MST3 (STK24) (NM_003576) Human Untagged Clone

CAT#: SC323356

STK24 (untagged)-Kinase deficient mutant (K65M) of Human serine/threonine kinase 24 (STE20 homolog, yeast) (STK24), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STK24
• Synonyms: HEL-S-95; MST3; MST3B; STE20; STK3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003576, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACTCCAGAGCCCAGCTTTGGGGACTGGCCTTGAATAAAAGGAGGGCCACTCTACCTCATCCTGGAG
  GGAGCACGAACCTAAAGGCAGACCCAGAAGAGCTTTTTACAAAACTAGAGAAAATTGGGAAGGGCTCCTT
  TGGAGAGGTGTTCAAAGGCATTGACAATCGGACTCAGAAAGTGGTTGCCATAAAGATCATTGATCTGGAA
  GAAGCTGAAGATGAGATAGAGGACATTCAACAAGAAATCACAGTGCTGAGTCAGTGTGACAGTCCATATG
  TAACCAAATATTATGGATCCTATCTGAAGGATACAAAATTATGGATAATAATGGAATATCTTGGTGGAGG
  CTCCGCACTAGATCTATTAGAACCTGGCCCATTAGATGAAACCCAGATCGCTACTATATTAAGAGAAATA
  CTGAAAGGACTCGATTATCTCCATTCGGAGAAGAAAATCCACAGAGACATTAAAGCGGCCAACGTCCTGC
  TGTCTGAGCATGGCGAGGTGAAGCTGGCGGACTTTGGCGTGGCTGGCCAGCTGACAGACACCCAGATCAA
  AAGGAACACCTTCGTGGGCACCCCATTCTGGATGGCACCCGAGGTCATCAAACAGTCGGCCTATGACTCG
  AAGGCAGACATCTGGTCCCTGGGCATAACAGCTATTGAACTTGCAAGAGGGGAACCACCTCATTCCGAGC
  TGCACCCCATGAAAGTTTTATTCCTCATTCCAAAGAACAACCCACCGACGTTGGAAGGAAACTACAGTAA
  ACCCCTCAAGGAGTTTGTGGAGGCCTGTTTGAATAAGGAGCCGAGCTTTAGACCCACTGCTAAGGAGTTA
  TTGAAGCACAAGTTTATACTACGCAATGCAAAGAAAACTTCCTACTTGACCGAGCTCATCGACAGGTACA
  AGAGATGGAAGGCCGAGCAGAGCCATGACGACTCGAGCTCCGAGGATTCCGACGCGGAAACAGATGGCCA
  AGCCTCGGGGGGCAGTGATTCTGGGGACTGGATCTTCACAATCCGAGAAAAAGATCCCAAGAATCTCGAG
  AATGGAGCTCTTCAGCCATCGGACTTGGACAGAAATAAGATGAAAGACATCCCAAAGAGGCCTTTCTCTC
  AGTGTTTATCTACAATTATTTCTCCTCTGTTTGCAGAGTTGAAGGAGAAGAGCCAGGCGTGCGGAGGGAA
  CTTGGGGTCCATTGAAGAGCTGCGAGGGGCCATCTACCTAGCGGAGGAGGCGTGCCCTGGCATCTCCGAC
  ACCATGGTGGCCCAGCTCGTGCAGCGGCTCCAGAGATACTCTCTAAGTGGTGGAGGAACTTCATCCCACT
  GA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003576 unedited
  CCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGCTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCGGCCGGCCC
  CGCCGCCCGAGGGCTGCGCGCGGCCCGCGGGCCTCGCCGCCCCGCGCGGATCGTCGCGGCCCGGCCGTCC
  CGTCCCAGGAAGTGGCCGTCCTGAGCGCCATGGCTCACTCCCCGGTGCAGTCGGGCCTGCCCGGCATGCA
  GAACCTAAAAGGCAGACCCAGAAGAGCTTTTTACAAAACTAAAAAAAATTGGGAAGGGCTTCTTTTGGAG
  TTGGTGTTTCAAAGGCATTTGACAATCGGAACTCAAAAGTGTGGTTGCCATAAGGATCAATTGTTCTGGG
  AGGAAGCCTGAAAAGAAAAAAAAAGGACTTTTCAACAAAAAATTCCCAGGGGCTTGATTTCATTGGGGAA
  CATTCCCTTTGGTACCAAATTTTTAGGGTTTCCAACTAAAGGGAACCAAAATTTGGGAAAAAAAAGGAAA
  TTTTTTGGGGGGAGGGCCCCGCCCAATTTTTTTTTAAACCCGGCCCCATTTATAAAAAACCCCATTATCC
  TCTATATTTTAAAAAAAAAAATCTGGAGGGGCCGTTATTTTCCCTTTTCAGAAAAAAAAAAACCCCCAAA
  AATTTTTAAGGCCCCCAATCCTCCGTGTCGCCAAATAGGGGAGAGAAAAATAGGGGAGATTTTGTGGGTG
  GGTCGCATAAAAAAAACCCCGATAAAAAGAGAGCAACTCTTCGGGCGACACCTCTGTAGGGTACCACGAG
  TGTCTCACGATATGTGTCACGAGTAGACAAGAGAA
  
  Kinase domain sequence

  >SC323356 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CGACGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCGGCCGGCCCCGCCGC
  CCGAGGGCTGCGCGCGGCCCGCGGGCCTCGCCGCCCCGCGCGGATCGTCGCGGCCCGGCCGTCCCGTCCC
  AGGAAGTGGCCGTCCTGAGCGCCATGGCTCACTCCCCGGTGCAGT
  
• Restriction Sites: Please inquire
• ACCN: NM_003576
• ORF Size: 1296 bp
• Insert Size: 2440
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003576.1, NP_003567.1
• RefSeq Size: 1970
• RefSeq ORF: 1296
• Locus ID: 8428
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a serine/threonine protein kinase that functions upstream of mitogen-activated protein kinase (MAPK) signaling. The encoded protein is cleaved into two chains by caspases; the N-terminal fragment (MST3/N) translocates to the nucleus and promotes programmed cells death. There is a pseudogene for this gene on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
  Transcript Variant: This variant (1) encodes the longest isoform (a, also known as MST3b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.