HIPK4 (NM_144685) Human Untagged Clone

CAT#: SC323367

HIPK4 (untagged)-Kinase deficient mutant (K40M) of Human homeodomain interacting protein kinase 4 (HIPK4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIPK4
• Synonyms: FLJ32818
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323367 sequence for NM_144685 edited (data generated by NextGen Sequencing)
  ATGTCCACCATCCAGTCGGAGACTGACTGCTACGACATCATCGAGGTCTTGGGCAAGGGG
  ACCTTCGGGGAGGTAGCCAAGGGCTGGCGGCGGAGCACGGGCGAGATGGTGGCCATCATG
  ATCCTCAAGAATGACGCCTACCGCAACCGCATCATCAAAAACGAGCTGAAGCTGCTGCAC
  TGCATGCGAGGCCTAGACCCTGAAGAGGCCCACGTCATCCGCTTCCTTGAGTTCTTCCAT
  GACGCCCTCAAGTTCTACCTGGTCTTTGAGCTGCTGGAGCAAAACCTTTTCGAGTTCCAG
  AAGGAGAACAACTTCGCGCCCCTCCCCGCCCGCCACATCCGTACAGTCACCCTGCAGGTG
  CTCACAGCCCTGGCCCGGCTCAAGGAGCTGGCTATCATCCACGCTGATCTCAAGCCTGAG
  AACATCATGCTGGTGGACCAGACCCGCTGCCCCTTCAGGGTCAAGGTGATTGACTTCGGA
  TCCGCCAGCATTTTCAGCGAGGTGCGCTACGTGAAGGAGCCATACATCCAGTCGCGCTTC
  TACCGGGCCCCTGAGATCCTGCTGGGGCTGCCCTTCTGCGAGAAGGTGGACGTGTGGTCC
  CTGGGCTGCGTCATGGCTGAGCTGCACCTGGGCTGGCCTCTCTACCCCGGCAACAACGAG
  TACGACCAGGTGCGCTACATCTGCGAAACCCAGGGCCTGCCCAAGCCACACCTGTTGCAC
  GCCGCCTGCAAGGCCCACCACTTCTTCAAGCGCAACCCCCACCCTGACGCTGCCAACCCC
  TGGCAGCTCAAGTCCTCGGCTGACTACCTGGCCGAGACGAAGGTGCGCCCATTGGAGCGC
  CGCAAGTATATGCTCAAGTCGTTGGACCAGATTGAGACAGTGAATGGTGGCAGTGTGGCC
  AGTCGGCTAACCTTCCCTGACCGGGAGGCGCTGGCGGAGCACGCCGACCTCAAGAGCATG
  GTGGAGCTGATCAAGCGCATGCTGACCTGGGAGTCACACGAACGCATCAGCCCCAGTGCT
  GCCCTGCGCCACCCCTTCGTGTCCATGCAGCAGCTGCGCAGTGCCCACGAGACCACCCAC
  TACTACCAGCTCTCGCTGCGCAGCTACCGCCTCTCGCTGCAAGTGGAGGGGAAGCCCCCC
  ACGCCCGTCGTGGCCGCAGAAGATGGGACCCCCTACTACTGTCTGGCTGAGGAGAAGGAG
  GCTGCGGGTATGGGCAGTGTGGCCGGCAGCAGCCCCTTCTTCCGAGAGGAGAAGGCACCA
  GGTATGCAAAGAGCCATCGACCAGCTGGATGACCTGAGTCTGCAGGAGGCTGGGCATGGG
  CTGTGGGGTGAGACCTGCACCAATGCGGTCTCCGACATGATGGTCCCCCTCAAGGCAGCC
  ATCACTGGCCACCATGTGCCCGACTCGGGCCCTGAGCCCATCCTGGCCTTCTACAGCAGC
  CGCCTGGCAGGCCGCCACAAGGCCCGCAAGCCACCTGCGGGTTCCAAGTCCGACTCCAAC
  TTCAGCAACCTCATTCGGCTGAGCCAGGTCTCGCCTGAGGATGACAGGCCCTGCCGGGGC
  AGCAGCTGGGAGGAAGGAGAGCATCTCGGGGCCTCTGCTGAGCCACTGGCCATCCTGCAG
  CGAGATGAGGATGGGCCCAACATTGACAACATGACCATGGAAGCTGAGAGGCCAGACCCT
  GAGCTCTTCGACCCCAGCAGCTGTCCTGGAGAATGGCTGAGTGAGCCAGACTGCACCCTG
  GAGAGCGTCAGGGGCCCACGGGCTCAGGGGCTCCCACCCCGCCGCTCCCACCAGCATGGT
  CCACCCCGGGGGGCCACCAGCTTCCTCCAGCATGTCACCGGGCACCACTGA
  
  Clone variation with respect to NM_144685.3
  119 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_144685 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATACATTATACGAAG
  TTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGAGCCACTCAACAGCGCTGGAAC
  CCATTCGGTGGGGCCTGGGGCCCCTCATCCCAAGCCAGGAGGGTTTCTGGGGAGGGGTGCAGCCCCCTGG
  GCAGACTGACAGTGTGGGCCTGGGGGGTTTTGGGGGTGCCAGGGAAACAGGGCCCAACCCCCTGGGGGGG
  GTGGGTTAAGTGCGGTTTTCCTTTTCCCCACTGGGGGCCCTCCGTATTTCCCCATAGGGGGGGGGGGGGG
  GGGGGGCAGAACCGGGGGACCCAGAAAAAAGGGAAAGCCGGTGTTCGAAAAGTGAGGGGGGAATTCAAGG
  AACAGGGGGAGGAAAGACCCCCCAAGAAACAAGCAGAAAGGGCGGGGGACAT
  
  Kinase domain sequence

  >SC323367 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSTTGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATG
  GATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGAGCCACTCAACAGCGCTGGAACCCATT
  CGGTGGGGCCTGGGGCCCCTCATCCCAAGCCAGGAGGGTTTCTGG
  
• Restriction Sites: Please inquire
• ACCN: NM_144685
• ORF Size: 1851 bp
• Insert Size: 2472
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_144685.3, NP_653286.2
• RefSeq Size: 2467
• RefSeq ORF: 1851
• Locus ID: 147746
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the homeodomain interacting protein kinase (HIPK) family of proteins. While other members of this family are found throughout vertebrates, this member is present only in mammals. Compared to other members of this family, the encoded protein lacks a nuclear localization signal and a C-terminal autoinhibitory domain. The encoded protein exhibits kinase activity and may phosphorylate the tumor suppressor protein p53. [provided by RefSeq, Jul 2016]