACGGTTTCAGCCTCCAGTACTGGGGAT TTGCCAAAAGCAAAAACCCGGGCAGCTGACTTGTTGGTGAATCCCCTGGATCCGCGGAAT GCAGATAAAATTAGAGTAAAAATTGCTGACCTGGGAAATGCTTGTTGGGTGCATAAACAC TTCACGGAAGACATCCAGACGCGTCAGTACCGCTCCATAGAGGTTTTAATAGGAGCGGGG TACAGCACCCCTGCGGACATCTGGAGCACGGCGTGTATGGCATTTGAGCTGGCAACGGGA GATTATTTGTTTGAACCACATTCTGGGGAAGACTATTCCAGAGACGAAGACCACATAGCC CACATCATAGAGCTGCTAGGCAGTATTCCAAGGCACTTTGCTCTATCTGGAAAATATTCT CGGGAATTCTTCAATCGCAGAGGAGAACTGCGACACATCACCAAGCTGAAGCCCTGGAGC CTCTTTGATGTACTTGTGGAAAAGTATGGCTGGCCCCATGAAGATGCTGCACAGTTTACA GATTTCCTGATCCCGATGTTAGAAATGGTTCCAGAAAAACGAGCCTCAGCTGGCGAATGC CTTCGGCATCCTTGGTTGAATTCTTAG Clone variation with respect to NM_182691.1 329 a=>t;330 a=>g;1117 a=>g >OriGene 5' read for mutant NM_182691 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGAGCGAGTGGA GGCTGCAGCCCAGCTCGTCTCGGCGCCCGCGTCGCCGTCGCGAAGCCCCCCGCCCCGCTTCCGCCGCGTC GGAATGAGCTCCCGGAAAGTGCTGGCCATTCAGGCCCGAAAGCGGAGGCCGAAAAGAGAGAAACATCCGA AAAAAAATCAAGCAGAAAGATTGAGCTGCTGATGTCAGTTTAACTCTGAAAAGTCGTCCCTCTTTCAGAA AAGGCCGGGAGCCCTCAAACAGAAAAGCCTCCTTTAGTTTCCTCCTCTTCAACGGGCCAACAACCAACAA CAACCGGCAACTTTTGCCCAGAACCCCACAACCCCCCGGGACCCAAAGAAGGGAAATCCGGGGTTCGGAT AATAAGGACCAAAAGACCCCCGGCGGCCAATGCAAAGGGGGGAATTACTCAATTGAAAATTGGAGACTCC TTAATGGGCGGGAATACAGGTTAAAGAAAACTTGGATGGGGGGGACTTTTCTCCTCTGTCTGCTGTGCCT GGGATGCCCGGGGAAAGAATTGGTGCGCTGTAGGTGTGTAAAATGCGCACAATATATAGAGACACCCCTG GAGAGAATAAAATGCGCACATGTGTGTCGAAGTGATACCCGTGACCCACACAGACATGTGGTGCCACGTC ATGTCGACTCTAGATTCAGCACGATGGGAACTGTCTGCTCGGGCTCGAGATATGGCCACTCTCAGTGTTA CATCACATCAAGGCTCCGATCTGTTGAAGAGTCTCGCAGCTCTAGGTATCTACATAGTGCGAATCTCATC GTCACGGAATCTGAGTGGAG >SC323336 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GCGCRACTCTTCATGGCCGGTATCATGTTATTAGAAGCTTGGATGGGGGCACTTCTCTACTGTCTGGCTG TGCTGGGATATGCAGGGGAAAAGATTTGTTGCAATGATGGTTGTAAAAAGTGCCCAGCATTATACGGAGA CAGCCTTGGATGAAATAAAATTGCTCAAATGTGTTCGAGAAAGTGATCCCAGTGACCCAAACAAAGACAT GGTGGTCCAGCTCATTGACGACTTCAAGATTTCAGGCATGAATGG |
Restriction Sites | Please inquire |
ACCN | NM_182691 |
Insert Size | 2800 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_182691.1, NP_872633.1 |
RefSeq Size | 3737 bp |
RefSeq ORF | 2067 bp |
Locus ID | 6733 |
Cytogenetics | 7q22.3 |
Protein Families | Druggable Genome, Protein Kinase |
Gene Summary | '' Transcript Variant: This variant (2) has an alternate first exon compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b). |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC107542 | SRPK2 (untagged)-Human SRSF protein kinase 2 (SRPK2), transcript variant 2 |
USD 310.00 |
|
SC322514 | SRPK2 (untagged)-Human SRSF protein kinase 2 (SRPK2), transcript variant 2 |
USD 310.00 |
|
RC205134 | SRPK2 (Myc-DDK-tagged)-Human SRSF protein kinase 2 (SRPK2), transcript variant 2 |
USD 420.00 |
|
RG205134 | SRPK2 (GFP-tagged) - Human SRSF protein kinase 2 (SRPK2), transcript variant 2 |
USD 460.00 |
|
RC205134L1 | Lenti ORF clone of Human SRSF protein kinase 2 (SRPK2), transcript variant 2, Myc-DDK-tagged |
USD 768.00 |
|
RC205134L2 | Lenti ORF clone of Human SRSF protein kinase 2 (SRPK2), transcript variant 2, mGFP tagged |
USD 620.00 |
|
RC205134L3 | Lenti ORF clone of Human SRSF protein kinase 2 (SRPK2), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC205134L4 | Lenti ORF clone of Human SRSF protein kinase 2 (SRPK2), transcript variant 2, mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review